Scientists discover a non-destructive yet effective way to screen bats in hibernation for white-nose syndrome
Scientists working to understand the devastating bat disease known as white-nose syndrome (WNS) now have a new, non-lethal tool to identify bats with WNS lesions —ultraviolet, or UV, light.
If long-wave UV light is directed at the wings of bats with white-nose syndrome, it produces a distinctive orange-yellow fluorescence. This orange-yellow glow corresponds directly with microscopic skin lesions that are the current “gold standard” for diagnosing white-nose syndrome in bats.
“When we first saw this fluorescence of a bat wing in a cave, we knew we were on to something,” said Greg Turner from Pennsylvania Game Commission, who has been using this technique since 2010. “It was difficult to have to euthanize bats to diagnose WNS when the disease itself was killing so many. This was a way to get a good indication of which bats were infected and take a small biopsy for testing rather than sacrifice the whole bat.”
Millions of bats in the United States have died from the fungal disease called White nose syndrome which is caused by the fungus Pseudogymnoascus (Geomyces) destructans (Pd). White-nose syndrome was first seen in New York during the winter of 2006. Since then, the disease has spread to 25 US states and 5 Canadian provinces.
A significant problem in studying WNS has been the unreliability of visual onsite inspection when checking for WNS in bats during hibernation; the only way to confirm presence of disease was to euthanize the bats and send them back to a laboratory for testing.
“Ultraviolet light was first used in 1925 to look for ringworm fungal infections in humans,” said Carol Meteyer, USGS scientist and one of the lead authors on the paper. “The fact that this technique could be transferred to bats and have such remarkable precision for indicating lesions positive for Pd invasion is very exciting.”
To test the UV light’s effectiveness, bats with and without white-nose syndrome in North America were tested by the U.S. Geological Survey’s National Wildlife Health Center, first using UV light, then using traditional histological techniques to verify the UV light’s accuracy.
In the USGS lab testing, 98.8 percent of bats with the orange-yellow fluorescence tested positive for white-nose syndrome, whereas 100 percent of those that did not fluoresce tested negative for the disease. Targeted biopsies showed that pinpoint areas of fluorescence coincided with the microscopic wing lesions that are characteristic for WNS.
Researchers in the Czech Republic then tested the UV light-assisted biopsy technique in the field, using it to collect small samples from areas of bat wing that fluoresced under UV light. In this study, 95.5 percent of wing biopsies that targeted areas of fluorescence were microscopically positive for WNS lesions, while again 100 percent of bats that did not fluoresce were negative for WNS.
Combining research from two continents demonstrates that UV diagnostics might be applicable worldwide with great sensitivity and specificity in detecting WNS.
“Moreover, the technique hurts the animal minimally and bats fly away after providing data for research,” said Natalia Martinkova from the Academy of Sciences of the Czech Republic. “This makes UV fluorescence an ideal tool for studying endangered species.”
This effort included partners in the USFWS, state and federal biologists, the Czech Science Foundation, and the National Speleological Society of the USA.
This research article, “Nonlethal Screening of Bat-wing Skin with the Use of Ultraviolet Fluorescence to Detect Lesions Indicative of White-Nose Syndrome,” was recently published in the Journal of Wildlife Diseases. More information may be found on USGS research on white-nose syndrome here.
U.S. Department of the Interior, U.S. Geological Survey
Office of Communications and Publishing
12201 Sunrise Valley Dr, MS 119
Reston, VA 20192
Alex Demas | Eurek Alert!
Malaysia's unique freshwater mussels in danger
27.09.2016 | The University of Nottingham Malaysia Campus
How to detect water contamination in situ?
22.09.2016 | Tomsk Polytechnic University (TPU)
Friction stir welding is a still-young and thus often unfamiliar pressure welding process for joining flat components and semi-finished components made of light metals.
Scientists at the University of Stuttgart have now developed two new process variants that will considerably expand the areas of application for friction stir welding.
Technologie-Lizenz-Büro (TLB) GmbH supports the University of Stuttgart in patenting and marketing its innovations.
Friction stir welding is a still-young and thus often unfamiliar pressure welding process for joining flat components and semi-finished components made of...
Optical quantum computers can revolutionize computer technology. A team of researchers led by scientists from Münster University and KIT now succeeded in putting a quantum optical experimental set-up onto a chip. In doing so, they have met one of the requirements for making it possible to use photonic circuits for optical quantum computers.
Optical quantum computers are what people are pinning their hopes on for tomorrow’s computer technology – whether for tap-proof data encryption, ultrafast...
The Fraunhofer Institute for Organic Electronics, Electron Beam and Plasma Technology FEP has been developing various applications for OLED microdisplays based on organic semiconductors. By integrating the capabilities of an image sensor directly into the microdisplay, eye movements can be recorded by the smart glasses and utilized for guidance and control functions, as one example. The new design will be debuted at Augmented World Expo Europe (AWE) in Berlin at Booth B25, October 18th – 19th.
“Augmented-reality” and “wearables” have become terms we encounter almost daily. Both can make daily life a little simpler and provide valuable assistance for...
With the help of artificial intelligence, chemists from the University of Basel in Switzerland have computed the characteristics of about two million crystals made up of four chemical elements. The researchers were able to identify 90 previously unknown thermodynamically stable crystals that can be regarded as new materials. They report on their findings in the scientific journal Physical Review Letters.
Elpasolite is a glassy, transparent, shiny and soft mineral with a cubic crystal structure. First discovered in El Paso County (Colorado, USA), it can also be...
For the first time, Fraunhofer IKTS shows additively manufactured hardmetal tools at WorldPM 2016 in Hamburg. Mechanical, chemical as well as a high heat resistance and extreme hardness are required from tools that are used in mechanical and automotive engineering or in plastics and building materials industry. Researchers at the Fraunhofer Institute for Ceramic Technologies and Systems IKTS in Dresden managed the production of complex hardmetal tools via 3D printing in a quality that are in no way inferior to conventionally produced high-performance tools.
Fraunhofer IKTS counts decades of proven expertise in the development of hardmetals. To date, reliable cutting, drilling, pressing and stamping tools made of...
28.09.2016 | Event News
27.09.2016 | Event News
23.09.2016 | Event News
29.09.2016 | Physics and Astronomy
29.09.2016 | Earth Sciences
29.09.2016 | Physics and Astronomy