Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Sisyphean movement of motor proteins may help preserve DNA integrity

27.10.2005


Researchers studying how proteins called helicases travel along strands of DNA have found that when the proteins hit an obstacle they snap back to where they began, repeating the process over and over, possibly playing a preventative role in keeping the genome intact.


A helicase (blue) moves rapidly on a highly flexible DNA track. Such movement may prevent the accumulation of toxic proteins on the DNA. Graphic courtesy Taekjip Ha



Taekjip Ha, a professor of physics at the University of Illinois at Urbana-Champaign and a Howard Hughes Medical Institute investigator, likens the biological scenario to Boston Red Sox baseball; the team rolls along only to hit a late-season obstacle called the New York Yankees. Then, like the always-anticipated annual cry from Chicago Cubs fan, it’s back to square one next year.

However, instead of causing more misery, as is the case for a baseball fan, this motor protein’s starting over may serve a beneficial purpose, clearing other, undesired proteins from the DNA, Ha said. The research was done in vitro, using purified proteins and studied with a technique that visualizes individual molecules on DNA. Whether the scenario plays out in real cells in not known and under exploration.


The discovery appears in the Oct. 27 issue of the journal Nature, along with a separate "News & Views" article by Eckhard Jankowsky, a biochemist at the Center for RNA Molecular Biology in Case Western University’s School of Medicine, who wrote about the potential importance of the findings.

Ha’s postdoctoral fellow Sua Myong led the study, looking at the Rep helicase from an E-coli bacterium. Rep is known to be involved in restarting DNA replication stalled by DNA damage. As a single protein, a monomer, Rep can travel one way along a single strand of DNA but by itself cannot unzip it. Rep’s progress was visualized using the single molecule fluorescence resonance energy transfer (FRET) technique that Ha had developed.

By tagging the protein and DNA with green and red dyes, Myong measured FRET changes as Rep traveled along single DNA strands, which are short segments extending out from double strands. Each time the protein reached either the junction of the full double-stranded DNA or hit an artificially created protein obstacle, Rep instantly returned to near the beginning of the single strand on which it had initially bound.

Upon closer examination using FRET, researchers discovered that Rep’s configuration gradually closed as it reached the obstacle in its path. Then, conformational changes of Rep allow it to grab and transfer to the end of the single-stranded DNA, leading to the next cycle.

"Although the very flexible single strand of DNA likely bombards the protein constantly, the protein doesn’t seem to pay attention to this overture until it hits a physical blockade," Ha said.

Researchers had theorized that obstacles would force motor proteins to disengage from DNA. "The finding was totally unexpected and may indicate a new function for the protein," Ha said. Jankowsky wrote that scientists "should not immediately search for the helix that the enzyme unzips, but instead remember how Rep snaps back."

In cells, single strands of DNA often occur when something is wrong, Ha said. The recycling action, he said, may represent a desirable function of the protein by keeping it engaged on a single strand, allowing time for repairs that allow normal DNA replication.

The human body has more than 200 types of helicases involved in replication, transcription, repair and other genetic processes, Ha said. Defective helicases have been linked to increased cancer risks and premature aging.

Co-authors with Myong and Ha were Ivan Rasnik, a former postdoctoral fellow who now is a professor of physics at Emory University in Atlanta; Chirlmin Joo, a doctoral student in Ha’s lab; and Timothy M. Lohman, a professor of biochemistry and molecular biophysics at the Washington University School of Medicine in St. Louis.

Jim Barlow | EurekAlert!
Further information:
http://www.uiuc.edu

More articles from Life Sciences:

nachricht Nanotubes built from protein crystals: Breakthrough in biomolecular engineering
15.11.2018 | Tokyo Institute of Technology

nachricht Insect Antibiotic Provides New Way to Eliminate Bacteria
15.11.2018 | Universität Zürich

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: A Chip with Blood Vessels

Biochips have been developed at TU Wien (Vienna), on which tissue can be produced and examined. This allows supplying the tissue with different substances in a very controlled way.

Cultivating human cells in the Petri dish is not a big challenge today. Producing artificial tissue, however, permeated by fine blood vessels, is a much more...

Im Focus: A Leap Into Quantum Technology

Faster and secure data communication: This is the goal of a new joint project involving physicists from the University of Würzburg. The German Federal Ministry of Education and Research funds the project with 14.8 million euro.

In our digital world data security and secure communication are becoming more and more important. Quantum communication is a promising approach to achieve...

Im Focus: Research icebreaker Polarstern begins the Antarctic season

What does it look like below the ice shelf of the calved massive iceberg A68?

On Saturday, 10 November 2018, the research icebreaker Polarstern will leave its homeport of Bremerhaven, bound for Cape Town, South Africa.

Im Focus: Penn engineers develop ultrathin, ultralight 'nanocardboard'

When choosing materials to make something, trade-offs need to be made between a host of properties, such as thickness, stiffness and weight. Depending on the application in question, finding just the right balance is the difference between success and failure

Now, a team of Penn Engineers has demonstrated a new material they call "nanocardboard," an ultrathin equivalent of corrugated paper cardboard. A square...

Im Focus: Coping with errors in the quantum age

Physicists at ETH Zurich demonstrate how errors that occur during the manipulation of quantum system can be monitored and corrected on the fly

The field of quantum computation has seen tremendous progress in recent years. Bit by bit, quantum devices start to challenge conventional computers, at least...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

“3rd Conference on Laser Polishing – LaP 2018” Attracts International Experts and Users

09.11.2018 | Event News

On the brain’s ability to find the right direction

06.11.2018 | Event News

European Space Talks: Weltraumschrott – eine Gefahr für die Gesellschaft?

23.10.2018 | Event News

 
Latest News

Massive impact crater from a kilometer-wide iron meteorite discovered in Greenland

15.11.2018 | Earth Sciences

When electric fields make spins swirl

15.11.2018 | Physics and Astronomy

Discovery of a cool super-Earth

15.11.2018 | Physics and Astronomy

VideoLinks
Science & Research
Overview of more VideoLinks >>>