Electrical engineering professor Dr. Raimund Ober and his team recently published their findings in the journal Nature Methods. In the journal, they describe a method which minimizes the deterioration of images that can occur with conventional imaging approaches.
“Any image you take of an object is translated by the camera into pixels with added electronic noise,” Ober said. “Any distortion of an image makes it harder to obtain accurate estimates of the quantities you’re interested in.”
This method could greatly enhance the accuracy with which quantities of interest, such as the location, size, and orientation of an object, are extracted from the acquired images.
Ober and his team tackled this problem by using the EMCCD camera (a standard low-light image detector) in a highly unconventional setting. Using this method, scientists can estimate quantities of interest from the image data with substantially higher accuracy than those made with conventional low-light imaging.
“We have figured out through rigorous theoretical developments that when you run an EMCCD camera in such a way that very few photons hit each of its pixels, the resulting image is minimally corrupted by the camera noise,” he said. “Our method is about using the EMCCD camera to its fullest potential, beyond what is commonly believed to be possible by the scientific imaging community.”
By increasing the magnification of the image to reduce the number of photons detected in each image pixel, they were able to significantly reduce the camera noise and considerably lessen the deteriorative effect of pixilation.
In fact, the team managed to attain particle localization accuracy that was twofold higher than those obtained with conventional EMCCD imaging.
Ober and his team applied UAIM (Ultrahigh Accuracy Imaging Modality) to the live-cell tracking of a standard protein marker for breast cancer. By being able to accurately follow the movement of the marker, valuable insights on the biology of breast cancer could be gained.
“The tracking of individual proteins represents an important way to study cancer and other diseases at the molecular level,” Ober said. “The applications of UAIM for diagnostics and research are promising.”
The research team included Jerry Chao and Sripad Ram, post-doctoral researchers at UT Dallas, and Dr. Sally Ward, professor of immunology at UT Southwestern Medical Center.
The work was funded by the National Institutes of Health and the Cancer Prevention Research Institute of Texas.Media Contact: Katherine Morales, 972-883-4321, firstname.lastname@example.org
Katherine Morales | EurekAlert!
Reinforcement learning expedites 'tuning' of robotic prosthetics
18.01.2019 | North Carolina State University
Powerful microscope captures first image of nanoscaffold that promotes cell movement
14.01.2019 | Sanford Burnham Prebys Medical Discovery Institute
The scientific and political community alike stress the importance of German Antarctic research
Joint Press Release from the BMBF and AWI
The Antarctic is a frigid continent south of the Antarctic Circle, where researchers are the only inhabitants. Despite the hostile conditions, here the Alfred...
World first experiments on sensor that may revolutionise everything from medical devices to unmanned vehicles
The new sensor - capable of detecting vibrations of living cells - may revolutionise everything from medical devices to unmanned vehicles.
Dead and alive at the same time? Researchers at the Max Planck Institute of Quantum Optics have implemented Erwin Schrödinger’s paradoxical gedanken experiment employing an entangled atom-light state.
In 1935 Erwin Schrödinger formulated a thought experiment designed to capture the paradoxical nature of quantum physics. The crucial element of this gedanken...
Cellulose obtained from wood has amazing material properties. Empa researchers are now equipping the biodegradable material with additional functionalities to produce implants for cartilage diseases using 3D printing.
It all starts with an ear. Empa researcher Michael Hausmann removes the object shaped like a human ear from the 3D printer and explains:
The phenomenon of so-called superlubricity is known, but so far the explanation at the atomic level has been missing: for example, how does extremely low friction occur in bearings? Researchers from the Fraunhofer Institutes IWM and IWS jointly deciphered a universal mechanism of superlubricity for certain diamond-like carbon layers in combination with organic lubricants. Based on this knowledge, it is now possible to formulate design rules for supra lubricating layer-lubricant combinations. The results are presented in an article in Nature Communications, volume 10.
One of the most important prerequisites for sustainable and environmentally friendly mobility is minimizing friction. Research and industry have been dedicated...
16.01.2019 | Event News
14.01.2019 | Event News
12.12.2018 | Event News
18.01.2019 | Materials Sciences
18.01.2019 | Life Sciences
18.01.2019 | Health and Medicine