Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

The betrayal of the aphids

03.06.2014

UC Riverside-led research team shows how a bacterial protein in aphid saliva triggers plant defense against aphids

Aphids are devastating insect pests and cause great losses to agriculture worldwide. These sap-feeding plant pests harbor in their body cavity bacteria, which are essential for the aphids' fecundity and survival. Buchnera, the bacterium, benefits also because it cannot grow outside the aphid.

Aphids on Leaves

This photo shows aphids feeding on leaves.

Credit: Scott Edwards, UC Riverside


This photo shows aphids feeding on a stem.

Credit: Scott Edwards, UC Riverside.

This mutually beneficial relationship is sabotaged, however, by the bacterium which proceeds to betray the aphid, a research team led by scientists at the University of California, Riverside has found.

"Although this betrayal is unintentional, it nevertheless alerts the plant about the aphid's presence and the aphids are unable to reproduce in large numbers," said Isgouhi Kaloshian, a professor of nematology, who led the research project.

"A protein from the bacterium, found in the aphid saliva and likely delivered inside the plant host by the aphid, triggers plant immune responses against the aphid. It seems that the plant immune system targets the bacterium and exploits the strict mutual dependency between the plant and aphid to recognize the aphid as the intruder."

Study results appear online this week in the Proceedings of the National Academy of Sciences.

While feeding, aphids secrete saliva in the plant. To identify the protein composition of the aphid saliva, the researchers collected saliva from more than 100,000 aphids. Using mass spectrometry, they detected 105 proteins. They discovered these proteins were of both aphid and Buchnera origins. One of these Buchnera proteins, GroEL, was found to induce immune responses in plants.

"GroEL was known previously to trigger immunity in animals," said Kaloshian, a member of UC Riverside's Institute for Integrative Genome Biology. "However, our finding that it induces immunity in plants is new. Since most aphids harbor Buchnera, and likely have GroEL in their saliva, this bacterial protein may generally alert plants of the presence of aphids. How it is recognized by plants is still unknown. GroEL can now be exploited to engineer durable resistance of crops against aphids."

According to the researchers, since Buchnera-related bacteria are present in a number of insects (other than aphids), their findings are likely to be broadly applicable to other arthropods. GroEL and additional proteins from insect bacteria probably are delivered to plants through insect saliva and contribute to shaping plant-insect interactions.

"Strikingly, the majority of the aphid salivary proteins predicted for secretion were of unknown function and different from those typically secreted by microbes into plants," Kaloshian said. "However, these aphid salivary proteins, too, serve similar purposes in manipulating plant metabolism. Thus, aphids and microbes seem to have evolved different molecular solutions to achieve the same goals."

Currently, Kaloshian's lab is working on identifying the plant receptor for GroEL that initiates the plant immune response. Her team is also functionally characterizing the aphid salivary proteins with no known function to identify their roles.

"We would like to understand how these proteins are able to modulate host metabolism and identify their host targets," she said.

She was joined in the research by Ritu Chaudhary (first author of the research paper) and Hagop S. Atamian at UC Riverside; and Zhouxin Shen and Steven P. Briggs at UC San Diego. Shen and Briggs performed the mass spectrometry. The researchers used the model plant Arabidopsis in their experiments with the aphids.

###

The research was supported by a grant from the U.S. Department of Agriculture—National Institute of Food and Agriculture to Kaloshian and a grant from the National Science Foundation to Briggs.

The University of California, Riverside is a doctoral research university, a living laboratory for groundbreaking exploration of issues critical to Inland Southern California, the state and communities around the world. Reflecting California's diverse culture, UCR's enrollment has exceeded 21,000 students. The campus opened a medical school in 2013 and has reached the heart of the Coachella Valley by way of the UCR Palm Desert Center. The campus has an annual statewide economic impact of more than $1 billion. A broadcast studio with fiber cable to the AT&T Hollywood hub is available for live or taped interviews. UCR also has ISDN for radio interviews. To learn more, call (951) UCR-NEWS.

Iqbal Pittalwala | Eurek Alert!
Further information:
http://www.ucr.edu

Further reports about: Riverside bacteria bacterium function immune insect mass metabolism microbes proteins saliva salivary

More articles from Agricultural and Forestry Science:

nachricht Low-Altitude Aerial Images Allow Early Detection of Devastating Avocado Disease
28.05.2015 | University of Florida Institute of Food and Agricultural Sciences

nachricht New planning toolset gives farmers more options for improving water quality
28.05.2015 | American Society of Agronomy

All articles from Agricultural and Forestry Science >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Solid-state photonics goes extreme ultraviolet

Using ultrashort laser pulses, scientists in Max Planck Institute of Quantum Optics have demonstrated the emission of extreme ultraviolet radiation from thin dielectric films and have investigated the underlying mechanisms.

In 1961, only shortly after the invention of the first laser, scientists exposed silicon dioxide crystals (also known as quartz) to an intense ruby laser to...

Im Focus: Advance in regenerative medicine

The only professorship in Germany to date, one master's programme, one laboratory with worldwide unique equipment and the corresponding research results: The University of Würzburg is leading in the field of biofabrication.

Paul Dalton is presently the only professor of biofabrication in Germany. About a year ago, the Australian researcher relocated to the Würzburg department for...

Im Focus: Basel Physicists Develop Efficient Method of Signal Transmission from Nanocomponents

Physicists have developed an innovative method that could enable the efficient use of nanocomponents in electronic circuits. To achieve this, they have developed a layout in which a nanocomponent is connected to two electrical conductors, which uncouple the electrical signal in a highly efficient manner. The scientists at the Department of Physics and the Swiss Nanoscience Institute at the University of Basel have published their results in the scientific journal “Nature Communications” together with their colleagues from ETH Zurich.

Electronic components are becoming smaller and smaller. Components measuring just a few nanometers – the size of around ten atoms – are already being produced...

Im Focus: IoT-based Advanced Automobile Parking Navigation System

Development and implementation of an advanced automobile parking navigation platform for parking services

To fulfill the requirements of the industry, PolyU researchers developed the Advanced Automobile Parking Navigation Platform, which includes smart devices,...

Im Focus: First electrical car ferry in the world in operation in Norway now

  • Siemens delivers electric propulsion system and charging stations with lithium-ion batteries charged from hydro power
  • Ferry only uses 150 kilowatt hours (kWh) per route and reduces cost of fuel by 60 percent
  • Milestone on the road to operating emission-free ferries

The world's first electrical car and passenger ferry powered by batteries has entered service in Norway. The ferry only uses 150 kWh per route, which...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

International symposium: trends in spatial analysis and modelling for a more sustainable land use

20.05.2015 | Event News

15th conference of the International Association of Colloid and Interface Scientists

18.05.2015 | Event News

EHFG 2015: Securing health in Europe. Balancing priorities, sharing responsibilities

12.05.2015 | Event News

 
Latest News

Siemens will provide the first H-class power plant technology in Mexico

28.05.2015 | Press release

Merging galaxies break radio silence

28.05.2015 | Physics and Astronomy

A New Kind of Wood Chip: Collaboration Could Yield Biodegradable Computer Chips

28.05.2015 | Information Technology

VideoLinks
B2B-VideoLinks
More VideoLinks >>>