Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Protein expression gets the heart pumping

23.04.2014

Most people think the development of the heart only happens in the womb, however the days and weeks following birth are full of cellular changes that play a role in the structure and function of the heart.

Using mouse models, researchers at Baylor College of Medicine have now been able to categorize the alternative splicing (the process in which genes code proteins, determining their role) that takes place during these changes and what mechanisms they affect.

The findings, which appear in Nature Communications, also helped to identify a protein that regulates some of the alternative splicing and then goes on to change dramatically in its expression during the postnatal period.

"The cells of the heart stop dividing after birth but they have to continue growing and working together for the heart to pump the blood. So basically, we have made the connection between the process of alternative splicing and the development of this system that coordinates heart contraction and function," said Thomas Cooper, the S. Donald Greenberg professor of pathology & immunology at Baylor.

Researchers were able to separate two main cell types of the mouse heart, the cardiomyocytes and cardiac fibroblasts. Using RNA sequencing they looked at early- and late-stage development within the days following birth. RNA sequencing is a technique that reveals the messages transmitted to the cell from the genome, allowing researchers to see the mechanisms associated with gene expression. During the sequencing, Cooper and his colleagues were able to see what genes are turned on and off and which ones undergo an alternative splicing change.

By pinpointing these changes, the team of researchers identified the CELF1 protein as being responsible for regulating certain alternative splicing events, Cooper said. So by turning on and off CELF1 expression at different points in development, researchers were able to see how the protein affects development during this stage.

"We looked at hundreds of genes that undergo alternative splicing and were able to see which ones are regulated by CELF1," Cooper said. "We asked if is there anything in common among these genes and found that some were responsible for endocytosis and vesicular trafficking. So what is going on in heart development that is related to these processes associated with cell membrane dynamics?"

It turns out, Cooper said, that the cell membrane machinery that is required to coordinate contraction, the electrical activity of the heart, all develops in this postnatal period.

There are some ailments that CELF1 is associated with such as arrhythmias and some forms of muscular dystrophy and Cooper said it is possible that this protein could provide a treatment target.

"Now we know what happens during this period in terms of what genes are on and off and what alternative splicing takes place. This is new information for further studies to build on," he said. "There is still information about this developmental stage that must be looked at first."

###

This work was performed by Jimena Guidice, a postdoctoral fellow in Cooper's lab. Others who contributed to this research include Zheng Xia, Marissa A. Scavuzzo, Amanda J. Ward, Auinash Kalsotra, Wei Wang, Xander H.T. Wehrens, Wei Li, all of Baylor College of Medicine; and Eric T. Wang and Christopher B. Burge, both with Massachusetts institute of Technology. Ward and Kalsotra are currently with Isis Pharmaceuticals, Carlsbad California.

Funding for this research is from the National Institutes of Health (R01HL045565, R01AR060733, and R01AR045653), the Muscular Dystrophy Association, the Pew Charitable Trusts, the Myotonic Dystrophy Foundation, the American Heart Association, the National Institute of Neurological Disorders and Stroke, CPRIT and Foundation Leducq.

Graciela Gutierrez | Eurek Alert!

Further reports about: Heart Medicine Protein RNA activity function genes mechanisms protein pump splicing

More articles from Life Sciences:

nachricht The balancing act: An enzyme that links endocytosis to membrane recycling
07.12.2016 | National Centre for Biological Sciences

nachricht Transforming plant cells from generalists to specialists
07.12.2016 | Duke University

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Significantly more productivity in USP lasers

In recent years, lasers with ultrashort pulses (USP) down to the femtosecond range have become established on an industrial scale. They could advance some applications with the much-lauded “cold ablation” – if that meant they would then achieve more throughput. A new generation of process engineering that will address this issue in particular will be discussed at the “4th UKP Workshop – Ultrafast Laser Technology” in April 2017.

Even back in the 1990s, scientists were comparing materials processing with nanosecond, picosecond and femtosesecond pulses. The result was surprising:...

Im Focus: Shape matters when light meets atom

Mapping the interaction of a single atom with a single photon may inform design of quantum devices

Have you ever wondered how you see the world? Vision is about photons of light, which are packets of energy, interacting with the atoms or molecules in what...

Im Focus: Novel silicon etching technique crafts 3-D gradient refractive index micro-optics

A multi-institutional research collaboration has created a novel approach for fabricating three-dimensional micro-optics through the shape-defined formation of porous silicon (PSi), with broad impacts in integrated optoelectronics, imaging, and photovoltaics.

Working with colleagues at Stanford and The Dow Chemical Company, researchers at the University of Illinois at Urbana-Champaign fabricated 3-D birefringent...

Im Focus: Quantum Particles Form Droplets

In experiments with magnetic atoms conducted at extremely low temperatures, scientists have demonstrated a unique phase of matter: The atoms form a new type of quantum liquid or quantum droplet state. These so called quantum droplets may preserve their form in absence of external confinement because of quantum effects. The joint team of experimental physicists from Innsbruck and theoretical physicists from Hannover report on their findings in the journal Physical Review X.

“Our Quantum droplets are in the gas phase but they still drop like a rock,” explains experimental physicist Francesca Ferlaino when talking about the...

Im Focus: MADMAX: Max Planck Institute for Physics takes up axion research

The Max Planck Institute for Physics (MPP) is opening up a new research field. A workshop from November 21 - 22, 2016 will mark the start of activities for an innovative axion experiment. Axions are still only purely hypothetical particles. Their detection could solve two fundamental problems in particle physics: What dark matter consists of and why it has not yet been possible to directly observe a CP violation for the strong interaction.

The “MADMAX” project is the MPP’s commitment to axion research. Axions are so far only a theoretical prediction and are difficult to detect: on the one hand,...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

ICTM Conference 2017: Production technology for turbomachine manufacturing of the future

16.11.2016 | Event News

Innovation Day Laser Technology – Laser Additive Manufacturing

01.11.2016 | Event News

#IC2S2: When Social Science meets Computer Science - GESIS will host the IC2S2 conference 2017

14.10.2016 | Event News

 
Latest News

NTU scientists build new ultrasound device using 3-D printing technology

07.12.2016 | Health and Medicine

The balancing act: An enzyme that links endocytosis to membrane recycling

07.12.2016 | Life Sciences

How to turn white fat brown

07.12.2016 | Health and Medicine

VideoLinks
B2B-VideoLinks
More VideoLinks >>>