Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

New technology can detect anti-virus antibody in 20 minutes

25.05.2020

Researchers have succeeded in detecting anti-avian influenza virus antibody in blood serum within 20 minutes, using a portable analyzer they have developed to conduct rapid on-site bio tests. If a suitable reagent is developed, this technology could be used to detect antibodies against SARS-CoV-2, the causative virus of COVID-19.

Avian influenza is a poultry disease caused by influenza A virus infection. Rapid initial response for a suspected infection and continuous surveillance are essential to mitigate the damage from highly pathogenic, transmittable pathogens such as avian influenza viruses.


The microfluidic device to which ∼20 μL of samples containing 2 μL of serum will be applied. (Nishiyama K. et al., Sensors and Actuators B: Chemical. April 21, 2020)

Usage Restrictions: This image is copyrighted and can be used for reporting this research work without obtaining a permission if credited as instructed.

Generally, the polymerase chain reaction (PCR) method is used to detect the viral genome, but its complicated procedure requires a considerable amount of time. Another method involves detecting antibodies produced in the body in reaction to virus infection.

However, widely used antibody detection methods can be inaccurate because the antibodies' existence is generally determined by eyesight.

The group, including Keine Nishiyama, a doctoral student at Hokkaido University's Graduate School of Chemical Science and Engineering, and Professor Manabu Tokeshi of the university's Faculty of Engineering, conducted this study to develop a new method and analyzer capable of rapid, facile and selective detection of antibodies.

The method is based on conventional fluorescence polarization immunoassay (FPIA) but applies a different measurement mechanism to make the analyzer much smaller and portable. The analyzer weighs only 5.5 kilograms.

The combined use of liquid crystal molecules, an image sensor and the microfluidic device makes it possible to simultaneously examine multiple samples and reduces the volume of each sample required. Liquid crystal molecules are capable of controlling the polarization direction of fluorescent light, while the microfluidic device has a number of microchannels as a measurement vessel.

The group also developed a reagent to detect anti-H5 avian influenza virus antibody, a fluorescein-labeled protein that binds only with the antibody. The reagent was made by reproducing hemagglutinin (HA) protein fragments, which are expressed on the surface of H5 avian influenza virus, through gene recombination and by labeling fluorescent molecules to the fragments.

To make the measurement, serum collected from birds was mixed with the reagent and left for 15 minutes. The mixture was injected into the microfluidic device and measured with the portable fluorescence polarization analyzer. Molecular movements of the reagent bound with the antibody will be smaller in the liquid, producing a different degree of polarization from the reagent not bound with the antibody. The system can detect anti-H5 avian influenza virus antibody with only 2 microliters of serum sample and within 20 minutes.

"Our analyzer could be used to conduct other bio tests if suitable reagents are developed," says Tokeshi. The group has already successfully detected mycotoxin and drug constituents. "By reproducing fragments of spike proteins expressed in the novel coronavirus, and using them as the reagent, the analyzer should be able to detect anti-coronavirus antibodies."

Naoki Namba | EurekAlert!
Further information:
https://www.global.hokudai.ac.jp/blog/new-technology-can-detect-anti-virus-antibody-in-20-minutes/
http://dx.doi.org/10.1016/j.snb.2020.128160

More articles from Medical Engineering:

nachricht First COVID-19 Patient in Germany successfully treated with novel Diaphragm Therapy
10.07.2020 | Universität Greifswald

nachricht Restoring Vision Through Electrical Stimulation
09.07.2020 | Universität Zürich

All articles from Medical Engineering >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: The spin state story: Observation of the quantum spin liquid state in novel material

New insight into the spin behavior in an exotic state of matter puts us closer to next-generation spintronic devices

Aside from the deep understanding of the natural world that quantum physics theory offers, scientists worldwide are working tirelessly to bring forth a...

Im Focus: Excitation of robust materials

Kiel physics team observed extremely fast electronic changes in real time in a special material class

In physics, they are currently the subject of intensive research; in electronics, they could enable completely new functions. So-called topological materials...

Im Focus: Electrons in the fast lane

Solar cells based on perovskite compounds could soon make electricity generation from sunlight even more efficient and cheaper. The laboratory efficiency of these perovskite solar cells already exceeds that of the well-known silicon solar cells. An international team led by Stefan Weber from the Max Planck Institute for Polymer Research (MPI-P) in Mainz has found microscopic structures in perovskite crystals that can guide the charge transport in the solar cell. Clever alignment of these "electron highways" could make perovskite solar cells even more powerful.

Solar cells convert sunlight into electricity. During this process, the electrons of the material inside the cell absorb the energy of the light....

Im Focus: The lightest electromagnetic shielding material in the world

Empa researchers have succeeded in applying aerogels to microelectronics: Aerogels based on cellulose nanofibers can effectively shield electromagnetic radiation over a wide frequency range – and they are unrivalled in terms of weight.

Electric motors and electronic devices generate electromagnetic fields that sometimes have to be shielded in order not to affect neighboring electronic...

Im Focus: Gentle wall contact – the right scenario for a fusion power plant

Quasi-continuous power exhaust developed as a wall-friendly method on ASDEX Upgrade

A promising operating mode for the plasma of a future power plant has been developed at the ASDEX Upgrade fusion device at Max Planck Institute for Plasma...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

Contact Tracing Apps against COVID-19: German National Academy Leopoldina hosts international virtual panel discussion

07.07.2020 | Event News

International conference QuApps shows status quo of quantum technology

02.07.2020 | Event News

Dresden Nexus Conference 2020: Same Time, Virtual Format, Registration Opened

19.05.2020 | Event News

 
Latest News

X-ray scattering shines light on protein folding

10.07.2020 | Life Sciences

Looking at linkers helps to join the dots

10.07.2020 | Materials Sciences

Surprisingly many peculiar long introns found in brain genes

10.07.2020 | Life Sciences

VideoLinks
Science & Research
Overview of more VideoLinks >>>