Salmonella is widely prevalent in the animal kingdom. The reason we do not suffer from severe intestinal infections very often is due to our body's defence system, which manages to digest invading bacteria.
This is why, generally speaking, a healthy human being will only fall ill if he consumes more than 100.000 salmonella bacteria via a contaminated food source, such as eggs or meat. An international team of researchers, led by Prof. Ivan Dikic from the Goethe University in Frankfurt has now found out how body cells recognise salmonella and render it harmless.
Understanding this process at a molecular level is crucial in identifying new targets for treatment. Tropical and sub-tropical countries in particular, where various sub-species of salmonella are common, are experiencing a rapid increase in resistance to antibiotics, with children at greatest risk.
Salmonella infection begins with bacteria entering the epithelial cells of the intestinal mucosa. To prevent them multiplying there, special cell organelles, called autophagosomes are activated. These encircle the invaders and then become absorbed in other organelles – lysosomes – that contain certain special digestive enzymes, which break down the bacteria into their constituent parts. But how exactly do the autophagosomes recognise salmonella? Prof. Ivan Dikic and his research group at the Biochemistry Institute II have now shed light on this mechanism.
As reported in a current article in the scientific journal "Science", the salmonella are marked as 'waste material' by the molecule ubiquitin. In order for the autophagosomes to become active, the marked bacteria have to bind to another molecule – LC3 – on the autophagosomal membrane. Here, the protein optineurin plays a key role, linking the marked Salmonella to the autophagosmal LC3, thereby setting off a process of selective autophagy. But optineurin becomes active as a link only after being chemically modified by an enzyme, (in this case it is phosphorylated by the protein kinase TBK1). "We suspect that phosphorylation acts as a regulated switch to trigger selective autophagy of bacteria but might also prove significant in other cargoes like protein aggregates or damaged mitochondria" explains Prof. Ivan Dikic, underlining the importance of these findings. It is thought that impaired autophagy processes may be implicated in, among other things, the development of cancer as well as neurodegenerative diseases.
In the area of infectious diseases, these findings are particularly relevant in view of the fact that gastrointestinal disease caused by Salmonella enterica has rapidly increased since the mid-1980s. In Germany, approx. 30,000 cases were reported to the health authorities in 1985, but by 2005 the figure has risen to 52,000. Worldwide, 94 million people fall ill each year with acute gastroenteritis, and 155,000 of these die. Typhoid, a disease also caused by Salmonella, affects 16 million people annually and mortality rates reach 200,000, with children in particular falling victim to the disease. Bacteria are becoming increasingly resistant to antibiotics so that the potential for treating disease is limited. Chloramphenicol, a formerly popular broad-spectrum antibiotic, is now ineffective, and even Fluoroquinolones, currently a commonly prescribed antibiotic, is proving inadequate in fighting bacteria. As co-author Prof. Dirk Bumann from the Biozentrum at Basel University puts it: "There is a pressing need to find new forms of treatment for infectious diseases. A better understanding of how the body's own defence mechanism makes use of autophagy will certainly help."
Publication: Philipp Wild et al: Phosphorylation of the Autophagy Receptor Optineurin restricts Salmonella growth, Science 26th May 2011 advanced online publication (Science DOI: 10.1126/science.1205405)
Novel mechanisms of action discovered for the skin cancer medication Imiquimod
21.10.2016 | Technische Universität München
Second research flight into zero gravity
21.10.2016 | Universität Zürich
Researchers from the Institute for Quantum Computing (IQC) at the University of Waterloo led the development of a new extensible wiring technique capable of controlling superconducting quantum bits, representing a significant step towards to the realization of a scalable quantum computer.
"The quantum socket is a wiring method that uses three-dimensional wires based on spring-loaded pins to address individual qubits," said Jeremy Béjanin, a PhD...
In a paper in Scientific Reports, a research team at Worcester Polytechnic Institute describes a novel light-activated phenomenon that could become the basis for applications as diverse as microscopic robotic grippers and more efficient solar cells.
A research team at Worcester Polytechnic Institute (WPI) has developed a revolutionary, light-activated semiconductor nanocomposite material that can be used...
By forcefully embedding two silicon atoms in a diamond matrix, Sandia researchers have demonstrated for the first time on a single chip all the components needed to create a quantum bridge to link quantum computers together.
"People have already built small quantum computers," says Sandia researcher Ryan Camacho. "Maybe the first useful one won't be a single giant quantum computer...
COMPAMED has become the leading international marketplace for suppliers of medical manufacturing. The trade fair, which takes place every November and is co-located to MEDICA in Dusseldorf, has been steadily growing over the past years and shows that medical technology remains a rapidly growing market.
In 2016, the joint pavilion by the IVAM Microtechnology Network, the Product Market “High-tech for Medical Devices”, will be located in Hall 8a again and will...
'Ferroelectric' materials can switch between different states of electrical polarization in response to an external electric field. This flexibility means they show promise for many applications, for example in electronic devices and computer memory. Current ferroelectric materials are highly valued for their thermal and chemical stability and rapid electro-mechanical responses, but creating a material that is scalable down to the tiny sizes needed for technologies like silicon-based semiconductors (Si-based CMOS) has proven challenging.
Now, Hiroshi Funakubo and co-workers at the Tokyo Institute of Technology, in collaboration with researchers across Japan, have conducted experiments to...
14.10.2016 | Event News
14.10.2016 | Event News
12.10.2016 | Event News
21.10.2016 | Health and Medicine
21.10.2016 | Information Technology
21.10.2016 | Materials Sciences