Researchers at Oxford University’s Department of Biochemistry have developed methods for making RNA duplexes and single-stranded RNAs of desired length and sequence. This exciting technology is most applicable to commercial RNA providers and companies with large in-house requirements for RNA molecules as it will greatly increase cost-effectiveness.
Small interfering ribonucleic acids (siRNAs) are powerful laboratory tools for directed post- transcriptional gene expression knockdown and inhibition of viral propagation. For siRNA to be active, it is important that the overhang in the antisense strand is complementary to the target messenger RNA. Exogenous siRNA is frequently used in RNAi studies using chemically synthesised RNA oligonucleotides to identify reagents with optimal activity.
Chemical synthesis of RNAs is relatively straightforward, but can be prohibitively expensive. Intracellular expression provides a source of continuous production of RNA in the cell, but it offers little control over the quantity of the expressed RNA and the sequence length. In vitro transcription is relatively cheap and offers a good approach to synthesis of large quantities of RNA. Unfortunately, in vitro transcription is limited by specific sequence requirements that greatly reduce the number of potential target sites for siRNA selection. Highly efficient promoters cannot be used, due to the leader sequence being transcribed and incorporated into the siRNA, leading to a further disadvantage. The inclusion of these leader sequences ultimately prevents the siRNA from efficiently functioning in RNA interference.
Jennifer Johnson | alfa
Molecular Force Sensors
20.09.2017 | Max-Planck-Institut für Biochemie
Foster tadpoles trigger parental instinct in poison frogs
20.09.2017 | Veterinärmedizinische Universität Wien
Whispering gallery mode (WGM) resonators are used to make tiny micro-lasers, sensors, switches, routers and other devices. These tiny structures rely on a...
Using ultrafast flashes of laser and x-ray radiation, scientists at the Max Planck Institute of Quantum Optics (Garching, Germany) took snapshots of the briefest electron motion inside a solid material to date. The electron motion lasted only 750 billionths of the billionth of a second before it fainted, setting a new record of human capability to capture ultrafast processes inside solids!
When x-rays shine onto solid materials or large molecules, an electron is pushed away from its original place near the nucleus of the atom, leaving a hole...
For the first time, physicists have successfully imaged spiral magnetic ordering in a multiferroic material. These materials are considered highly promising candidates for future data storage media. The researchers were able to prove their findings using unique quantum sensors that were developed at Basel University and that can analyze electromagnetic fields on the nanometer scale. The results – obtained by scientists from the University of Basel’s Department of Physics, the Swiss Nanoscience Institute, the University of Montpellier and several laboratories from University Paris-Saclay – were recently published in the journal Nature.
Multiferroics are materials that simultaneously react to electric and magnetic fields. These two properties are rarely found together, and their combined...
MBM ScienceBridge GmbH successfully negotiated a license agreement between University Medical Center Göttingen (UMG) and the biotech company Tissue Systems Holding GmbH about commercial use of a multi-well tissue plate for automated and reliable tissue engineering & drug testing.
MBM ScienceBridge GmbH successfully negotiated a license agreement between University Medical Center Göttingen (UMG) and the biotech company Tissue Systems...
Pathogenic bacteria are becoming resistant to common antibiotics to an ever increasing degree. One of the most difficult germs is Pseudomonas aeruginosa, a...
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