Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Researchers’ New Method May Sharpen Microscopic Images

22.03.2013
UT Dallas researchers are developing a new low-light imaging method that could improve a number of scientific applications, including the microscopic imaging of single molecules in cancer research.

Electrical engineering professor Dr. Raimund Ober and his team recently published their findings in the journal Nature Methods. In the journal, they describe a method which minimizes the deterioration of images that can occur with conventional imaging approaches.

“Any image you take of an object is translated by the camera into pixels with added electronic noise,” Ober said. “Any distortion of an image makes it harder to obtain accurate estimates of the quantities you’re interested in.”

This method could greatly enhance the accuracy with which quantities of interest, such as the location, size, and orientation of an object, are extracted from the acquired images.

Ober and his team tackled this problem by using the EMCCD camera (a standard low-light image detector) in a highly unconventional setting. Using this method, scientists can estimate quantities of interest from the image data with substantially higher accuracy than those made with conventional low-light imaging.

“We have figured out through rigorous theoretical developments that when you run an EMCCD camera in such a way that very few photons hit each of its pixels, the resulting image is minimally corrupted by the camera noise,” he said. “Our method is about using the EMCCD camera to its fullest potential, beyond what is commonly believed to be possible by the scientific imaging community.”

By increasing the magnification of the image to reduce the number of photons detected in each image pixel, they were able to significantly reduce the camera noise and considerably lessen the deteriorative effect of pixilation.

In fact, the team managed to attain particle localization accuracy that was twofold higher than those obtained with conventional EMCCD imaging.

Ober and his team applied UAIM (Ultrahigh Accuracy Imaging Modality) to the live-cell tracking of a standard protein marker for breast cancer. By being able to accurately follow the movement of the marker, valuable insights on the biology of breast cancer could be gained.

“The tracking of individual proteins represents an important way to study cancer and other diseases at the molecular level,” Ober said. “The applications of UAIM for diagnostics and research are promising.”

The research team included Jerry Chao and Sripad Ram, post-doctoral researchers at UT Dallas, and Dr. Sally Ward, professor of immunology at UT Southwestern Medical Center.

The work was funded by the National Institutes of Health and the Cancer Prevention Research Institute of Texas.

Media Contact: Katherine Morales, 972-883-4321, kxm109320@utdallas.edu
or the Office of Media Relations, 972-883-2155, newscenter@utdallas.edu

Katherine Morales | EurekAlert!
Further information:
http://www.utdallas.edu

More articles from Medical Engineering:

nachricht 3-D visualization of the pancreas -- new tool in diabetes research
15.03.2017 | Umea University

nachricht New PET radiotracer identifies inflammation in life-threatening atherosclerosis
02.03.2017 | Society of Nuclear Medicine

All articles from Medical Engineering >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Giant Magnetic Fields in the Universe

Astronomers from Bonn and Tautenburg in Thuringia (Germany) used the 100-m radio telescope at Effelsberg to observe several galaxy clusters. At the edges of these large accumulations of dark matter, stellar systems (galaxies), hot gas, and charged particles, they found magnetic fields that are exceptionally ordered over distances of many million light years. This makes them the most extended magnetic fields in the universe known so far.

The results will be published on March 22 in the journal „Astronomy & Astrophysics“.

Galaxy clusters are the largest gravitationally bound structures in the universe. With a typical extent of about 10 million light years, i.e. 100 times the...

Im Focus: Tracing down linear ubiquitination

Researchers at the Goethe University Frankfurt, together with partners from the University of Tübingen in Germany and Queen Mary University as well as Francis Crick Institute from London (UK) have developed a novel technology to decipher the secret ubiquitin code.

Ubiquitin is a small protein that can be linked to other cellular proteins, thereby controlling and modulating their functions. The attachment occurs in many...

Im Focus: Perovskite edges can be tuned for optoelectronic performance

Layered 2D material improves efficiency for solar cells and LEDs

In the eternal search for next generation high-efficiency solar cells and LEDs, scientists at Los Alamos National Laboratory and their partners are creating...

Im Focus: Polymer-coated silicon nanosheets as alternative to graphene: A perfect team for nanoelectronics

Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are less stable. Now researchers at the Technical University of Munich (TUM) have, for the first time ever, produced a composite material combining silicon nanosheets and a polymer that is both UV-resistant and easy to process. This brings the scientists a significant step closer to industrial applications like flexible displays and photosensors.

Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are...

Im Focus: Researchers Imitate Molecular Crowding in Cells

Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to simulate these confined natural conditions in artificial vesicles for the first time. As reported in the academic journal Small, the results are offering better insight into the development of nanoreactors and artificial organelles.

Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

International Land Use Symposium ILUS 2017: Call for Abstracts and Registration open

20.03.2017 | Event News

CONNECT 2017: International congress on connective tissue

14.03.2017 | Event News

ICTM Conference: Turbine Construction between Big Data and Additive Manufacturing

07.03.2017 | Event News

 
Latest News

Argon is not the 'dope' for metallic hydrogen

24.03.2017 | Materials Sciences

Astronomers find unexpected, dust-obscured star formation in distant galaxy

24.03.2017 | Physics and Astronomy

Gravitational wave kicks monster black hole out of galactic core

24.03.2017 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>