Electrical engineering professor Dr. Raimund Ober and his team recently published their findings in the journal Nature Methods. In the journal, they describe a method which minimizes the deterioration of images that can occur with conventional imaging approaches.
“Any image you take of an object is translated by the camera into pixels with added electronic noise,” Ober said. “Any distortion of an image makes it harder to obtain accurate estimates of the quantities you’re interested in.”
This method could greatly enhance the accuracy with which quantities of interest, such as the location, size, and orientation of an object, are extracted from the acquired images.
Ober and his team tackled this problem by using the EMCCD camera (a standard low-light image detector) in a highly unconventional setting. Using this method, scientists can estimate quantities of interest from the image data with substantially higher accuracy than those made with conventional low-light imaging.
“We have figured out through rigorous theoretical developments that when you run an EMCCD camera in such a way that very few photons hit each of its pixels, the resulting image is minimally corrupted by the camera noise,” he said. “Our method is about using the EMCCD camera to its fullest potential, beyond what is commonly believed to be possible by the scientific imaging community.”
By increasing the magnification of the image to reduce the number of photons detected in each image pixel, they were able to significantly reduce the camera noise and considerably lessen the deteriorative effect of pixilation.
In fact, the team managed to attain particle localization accuracy that was twofold higher than those obtained with conventional EMCCD imaging.
Ober and his team applied UAIM (Ultrahigh Accuracy Imaging Modality) to the live-cell tracking of a standard protein marker for breast cancer. By being able to accurately follow the movement of the marker, valuable insights on the biology of breast cancer could be gained.
“The tracking of individual proteins represents an important way to study cancer and other diseases at the molecular level,” Ober said. “The applications of UAIM for diagnostics and research are promising.”
The research team included Jerry Chao and Sripad Ram, post-doctoral researchers at UT Dallas, and Dr. Sally Ward, professor of immunology at UT Southwestern Medical Center.
The work was funded by the National Institutes of Health and the Cancer Prevention Research Institute of Texas.Media Contact: Katherine Morales, 972-883-4321, firstname.lastname@example.org
Katherine Morales | EurekAlert!
Gentle sensors for diagnosing brain disorders
29.09.2016 | King Abdullah University of Science and Technology
New imaging technique in Alzheimer’s disease - opens up possibilities for new drug development
28.09.2016 | Lund University
Ultrafast lasers have introduced new possibilities in engraving ultrafine structures, and scientists are now also investigating how to use them to etch microstructures into thin glass. There are possible applications in analytics (lab on a chip) and especially in electronics and the consumer sector, where great interest has been shown.
This new method was born of a surprising phenomenon: irradiating glass in a particular way with an ultrafast laser has the effect of making the glass up to a...
Terahertz excitation of selected crystal vibrations leads to an effective magnetic field that drives coherent spin motion
Controlling functional properties by light is one of the grand goals in modern condensed matter physics and materials science. A new study now demonstrates how...
Researchers from the Institute for Quantum Computing (IQC) at the University of Waterloo led the development of a new extensible wiring technique capable of controlling superconducting quantum bits, representing a significant step towards to the realization of a scalable quantum computer.
"The quantum socket is a wiring method that uses three-dimensional wires based on spring-loaded pins to address individual qubits," said Jeremy Béjanin, a PhD...
In a paper in Scientific Reports, a research team at Worcester Polytechnic Institute describes a novel light-activated phenomenon that could become the basis for applications as diverse as microscopic robotic grippers and more efficient solar cells.
A research team at Worcester Polytechnic Institute (WPI) has developed a revolutionary, light-activated semiconductor nanocomposite material that can be used...
By forcefully embedding two silicon atoms in a diamond matrix, Sandia researchers have demonstrated for the first time on a single chip all the components needed to create a quantum bridge to link quantum computers together.
"People have already built small quantum computers," says Sandia researcher Ryan Camacho. "Maybe the first useful one won't be a single giant quantum computer...
14.10.2016 | Event News
14.10.2016 | Event News
12.10.2016 | Event News
27.10.2016 | Materials Sciences
27.10.2016 | Physics and Astronomy
27.10.2016 | Life Sciences