Prof David Parker from Durham University’s Chemistry Department was working with experts from Glasgow University, and a team of international researchers, when they discovered dramatic changes in the way that light was emitted by optical probes during a series of experiments.
Light has energy and carries information and the researchers used the optical probes to measure the behaviour of light and its interaction with proteins abundant in human blood. The fortuitous discovery has led to the creation of a new type of probe for examining protein interactions that could be used for cellular imaging.
By tracking the way in which proteins bind, the experiments will aid understanding of the function of the most abundant protein in the body, serum albumin. In the future the technique could help to understand how drugs used in medicine interact with the major protein found in blood.
Prof Parker says: “It’s a new step in the development of optical probes in chemistry and in observing the interaction between medical drugs and proteins.”
The Durham University-led team looked at how light behaved when serum albumin was added to the probes and found that the emitted polarised light had interesting characteristics.
Chirality, or handedness, is a key concept in Nature. In molecular chemistry, it refers to the concept of a molecule having two mirror images that cannot be superimposed onto each other; these are called enantiomers and pairs of these can be designated as ‘right-‘ and ‘left-handed.’
Light can be thought of as being made up of two left and right handed components and this property can be measured. The research team used optical probes with hi-spatial resolution and precision to track protein interactions and to see how the light rotates and inverts when passed through the proteins.
Prof Parker says: “We have found a way to use the inherent chirality of light to examine the interaction at the molecular level between a probe (the optical probe, itself of one handedness) and serum albumin (also of one handedness: hence akin to a hand/glove interaction) - the most abundant protein in blood.”
Based on a chiral lanthanide complex, the probe emits circularly polarised light that inverts sign on protein binding; monitoring the emitted light allows researchers to follow the interaction between the complex and the protein.
Observing this luminescence is a way of studying the chirality of the system, explains Prof Parker: “The optical signal we observed carries information in its circular polarisation. It’s a tricky process. You have to get the light in and out of the cells but crucially, in terms of biology, it can be done using microscopes in the laboratory so it’s non-invasive.”
The researchers found that only one enantiomer of certain europium and terbium complexes bound selectively to a drug binding site of the protein serum albumin, and that the luminescence changed dramatically. Prof Parker says: “This is the first example of chiral inversion using an emissive probe in this way.”
The researchers have been seeking to develop responsive optical probes for a while and were delighted when they finally cracked it.
Prof Parker said: “We were genuinely surprised. The binding energy and kinetics have to be just right - we've been lucky. Potentially this technology could be used to track protein association in living cells in real time.”
Alex Thomas | alfa
Researchers uncover protein-based “cancer signature”
05.12.2016 | Universität Basel
The Nagoya Protocol Creates Disadvantages for Many Countries when Applied to Microorganisms
05.12.2016 | Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
Have you ever wondered how you see the world? Vision is about photons of light, which are packets of energy, interacting with the atoms or molecules in what...
A multi-institutional research collaboration has created a novel approach for fabricating three-dimensional micro-optics through the shape-defined formation of porous silicon (PSi), with broad impacts in integrated optoelectronics, imaging, and photovoltaics.
Working with colleagues at Stanford and The Dow Chemical Company, researchers at the University of Illinois at Urbana-Champaign fabricated 3-D birefringent...
In experiments with magnetic atoms conducted at extremely low temperatures, scientists have demonstrated a unique phase of matter: The atoms form a new type of quantum liquid or quantum droplet state. These so called quantum droplets may preserve their form in absence of external confinement because of quantum effects. The joint team of experimental physicists from Innsbruck and theoretical physicists from Hannover report on their findings in the journal Physical Review X.
“Our Quantum droplets are in the gas phase but they still drop like a rock,” explains experimental physicist Francesca Ferlaino when talking about the...
The Max Planck Institute for Physics (MPP) is opening up a new research field. A workshop from November 21 - 22, 2016 will mark the start of activities for an innovative axion experiment. Axions are still only purely hypothetical particles. Their detection could solve two fundamental problems in particle physics: What dark matter consists of and why it has not yet been possible to directly observe a CP violation for the strong interaction.
The “MADMAX” project is the MPP’s commitment to axion research. Axions are so far only a theoretical prediction and are difficult to detect: on the one hand,...
Broadband rotational spectroscopy unravels structural reshaping of isolated molecules in the gas phase to accommodate water
In two recent publications in the Journal of Chemical Physics and in the Journal of Physical Chemistry Letters, researchers around Melanie Schnell from the Max...
16.11.2016 | Event News
01.11.2016 | Event News
14.10.2016 | Event News
05.12.2016 | Power and Electrical Engineering
05.12.2016 | Materials Sciences
05.12.2016 | Power and Electrical Engineering