Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

New 3D test system for breast cancer radiosensitizing drugs

23.06.2015

Radiation therapy is an important part of breast cancer treatment. Sometimes, however, tumor cells become increasingly resistant to radiation damage. Now, scientists of Helmholtz Zentrum München have developed a 3D microtissue-based screening system which, for the first time, enables them to test which chemotherapeutic drugs can resensitize breast cancer cells to radiation.

The team of researchers led by Dr. Nataša Anastasov, head of the research group ‘Personalized Radiation Therapy’ at the Institute of Radiation Biology, created multicellular three-dimensional spheroids containing a mix of breast cancer cells and connective tissue cells.


Picture: Hanging Drop System for the production of 3D micro tissue

Source: InSphero AG (2014)

As Anastasov explained, “In conventional cell culture, cell growth is two dimensional, which does not adequately reproduce the characteristics of a tumor in the living organism.” Using the new screening system, the scientists can treat the cancer cells both with various chemical compounds and with radiation to determine the effects – and that in 3D.

In their study the researchers collaborated closely with the ‘Assay Development and Screening Platform’ at Helmholtz Zentrum München and the Munich-based Sirion Biotech GmbH and the Swiss InSphero AG.

The trick: fluorescent cells in hanging drops

“In our system, up to 96 wells containing microtissue spheroids can be simultaneously monitored by a computer,” said co-author Dr. Ines Höfig, explaining the fundamental principle. “For this purpose, the 3D-microtissue spheroids were grown in a hanging drop culture on the underside of a special perforated plate. After a few days, these were transferred onto an assay plate.”

Her colleague Vanja Radulović added: “Because the breast tumor cells and the connective tissue cells have been labeled with different fluorescent markers, they fluoresce in different colors. A software program can thus capture precisely how each of the spheroids reacts to the treatment.”

To confirm their method, the scientists tested the properties of several drugs which are already used in tumor therapy. The drug vinblastine proved to be the most effective in combination with radiation. In the future, the researchers plan to expand their system by adding other components of the tumor environment such as stromal or immune cells in order to optimally simulate the situation in the patient.

Prof. Dr. Michael Atkinson, director of the Institute of Radiation Biology, views the findings of his colleagues with optimism: “For the first time a convenient 3D test for high throughput screening is available with which new drug compounds developed to resensitize tumor cells to radiation treatment can be tested. This will speed up future initiatives for drug development and will also enable the screening of established drugs in combination with radiation.”


Further Information

Background:
The rapid evolution of resistance to both conventional and small molecule therapies is a challenging problem in oncology. One approach to overcome resistance is to use combinatorial treatments that exploit their synergies. The combination of chemotherapy and radiation treatment is a potentially effective combinatorial procedure, although the optimal mix has not been identified. A major drawback in identifying potentially radiation-sensitizing active agents is the lack of high throughput screening (HTS) vehicles. These are needed to replace conventional clonogenic survival assays of radiation treatment as these are too time consuming to operate in a first-pass screening mode. Moreover, there are growing concerns that monolayer and monotypic cellular screening assays may not effectively reproduce the response of a three-dimensional solid tumor to pharmacological compounds.

Original Publication:
Anastasov, N. et al. (2015). A 3D-microtissue-based phenotypic screening of radiation resistant tumor cells with synchronized chemotherapeutic treatment, BMC Cancer, DOI: 10.1186/s12885-015-1481-9

As German Research Center for Environmental Health, Helmholtz Zentrum München pursues the goal of developing personalized medical approaches for the prevention and therapy of major common diseases such as diabetes mellitus and lung diseases. To achieve this, it investigates the interaction of genetics, environmental factors and lifestyle. The Helmholtz Zentrum München has about 2,300 staff members and is headquartered in Neuherberg in the north of Munich. Helmholtz Zentrum München is a member of the Helmholtz Association, a community of 18 scientific-technical and medical-biological research centers with a total of about 37,000 staff members.

The research of the Institute of Radiation Biology (ISB) focuses on understanding the effects of low dose radiation exposure and on studies to increase the effectiveness and specificity of tumor radiotherapy. The research program is carried out by four interlinked research groups. The ISB is part of the Department of Radiation Sciences (DRS).

Contact for the media:
Department of Communication, Helmholtz Zentrum München – German Research Center for Environmental Health (GmbH), Ingolstädter Landstr. 1, 85764 Neuherberg - Phone: +49-(0)89-3187-2238 - Fax: +49 89-3187-3324 - Email: presse@helmholtz-muenchen.de

Scientific contact at Helmholtz Zentrum München:
Dr. Nataša Anastasov, Institute of Radiation Biology, Helmholtz Zentrum München - German Research Center for Environmental Health (GmbH), Ingolstädter Landstr. 1, 85764 Neuherberg - Phone: +49-(0)89-3187-3798 - Fax: +49-(0)89-3187-3378 - E-Mail: natasa.anastasov@helmholtz-muenchen.de

Weitere Informationen:

http://www.helmholtz-muenchen.de/en/index.html - Website Helmholtz Zentrum München
http://www.helmholtz-muenchen.de/en/isb/index.html - Website Institute of Radiation Biology
http://www.helmholtz-muenchen.de/aktuelles/pressemitteilungen/2015/index.html - Press Releases of the Helmholtz Zentrum München

Helmholtz Kommunikation | Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt

More articles from Life Sciences:

nachricht Fingerprint' technique spots frog populations at risk from pollution
27.03.2017 | Lancaster University

nachricht Parallel computation provides deeper insight into brain function
27.03.2017 | Okinawa Institute of Science and Technology (OIST) Graduate University

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Giant Magnetic Fields in the Universe

Astronomers from Bonn and Tautenburg in Thuringia (Germany) used the 100-m radio telescope at Effelsberg to observe several galaxy clusters. At the edges of these large accumulations of dark matter, stellar systems (galaxies), hot gas, and charged particles, they found magnetic fields that are exceptionally ordered over distances of many million light years. This makes them the most extended magnetic fields in the universe known so far.

The results will be published on March 22 in the journal „Astronomy & Astrophysics“.

Galaxy clusters are the largest gravitationally bound structures in the universe. With a typical extent of about 10 million light years, i.e. 100 times the...

Im Focus: Tracing down linear ubiquitination

Researchers at the Goethe University Frankfurt, together with partners from the University of Tübingen in Germany and Queen Mary University as well as Francis Crick Institute from London (UK) have developed a novel technology to decipher the secret ubiquitin code.

Ubiquitin is a small protein that can be linked to other cellular proteins, thereby controlling and modulating their functions. The attachment occurs in many...

Im Focus: Perovskite edges can be tuned for optoelectronic performance

Layered 2D material improves efficiency for solar cells and LEDs

In the eternal search for next generation high-efficiency solar cells and LEDs, scientists at Los Alamos National Laboratory and their partners are creating...

Im Focus: Polymer-coated silicon nanosheets as alternative to graphene: A perfect team for nanoelectronics

Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are less stable. Now researchers at the Technical University of Munich (TUM) have, for the first time ever, produced a composite material combining silicon nanosheets and a polymer that is both UV-resistant and easy to process. This brings the scientists a significant step closer to industrial applications like flexible displays and photosensors.

Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are...

Im Focus: Researchers Imitate Molecular Crowding in Cells

Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to simulate these confined natural conditions in artificial vesicles for the first time. As reported in the academic journal Small, the results are offering better insight into the development of nanoreactors and artificial organelles.

Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

International Land Use Symposium ILUS 2017: Call for Abstracts and Registration open

20.03.2017 | Event News

CONNECT 2017: International congress on connective tissue

14.03.2017 | Event News

ICTM Conference: Turbine Construction between Big Data and Additive Manufacturing

07.03.2017 | Event News

 
Latest News

Northern oceans pumped CO2 into the atmosphere

27.03.2017 | Earth Sciences

Fingerprint' technique spots frog populations at risk from pollution

27.03.2017 | Life Sciences

Big data approach to predict protein structure

27.03.2017 | Life Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>