Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Researchers Identify Workings of L-Form Bacteria

14.10.2009
Researchers at the Johns Hopkins Bloomberg School of Public Health have for the first time identified the genetic mechanisms involved in the formation and survival of L-form bacteria. Their findings are described in a study published October 6 in the journal PLoS ONE.

L-form bacteria, which were first discovered in the 1930s, are morphological variants of classical bacteria that lack a cell wall. Under specialized growth conditions L-form bacteria are capable of forming a typical “fried egg” colony, which resembles a fried egg rather than the smooth appearance of a classic bacteria colony.

These bacteria are believed to form in response to cell wall stress from certain antibiotics or the body’s immune attack, and are suspected to be associated with antibiotic-resistant and persistent infections, as well as certain diseases.

“Our study provides new insight about the molecular basis of L-form bacteria, which was not previously known,” said Ying Zhang, MD, PhD, senior author of the study and professor in the Bloomberg School’s W. Harry Feinstone Department of Molecular Microbiology and Immunology. “These findings establish the framework for future research on how the identified genes and pathways interact leading to L-forms. They also have important implications for understanding the emergence of antibiotic resistance and bacterial persistence and for developing new drugs and vaccines targeting such persistent L-form bacteria for improved infection control.”

According to Zhang, L-form bacteria are difficult to study because their biology and the circumstances favoring the transition of classical bacteria into L-forms are not fully understood. In addition, specialized culture conditions are required for study. Most research on L-form bacteria was largely abandoned in the 1980s before modern molecular tools could be applied, but renewed interest in L-form bacteria has recently emerged.

For the study, Zhang and colleagues William Glover, a graduate student at the Bloomberg School, and Yanqin Yang, a senior program analyst with the Johns Hopkins School of Medicine, conducted a genome-wide gene expression analysis of L-form colonies of E. coli bacteria. They identified interesting stress genes and pathways that overlap with persisters and biofilm bacteria.

Furthermore, the authors carried out mutant screens and identified three groups of mutants with varying degrees of defect in L-form bacteria formation or survival compared to classic colonies of E. coli. Mutants that showed complete lack of L-form growth belonged to pathways related to cell envelope stress, DNA repair, iron regulation and outer membrane biogenesis. The mutants could be restored to L-form growth by their respective wild type genes, confirming their role in L-form formation or survival.

Tim Parsons | Newswise Science News
Further information:
http://www.jhsph.edu

More articles from Life Sciences:

nachricht A Map of the Cell’s Power Station
18.08.2017 | Albert-Ludwigs-Universität Freiburg im Breisgau

nachricht On the way to developing a new active ingredient against chronic infections
18.08.2017 | Deutsches Zentrum für Infektionsforschung

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Fizzy soda water could be key to clean manufacture of flat wonder material: Graphene

Whether you call it effervescent, fizzy, or sparkling, carbonated water is making a comeback as a beverage. Aside from quenching thirst, researchers at the University of Illinois at Urbana-Champaign have discovered a new use for these "bubbly" concoctions that will have major impact on the manufacturer of the world's thinnest, flattest, and one most useful materials -- graphene.

As graphene's popularity grows as an advanced "wonder" material, the speed and quality at which it can be manufactured will be paramount. With that in mind,...

Im Focus: Exotic quantum states made from light: Physicists create optical “wells” for a super-photon

Physicists at the University of Bonn have managed to create optical hollows and more complex patterns into which the light of a Bose-Einstein condensate flows. The creation of such highly low-loss structures for light is a prerequisite for complex light circuits, such as for quantum information processing for a new generation of computers. The researchers are now presenting their results in the journal Nature Photonics.

Light particles (photons) occur as tiny, indivisible portions. Many thousands of these light portions can be merged to form a single super-photon if they are...

Im Focus: Circular RNA linked to brain function

For the first time, scientists have shown that circular RNA is linked to brain function. When a RNA molecule called Cdr1as was deleted from the genome of mice, the animals had problems filtering out unnecessary information – like patients suffering from neuropsychiatric disorders.

While hundreds of circular RNAs (circRNAs) are abundant in mammalian brains, one big question has remained unanswered: What are they actually good for? In the...

Im Focus: RAVAN CubeSat measures Earth's outgoing energy

An experimental small satellite has successfully collected and delivered data on a key measurement for predicting changes in Earth's climate.

The Radiometer Assessment using Vertically Aligned Nanotubes (RAVAN) CubeSat was launched into low-Earth orbit on Nov. 11, 2016, in order to test new...

Im Focus: Scientists shine new light on the “other high temperature superconductor”

A study led by scientists of the Max Planck Institute for the Structure and Dynamics of Matter (MPSD) at the Center for Free-Electron Laser Science in Hamburg presents evidence of the coexistence of superconductivity and “charge-density-waves” in compounds of the poorly-studied family of bismuthates. This observation opens up new perspectives for a deeper understanding of the phenomenon of high-temperature superconductivity, a topic which is at the core of condensed matter research since more than 30 years. The paper by Nicoletti et al has been published in the PNAS.

Since the beginning of the 20th century, superconductivity had been observed in some metals at temperatures only a few degrees above the absolute zero (minus...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Call for Papers – ICNFT 2018, 5th International Conference on New Forming Technology

16.08.2017 | Event News

Sustainability is the business model of tomorrow

04.08.2017 | Event News

Clash of Realities 2017: Registration now open. International Conference at TH Köln

26.07.2017 | Event News

 
Latest News

A Map of the Cell’s Power Station

18.08.2017 | Life Sciences

Engineering team images tiny quasicrystals as they form

18.08.2017 | Physics and Astronomy

Researchers printed graphene-like materials with inkjet

18.08.2017 | Materials Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>