Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Oxford Improves Production Method for Interfering RNA

29.04.2003


Researchers at Oxford University’s Department of Biochemistry have developed methods for making RNA duplexes and single-stranded RNAs of desired length and sequence. This exciting technology is most applicable to commercial RNA providers and companies with large in-house requirements for RNA molecules as it will greatly increase cost-effectiveness.



Small interfering ribonucleic acids (siRNAs) are powerful laboratory tools for directed post- transcriptional gene expression knockdown and inhibition of viral propagation. For siRNA to be active, it is important that the overhang in the antisense strand is complementary to the target messenger RNA. Exogenous siRNA is frequently used in RNAi studies using chemically synthesised RNA oligonucleotides to identify reagents with optimal activity.

Chemical synthesis of RNAs is relatively straightforward, but can be prohibitively expensive. Intracellular expression provides a source of continuous production of RNA in the cell, but it offers little control over the quantity of the expressed RNA and the sequence length. In vitro transcription is relatively cheap and offers a good approach to synthesis of large quantities of RNA. Unfortunately, in vitro transcription is limited by specific sequence requirements that greatly reduce the number of potential target sites for siRNA selection. Highly efficient promoters cannot be used, due to the leader sequence being transcribed and incorporated into the siRNA, leading to a further disadvantage. The inclusion of these leader sequences ultimately prevents the siRNA from efficiently functioning in RNA interference.


The methods of the Oxford invention rely on cleavage of primary single-stranded RNAs at a defined position in order to generate RNA strands of a required length and sequence. If it is desired to produce an RNA duplex, then two such RNA strands having complementary sequence, over at least a portion of their length, may be synthesised and then annealed to form an RNA duplex.

Isis Innovation, Oxford University’s technology transfer company, has filed a patent on this exciting technology and is actively seeking partners to commercialise it. Incorporated into the production process of commercial providers of RNA, the Oxford technology would provide huge potential benefits, greatly reducing the cost per nucleotide for RNA products.

Jennifer Johnson | alfa
Further information:
http://www.isis-innovation.com/licensing/1262.html
http://nar.oupjournals.org/cgi/content/full/31/7/e38

More articles from Life Sciences:

nachricht More genes are active in high-performance maize
19.01.2018 | Rheinische Friedrich-Wilhelms-Universität Bonn

nachricht How plants see light
19.01.2018 | Albert-Ludwigs-Universität Freiburg im Breisgau

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Artificial agent designs quantum experiments

On the way to an intelligent laboratory, physicists from Innsbruck and Vienna present an artificial agent that autonomously designs quantum experiments. In initial experiments, the system has independently (re)discovered experimental techniques that are nowadays standard in modern quantum optical laboratories. This shows how machines could play a more creative role in research in the future.

We carry smartphones in our pockets, the streets are dotted with semi-autonomous cars, but in the research laboratory experiments are still being designed by...

Im Focus: Scientists decipher key principle behind reaction of metalloenzymes

So-called pre-distorted states accelerate photochemical reactions too

What enables electrons to be transferred swiftly, for example during photosynthesis? An interdisciplinary team of researchers has worked out the details of how...

Im Focus: The first precise measurement of a single molecule's effective charge

For the first time, scientists have precisely measured the effective electrical charge of a single molecule in solution. This fundamental insight of an SNSF Professor could also pave the way for future medical diagnostics.

Electrical charge is one of the key properties that allows molecules to interact. Life itself depends on this phenomenon: many biological processes involve...

Im Focus: Paradigm shift in Paris: Encouraging an holistic view of laser machining

At the JEC World Composite Show in Paris in March 2018, the Fraunhofer Institute for Laser Technology ILT will be focusing on the latest trends and innovations in laser machining of composites. Among other things, researchers at the booth shared with the Aachen Center for Integrative Lightweight Production (AZL) will demonstrate how lasers can be used for joining, structuring, cutting and drilling composite materials.

No other industry has attracted as much public attention to composite materials as the automotive industry, which along with the aerospace industry is a driver...

Im Focus: Room-temperature multiferroic thin films and their properties

Scientists at Tokyo Institute of Technology (Tokyo Tech) and Tohoku University have developed high-quality GFO epitaxial films and systematically investigated their ferroelectric and ferromagnetic properties. They also demonstrated the room-temperature magnetocapacitance effects of these GFO thin films.

Multiferroic materials show magnetically driven ferroelectricity. They are attracting increasing attention because of their fascinating properties such as...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

10th International Symposium: “Advanced Battery Power – Kraftwerk Batterie” Münster, 10-11 April 2018

08.01.2018 | Event News

See, understand and experience the work of the future

11.12.2017 | Event News

Innovative strategies to tackle parasitic worms

08.12.2017 | Event News

 
Latest News

More genes are active in high-performance maize

19.01.2018 | Life Sciences

How plants see light

19.01.2018 | Life Sciences

Artificial agent designs quantum experiments

19.01.2018 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>