The study is published in the January 24, 2010, advance online issue of the journal “Nature Cell Biology.”
“Having clear knowledge of embryonic development and how certain developmental processes can go awry is imperative for understanding the causes of the various types of human birth defects, which may eventually help us devise ways to correct those defects,” said Anthony Wynshaw-Boris, MD, PhD, chief of medical genetics at UCSF Children’s Hospital and a co-senior author of the study.
In the current study, the research team built upon previous work that uncovered the mechanism within the embryo responsible for specifying its left and right sides – a process called left-right symmetry breaking. That research, conducted by geneticist Hiroshi Hamada, MD, PhD, and colleagues at Japan’s Osaka University, showed how tail-like projections known as cilia located on the surface of cells in an embryonic region called the node generate a leftward flow of fluid outside the embryo, which, in turn, lets the embryo know which side is the left.
In this earlier work, Hamada’s group discovered that the cilia are able to produce a leftward flow of fluid because they are located toward the back of the node cells and are tilted toward the embryo’s tail end. This unique placement, coupled with the cilia’s clockwise circular beating motion, results in the leftward flow and, subsequently, the embryo’s left-right symmetry breaking. According to the researchers, if this directional flow is not established, organisms can develop with their internal organs on the wrong side of the body, decreasing chances for survival.
“Knowing that the cilia’s placement on the node is intricately involved with this key stage of embryonic development, we decided to take our work a step further to see whether certain genes might determine how cilia retain this tilted position,” said Hamada, who is also a co-senior author of the current study.
Hamada and Wynshaw-Boris decided to look at whether a specific gene family, called the “Dishevelled” gene family, might be directing the cilia’s migration to the back side of the node cells. Having researched this gene family for many years, the Wynshaw-Boris lab developed mouse models with each of the three Dishevelled genes “turned off” to study their individual functions. In doing so, they found that the Dishevelled genes activate a genetic pathway, called the planar cell polarity pathway, which helps determine positional information in cells and tissues.
“We focused on the Dishevelled gene family because from our previous work, we knew that these genes were involved in the development of hair cells within the inner ear of the embryo, and that the cilia-like structures at the edge of the hair cells behave in a similar fashion as those on the node of the embryo. That similarity made us take a closer look at how this gene family was acting on correct placement of the nodal cilia at this very early stage of development,” Wynshaw-Boris explained.
Masakazu Hashimoto, a graduate student in the Hamada lab and the first author of the study, monitored the movement of cilia in live mouse embryos using a high-speed camera attached to a microscope and observed that the cilia’s position actually changed as development proceeded. In the very earliest stages – before left-right symmetry breaking occurred – cilia were located in the center of the node cells; then, as development progressed, the cilia gradually moved to the back side of the cells.
The researchers compared cilia in normal mouse embryos to those in embryos with mutated versions of all three Dishevelled genes. They found that the cilia in the mutant embryos were misplaced on the node cells and therefore unable to produce a leftward flow of fluid.
“This discovery provides exciting information about how we are built the way we are at the most basic of levels: that is, how do we differentiate our left side from our right? Ultimately this determines how the heart ends up on the left side of the body and the liver on the right side, for example,” Wynshaw-Boris added.
Additional co-authors include Kyosuke Shinohara, Shingo Ikeuchi, Satoko Yoshiba, and Chikara Meno, all of Osaka University’s Developmental Genetics Group; Jianbo Wang of the University of California, San Diego, Department of Pediatrics and Medicine; Shigenori Nonaka of Japan’s National Institute for Basic Biology; Shinji Takada of the Okazaki Institute for Integrative Biosciences; and Kohei Hatta of the University of Hyogo Graduate School of Life Science.
The research was supported by a grant from Core Research for Evolutional Science and Technology of the Japan Science and Technology Corporation and a grant-in-aid from the Ministry of Education, Culture, Sports, Science, and Technology of Japan, as well as grants from the National Institutes of Health and March of Dimes.
UCSF Children’s Hospital creates an environment where children and their families find compassionate care at the forefront of scientific discovery, with more than 150 experts in 50 medical specialties serving patients throughout Northern California and beyond. The hospital admits about 5,000 children each year, including 2,000 babies born in the hospital. For more information, visit http:www.ucsfchildrenshospital.org.
UCSF is a leading university dedicated to promoting health worldwide through advanced biomedical research, graduate-level education in the life sciences and health professions, and excellence in patient care. For further information, visit http//www.ucsf.edu.
Researchers target protein that protects bacteria's DNA 'recipes'
21.08.2018 | University of Rochester
Protein interaction helps Yersinia cause disease
21.08.2018 | Schwedischer Forschungsrat - The Swedish Research Council
There are currently great hopes for solid-state batteries. They contain no liquid parts that could leak or catch fire. For this reason, they do not require cooling and are considered to be much safer, more reliable, and longer lasting than traditional lithium-ion batteries. Jülich scientists have now introduced a new concept that allows currents up to ten times greater during charging and discharging than previously described in the literature. The improvement was achieved by a “clever” choice of materials with a focus on consistently good compatibility. All components were made from phosphate compounds, which are well matched both chemically and mechanically.
The low current is considered one of the biggest hurdles in the development of solid-state batteries. It is the reason why the batteries take a relatively long...
New design tool automatically creates nanostructure 3D-print templates for user-given colors
Scientists present work at prestigious SIGGRAPH conference
Most of the objects we see are colored by pigments, but using pigments has disadvantages: such colors can fade, industrial pigments are often toxic, and...
Scientists at the University of California, Los Angeles present new research on a curious cosmic phenomenon known as "whistlers" -- very low frequency packets...
Scientists develop first tool to use machine learning methods to compute flow around interactively designable 3D objects. Tool will be presented at this year’s prestigious SIGGRAPH conference.
When engineers or designers want to test the aerodynamic properties of the newly designed shape of a car, airplane, or other object, they would normally model...
Researchers from TU Graz and their industry partners have unveiled a world first: the prototype of a robot-controlled, high-speed combined charging system (CCS) for electric vehicles that enables series charging of cars in various parking positions.
Global demand for electric vehicles is forecast to rise sharply: by 2025, the number of new vehicle registrations is expected to reach 25 million per year....
17.08.2018 | Event News
08.08.2018 | Event News
27.07.2018 | Event News
21.08.2018 | Ecology, The Environment and Conservation
21.08.2018 | Life Sciences
21.08.2018 | Power and Electrical Engineering