This profile can give valuable information about the progress of the disease. A unique feature of this so-called Surface Plasmon Resonance (SPR) technique is that it directly tests on blood serum, without complex preprocessing. A special chip will enable many parallel tests. Scientists from the University of Twente and the Radboud University Nijmegen, both in The Netherlands, will publish about the new imaging technique in the Journal of the American Chemical Society (JACS).
The scientists have run tests on the serum of 50 RA patients as well as a control group of 29 persons. Direct testing on blood serum is unique: in other techniques fluorescent labels and preprocessing is necessary to visualize the relevant proteins. The diluted serum is led over a special gold coated microchip containing a large number of spots with a specific peptide coating. Whenever these peptides interact with autoantibodies present in the serum, this process can be monitored by Surface Plasmon Resonance Imaging (SPR). Using laser light, all gold spots are scanned: the reflection of light of the spots changes whenever there is a molecular interaction on the spot. At a certain angle of light, there is no reflection at all: this is the so-called SPR dip undergoing a shift caused by the interaction. The technique goes beyond proving that autoantibodies are present: the interaction between the protein and the antibody can be monitored real-time and without any labels.
Autoantibodies are manufactured by the immune system as a reaction on the so-called citrullinated proteins playing a role in rheumatoid arthritis. On a single chip, several types of peptides can be placed, for rapid parallel screening. The next step is to investigate in what way the patient profiles help to monitor the progress of the disease. This could lead to more personalized treatment in the future. The applications are not limited to monitoring rheuma or other autoimmune diseases: SPR imaging can be used for monitoring a wide range of biomolecular interactions.
The research was led by dr. Richard Schasfoort of the BIOS Lab-on-a-chip group, part of the MESA+ Institute for Nanotechnology of the University of Twente. He has closely cooperated with the Biomolecular Chemistry group of the Radboud University Nijmegen, of Professor Ger Pruijn. The research has been financed by the Dutch Technology Foundation (STW) within a project called ‘Proteomics on a chip for monitoring autoimmune diseases’.
Wiebe van der Veen | alfa
Gentle sensors for diagnosing brain disorders
29.09.2016 | King Abdullah University of Science and Technology
New imaging technique in Alzheimer’s disease - opens up possibilities for new drug development
28.09.2016 | Lund University
Ultrafast lasers have introduced new possibilities in engraving ultrafine structures, and scientists are now also investigating how to use them to etch microstructures into thin glass. There are possible applications in analytics (lab on a chip) and especially in electronics and the consumer sector, where great interest has been shown.
This new method was born of a surprising phenomenon: irradiating glass in a particular way with an ultrafast laser has the effect of making the glass up to a...
Terahertz excitation of selected crystal vibrations leads to an effective magnetic field that drives coherent spin motion
Controlling functional properties by light is one of the grand goals in modern condensed matter physics and materials science. A new study now demonstrates how...
Researchers from the Institute for Quantum Computing (IQC) at the University of Waterloo led the development of a new extensible wiring technique capable of controlling superconducting quantum bits, representing a significant step towards to the realization of a scalable quantum computer.
"The quantum socket is a wiring method that uses three-dimensional wires based on spring-loaded pins to address individual qubits," said Jeremy Béjanin, a PhD...
In a paper in Scientific Reports, a research team at Worcester Polytechnic Institute describes a novel light-activated phenomenon that could become the basis for applications as diverse as microscopic robotic grippers and more efficient solar cells.
A research team at Worcester Polytechnic Institute (WPI) has developed a revolutionary, light-activated semiconductor nanocomposite material that can be used...
By forcefully embedding two silicon atoms in a diamond matrix, Sandia researchers have demonstrated for the first time on a single chip all the components needed to create a quantum bridge to link quantum computers together.
"People have already built small quantum computers," says Sandia researcher Ryan Camacho. "Maybe the first useful one won't be a single giant quantum computer...
14.10.2016 | Event News
14.10.2016 | Event News
12.10.2016 | Event News
25.10.2016 | Earth Sciences
25.10.2016 | Power and Electrical Engineering
25.10.2016 | Process Engineering