Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

New model for vascular and tumour research

21.12.2015

VOGIM cell culture technique allows tumour growth to be observed

Two characteristic features of malignant tumours are that they form massive blood vessels and bypass the immune system. A new cell culture technique allows the processes of tumour growth to be studied directly and in real time, without the need for complex experiments using live animals.


Tumour-induced cell death and tumour zones I and II. The tumour is shown in green, damaged neurons are shown in red and cell nuclei are shown in blue.

The researchers at Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU) who developed the technique looked specifically at brain tumours. The team led by neuroscientist PD Dr. Nicolai Savaskan developed a 3D cell culture technique called the Vascular Organotypic Glioma Impact Model (VOGIM) which allows the formation of tumour blood vessels and their interaction with immune cells to be observed over a period of several days in an organotypic context.

This new method will allow new medications and therapies to be analysed directly, and enable side effects to be identified more quickly and efficiently than when using conventional cell culture techniques. VOGIM also allows the amount of animal testing required in vascular studies to be reduced. The results of the study by researchers at the Department of Neurosurgery at Universitätsklinikum Erlangen have now been published in the journal ONCOTARGET.

'VOGIM is a complex technique that allows tumours to be studied in real time and under clinically realistic conditions – without the need for live animal testing,' says Dr. Nicolai Savaskan, who led the study. In the technique tissue samples from rodent brains are infected with tumour cells expressing fluorescent reporter genes.

The growth of tumour cells and blood vessels, cell death, and the influence of medications on these developments are observed in the experiment. 'Even though VOGIM still requires some animal testing, it is a great technique. It can be used to test the effectiveness of new medications and molecules against gliomas – tumours of the central nervous system – relatively quickly and in a way that is reproducible,' Dr. Savaskan explains.

'We can also use it to study the side effects of almost any medication.' The next goal of the Erlangen-based researchers is to test new hybrid molecules from the plant and animal kingdom in this system in order to quickly develop new treatment strategies.

Brain tumours have destructive effects on the surrounding cells and blood vessels. Accumulations of serum around the tumour are a common symptom. These cause swelling in the brain and increase pressure inside the skull to dangerous levels.

In the worst case, the swelling can cause pressure on the respiratory centre above the cervical spine, quickly leading to suffocation. The cause of this swelling in the brain are pathological changes to the tumour blood vessels and disruption of the immune response to the brain tumour.

The accumulations of serum are caused by the tumour cells which damage surrounding tissue and cells, making them more permeable. The new VOGIM technique allows the tumour blood vessels and the immune system of the brain to be observed closely and the influence of new therapeutics to be studied in this context.

Original publication: Ali Ghoochani, Eduard Yakubov, Tina Sehm, Zheng Fan, Stefan Hock, Michael Buchfelder, Ilker Y. Eyüpoglu, Nicolai E. Savaskan: A versatile ex vivo technique for assaying tumor angiogenesis and microglia in the brain. ONCOTARGET 2015.
DOI: 10.18632/oncotarget.6550
Full text: http://www.impactjournals.com/oncotarget/index.php?journal=oncotarget&page=a...

Further information:
PD Dr. Nicolai Savaskan
Phone: +49 9131 8544748
nicolai.savaskan@uk-erlangen.de

Patient information:
Phone: +49 9131 8549000
krebsinformation@uk-erlangen.de

Dr. Susanne Langer | idw - Informationsdienst Wissenschaft
Further information:
http://www.fau.de/

More articles from Health and Medicine:

nachricht Satellites, airport visibility readings shed light on troops' exposure to air pollution
09.12.2016 | Veterans Affairs Research Communications

nachricht Oxygen can wake up dormant bacteria for antibiotic attacks
08.12.2016 | Penn State

All articles from Health and Medicine >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Electron highway inside crystal

Physicists of the University of Würzburg have made an astonishing discovery in a specific type of topological insulators. The effect is due to the structure of the materials used. The researchers have now published their work in the journal Science.

Topological insulators are currently the hot topic in physics according to the newspaper Neue Zürcher Zeitung. Only a few weeks ago, their importance was...

Im Focus: Significantly more productivity in USP lasers

In recent years, lasers with ultrashort pulses (USP) down to the femtosecond range have become established on an industrial scale. They could advance some applications with the much-lauded “cold ablation” – if that meant they would then achieve more throughput. A new generation of process engineering that will address this issue in particular will be discussed at the “4th UKP Workshop – Ultrafast Laser Technology” in April 2017.

Even back in the 1990s, scientists were comparing materials processing with nanosecond, picosecond and femtosesecond pulses. The result was surprising:...

Im Focus: Shape matters when light meets atom

Mapping the interaction of a single atom with a single photon may inform design of quantum devices

Have you ever wondered how you see the world? Vision is about photons of light, which are packets of energy, interacting with the atoms or molecules in what...

Im Focus: Novel silicon etching technique crafts 3-D gradient refractive index micro-optics

A multi-institutional research collaboration has created a novel approach for fabricating three-dimensional micro-optics through the shape-defined formation of porous silicon (PSi), with broad impacts in integrated optoelectronics, imaging, and photovoltaics.

Working with colleagues at Stanford and The Dow Chemical Company, researchers at the University of Illinois at Urbana-Champaign fabricated 3-D birefringent...

Im Focus: Quantum Particles Form Droplets

In experiments with magnetic atoms conducted at extremely low temperatures, scientists have demonstrated a unique phase of matter: The atoms form a new type of quantum liquid or quantum droplet state. These so called quantum droplets may preserve their form in absence of external confinement because of quantum effects. The joint team of experimental physicists from Innsbruck and theoretical physicists from Hannover report on their findings in the journal Physical Review X.

“Our Quantum droplets are in the gas phase but they still drop like a rock,” explains experimental physicist Francesca Ferlaino when talking about the...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

ICTM Conference 2017: Production technology for turbomachine manufacturing of the future

16.11.2016 | Event News

Innovation Day Laser Technology – Laser Additive Manufacturing

01.11.2016 | Event News

#IC2S2: When Social Science meets Computer Science - GESIS will host the IC2S2 conference 2017

14.10.2016 | Event News

 
Latest News

Researchers identify potentially druggable mutant p53 proteins that promote cancer growth

09.12.2016 | Life Sciences

Scientists produce a new roadmap for guiding development & conservation in the Amazon

09.12.2016 | Ecology, The Environment and Conservation

Satellites, airport visibility readings shed light on troops' exposure to air pollution

09.12.2016 | Health and Medicine

VideoLinks
B2B-VideoLinks
More VideoLinks >>>