Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Gladstone researchers find method to study hidden HIV reservoirs

15.04.2003


Scientists are now one step closer to understanding how HIV hides in cells and rears its ugly head once patients stop taking combination drug therapy, which can suppress viral loads to undetectable levels. The phenomenon reflects the existence of hidden populations of latently infected cells. As a result, patients must remain on therapy for life.



Eradication of these cells could lead to a cure for HIV infection. However, researchers have been hampered by their inability to identify them.

Now Gladstone researchers have found a way to identify and study latently infected cells in the laboratory. Their work is published in the April 15 issue of the European Molecular Biology Organization Journal.


"The latent pool is considered to be the barrier to eradication," said senior author Eric Verdin, MD, senior investigator at the Gladstone Institute of Virology and Immunology and UCSF professor of medicine. "Our work is geared toward finding a way to obliterate this latent pool, which would take us closer to actually finding a cure for AIDS."

Through genetic engineering, the researchers constructed a recombinant HIV strain carrying a green fluorescent protein. Using this marker, they identified a small fraction of infected cells in which the virus was latent. These cells represented less than one percent of the infected population and had eluded purification until this study.

"Before, the study of latent infection was restricted to the analysis of rare cells circulating in the blood of infected patients. As an experimental model to dissect the molecular basis of latency, these cells were very limiting," Verdin said. "We now have a laboratory model that we can use to delve deeply into what is going on."

During infection, the HIV genome integrates into the host cell’s DNA. Transcription of the viral genome leads to production of virus. The Gladstone researchers found that, in latently infected cells, the HIV genome is integrated into transcriptionally inactive regions of DNA called heterochromatin.

Verdin and his colleagues are now trying to identify drugs that can activate latent cells and cause them to produce virus. A preliminary screen identified a number of compounds that can reactivate latent HIV in the laboratory.

"Hopefully, we will be soon in a position to test some of these compounds in an animal model infected with a virus related to HIV. This will allow us to determine whether the "flushing" of latent pools is a viable therapeutic approach in HIV infection," Verdin said.


The other authors of the study are Albert A. Jordan, PhD, and Dwayne A. Bisgrove, PhD. Both are postdoctoral fellows at Gladstone.

The Gladstone Institute of Virology and Immunology is one of three research institutes at The J. David Gladstone Institutes, a private nonprofit biomedical research institution affiliated with UCSF. The institutes are named for a prominent real estate developer who died in 1971. His will created a testamentary trust that reflected his long-standing interest in medical education and research.

Daniel Oshiro | EurekAlert!
Further information:
http://www.ucsf.edu/

More articles from Health and Medicine:

nachricht 3D images of cancer cells in the body: Medical physicists from Halle present new method
16.05.2018 | Martin-Luther-Universität Halle-Wittenberg

nachricht Better equipped in the fight against lung cancer
16.05.2018 | Friedrich-Alexander-Universität Erlangen-Nürnberg

All articles from Health and Medicine >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: LZH showcases laser material processing of tomorrow at the LASYS 2018

At the LASYS 2018, from June 5th to 7th, the Laser Zentrum Hannover e.V. (LZH) will be showcasing processes for the laser material processing of tomorrow in hall 4 at stand 4E75. With blown bomb shells the LZH will present first results of a research project on civil security.

At this year's LASYS, the LZH will exhibit light-based processes such as cutting, welding, ablation and structuring as well as additive manufacturing for...

Im Focus: Self-illuminating pixels for a new display generation

There are videos on the internet that can make one marvel at technology. For example, a smartphone is casually bent around the arm or a thin-film display is rolled in all directions and with almost every diameter. From the user's point of view, this looks fantastic. From a professional point of view, however, the question arises: Is that already possible?

At Display Week 2018, scientists from the Fraunhofer Institute for Applied Polymer Research IAP will be demonstrating today’s technological possibilities and...

Im Focus: Explanation for puzzling quantum oscillations has been found

So-called quantum many-body scars allow quantum systems to stay out of equilibrium much longer, explaining experiment | Study published in Nature Physics

Recently, researchers from Harvard and MIT succeeded in trapping a record 53 atoms and individually controlling their quantum state, realizing what is called a...

Im Focus: Dozens of binaries from Milky Way's globular clusters could be detectable by LISA

Next-generation gravitational wave detector in space will complement LIGO on Earth

The historic first detection of gravitational waves from colliding black holes far outside our galaxy opened a new window to understanding the universe. A...

Im Focus: Entangled atoms shine in unison

A team led by Austrian experimental physicist Rainer Blatt has succeeded in characterizing the quantum entanglement of two spatially separated atoms by observing their light emission. This fundamental demonstration could lead to the development of highly sensitive optical gradiometers for the precise measurement of the gravitational field or the earth's magnetic field.

The age of quantum technology has long been heralded. Decades of research into the quantum world have led to the development of methods that make it possible...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

Save the date: Forum European Neuroscience – 07-11 July 2018 in Berlin, Germany

02.05.2018 | Event News

Invitation to the upcoming "Current Topics in Bioinformatics: Big Data in Genomics and Medicine"

13.04.2018 | Event News

Unique scope of UV LED technologies and applications presented in Berlin: ICULTA-2018

12.04.2018 | Event News

 
Latest News

Designer cells: artificial enzyme can activate a gene switch

22.05.2018 | Life Sciences

PR of MCC: Carbon removal from atmosphere unavoidable for 1.5 degree target

22.05.2018 | Earth Sciences

Achema 2018: New camera system monitors distillation and helps save energy

22.05.2018 | Trade Fair News

VideoLinks
Science & Research
Overview of more VideoLinks >>>