A team of scientists at JILA, a joint institute of the National Institute of Standards and Technology (NIST) and the University of Colorado (CU) at Boulder, has shown that by sampling a person’s breath with laser light they can detect molecules in the breath that may be markers for diseases like asthma or cancer.
While many studies have been done to showcase the potential of optical technologies for breath analysis, the JILA approach takes an important step toward demonstrating the full power of optics for this prospective medical application. Their findings are published in the latest issue of the Optical Society of America’s open-access journal Optics Express.
The technique, called cavity-enhanced direct optical frequency comb spectroscopy, may one day allow doctors to screen people for certain diseases simply by sampling their breath. “This technique can give a broad picture of many different molecules in the breath all at once,” says Jun Ye, who led the research. He is a fellow of JILA, a fellow of NIST and a professor adjoint at CU-Boulder’s Department of Physics.
Optical frequency comb spectroscopy was developed in the 1990s by Ye’s JILA colleague John L. Hall and Theodor W. Hänsch of Germany’s Max-Planck Institute (they shared the 2005 Nobel Prize in Physics with Roy J. Glauber for their invention). In the paper, Michael Thorpe, a graduate research assistant, Ye, and their colleagues describe the novel application of this technique to breath analysis. Optical comb spectroscopy is powerful enough to sort through all the molecules in human breath, Ye says, but it is also sensitive enough to find those rarest molecules that may be markers of specific diseases.
Every time we breathe in, we inhale a complex mixture of gasses—mostly nitrogen, oxygen, carbon dioxide, and water vapor, but also traces of other gasses, such as carbon monoxide, nitrous oxide, and methane. Each time we exhale, we blow out a slightly different mixture with less oxygen, more carbon dioxide, and a rich collection of more than a thousand types of other molecules—most of which are present only in trace amounts.
Some of these tracer breath molecules are biomarkers of disease. Just as bad breath may indicate dental problems, excess methylamine can be used to detect liver and kidney disease, ammonia on the breath may be a sign of renal failure, elevated acetone levels in the breath can indicate diabetes, and nitric oxide levels can be used to diagnose asthma. When many breath molecules are detected simultaneously, highly reliable and disease-specific information can be collected. For instance, asthma can be detected much more reliably when carbonyl sulfide, carbon monoxide, and hydrogen peroxide are all detected in parallel with nitric oxide. The reported approach offers exactly this kind of potential.
In the experiments performed by Ye and his colleagues, optical frequency comb spectroscopy was used to analyze the breath of several student volunteers. They showed that they could detect trace signatures of gasses like ammonia, carbon monoxide, and methane on their breath. In one of these measurements, they detected carbon monoxide in a student smoker and found that it was five times higher when compared to a non-smoking student.
The researchers had the students breathe into an optical cavity—a space between two standing mirrors. The optical cavity was designed so that when they aimed a pulsed laser light into it, the light bounced back and forth so many times that it covered a distance of several kilometers by the time it exited the cavity. This essentially allowed the light to sample the entire volume of the cavity, striking all the molecules therein. In addition, this lengthens the light-molecule interaction time thereby increasing the sensitivity. By comparing the light coming out of the cavity to the light that went in, Ye and his colleagues could determine which frequencies of light were absorbed and by how much. This information told them which molecules were present in the breath from the start. The remarkable combination of a broad spectral coverage of the entire comb and a sharp spectral resolution of individual comb lines allows them to sensitively identify many different molecules, as they show in their paper.
While the efficacy of this technique has yet to be evaluated in clinical trials, monitoring the breath for such biomarkers is an attractive approach to medicine because breath analysis is the ultimate non-invasive and low-cost procedure. Existing approaches to breath analysis are limited, because the equipment is either not selective enough to detect a diverse set of rare biomarkers, or it is not sensitive enough to detect trace amounts of the molecules exhaled in human breath. The biggest shortcoming of existing approaches is their inability to provide rapid and reliable breath measurements for many biomarkers. The new technique addresses these problems with its capability to rapidly, simultaneously, sensitively, and accurately detect many breath biomarkers. The results can qualitatively change the field of breath analysis, realizing its real potential as a low-cost, rapid, robust, and non-invasive method for health screening.
Colleen Morrison | EurekAlert!
New technique makes brain scans better
22.06.2017 | Massachusetts Institute of Technology
New technology enables effective simultaneous testing for multiple blood-borne pathogens
13.06.2017 | Elsevier
An international team of scientists has proposed a new multi-disciplinary approach in which an array of new technologies will allow us to map biodiversity and the risks that wildlife is facing at the scale of whole landscapes. The findings are published in Nature Ecology and Evolution. This international research is led by the Kunming Institute of Zoology from China, University of East Anglia, University of Leicester and the Leibniz Institute for Zoo and Wildlife Research.
Using a combination of satellite and ground data, the team proposes that it is now possible to map biodiversity with an accuracy that has not been previously...
Heatwaves in the Arctic, longer periods of vegetation in Europe, severe floods in West Africa – starting in 2021, scientists want to explore the emissions of the greenhouse gas methane with the German-French satellite MERLIN. This is made possible by a new robust laser system of the Fraunhofer Institute for Laser Technology ILT in Aachen, which achieves unprecedented measurement accuracy.
Methane is primarily the result of the decomposition of organic matter. The gas has a 25 times greater warming potential than carbon dioxide, but is not as...
Hydrogen is regarded as the energy source of the future: It is produced with solar power and can be used to generate heat and electricity in fuel cells. Empa researchers have now succeeded in decoding the movement of hydrogen ions in crystals – a key step towards more efficient energy conversion in the hydrogen industry of tomorrow.
As charge carriers, electrons and ions play the leading role in electrochemical energy storage devices and converters such as batteries and fuel cells. Proton...
Scientists from the Excellence Cluster Universe at the Ludwig-Maximilians-Universität Munich have establised "Cosmowebportal", a unique data centre for cosmological simulations located at the Leibniz Supercomputing Centre (LRZ) of the Bavarian Academy of Sciences. The complete results of a series of large hydrodynamical cosmological simulations are available, with data volumes typically exceeding several hundred terabytes. Scientists worldwide can interactively explore these complex simulations via a web interface and directly access the results.
With current telescopes, scientists can observe our Universe’s galaxies and galaxy clusters and their distribution along an invisible cosmic web. From the...
Temperature measurements possible even on the smallest scale / Molecular ruby for use in material sciences, biology, and medicine
Chemists at Johannes Gutenberg University Mainz (JGU) in cooperation with researchers of the German Federal Institute for Materials Research and Testing (BAM)...
19.06.2017 | Event News
13.06.2017 | Event News
13.06.2017 | Event News
28.06.2017 | Power and Electrical Engineering
28.06.2017 | Life Sciences
28.06.2017 | Awards Funding