UC San Diego scientists explain how and when T cells become effector or memory lymphocytes
Using technologies and computational modeling that trace the destiny of single cells, researchers at the University of California, San Diego School of Medicine describe for the first time the earliest stages of fate determination among white blood cells called T lymphocytes, providing new insights that may help drug developers create more effective, longer-lasting vaccines against microbial pathogens or cancer.
This is the T lymphocyte.
Credit: National Institute of Allergy and Infectious Diseases
The findings are published in the March 2, 2014 online issue of Nature Immunology.
Naïve T lymphocytes patrol the front lines of the human body’s defense against infection, circulating in blood and tissues, searching for invasive microbes and other foreign antigens. They’re called “naïve” because they have not yet encountered an invader. When they do, these T cells activate and divide, giving rise to two types of daughter cells: “effector lymphocytes” responsible for immediate host defense and “memory lymphocytes” that provide long-term protection from similar infections.
“Researchers have been trying for a very long time to understand when and how T lymphocytes give rise to effector and memory cells during an infection,” said John T. Chang, MD, assistant professor in the Department of Medicine and the study’s co-principal investigator, along with Gene W. Yeo, PhD, assistant professor in the Department of Cellular and Molecular Medicine and Institute for Genomic Medicine.
However, all studies up to this point were based on analyses on bulk populations of cells, making it impossible to understand fate decisions made by individual cells. First authors Janilyn Arsenio, a postdoctoral fellow in the Chang lab and Boyko Kakaradov, a graduate student in the Yeo lab and UCSD Bioinformatics graduate program said that they took advantage of recent technological advances in single-cell gene expression profiling and cutting-edge machine-learning algorithms to address this question on a level of detail that was not previously possible.
Chang, Yeo and colleagues discovered that the decision by an individual T cell to produce effector and memory cells is made almost at the moment of infection. “The ‘mother’ lymphocyte seems to divide into two daughter cells that are already different from birth,” said Chang, “with one becoming an effector cell while its sister becomes a memory cell.”
Chang noted that the primary purpose of vaccines is to produce strong and durable immune protection, which depends heavily upon generation of memory lymphocytes. “Our work suggests that the way T lymphocytes divide early during a microbial infection might be critical to whether or not they give rise to long-lived memory cells. Strategies that improve this process could potentially enhance durable immunity and help us to design more effective vaccines.”
Co-authors of the study include Janilyn Arsenio, Patrick J. Metz and Stephanie H. Kim, UCSD Department of Medicine; Boyko Kakaradov, UCSD Department of Cellular and Molecular Medicine, UCSD Stem Cell and Bioinformatics programs and Institute for Genomic Medicine, UCSD; and Gene W. Yeo, UCSD Department of Cellular and Molecular Medicine, UCSD Stem Cell and Bioinformatics programs and Institute for Genomic Medicine, UCSD and National University of Singapore and Genome Institute of Singapore.
Funding for this research came, in part, from National Institutes of Health (grants DK080949, OD008469, AI095277, HG004659 and NS075449), UCSD Digestive Diseases Research Development Center, the California Institute for Regenerative Medicine, the National Science Foundation, the Alfred P. Sloan Foundation and the Howard Hughes Medical Institute.
Media contact: Scott LaFee, 619-543-6163, email@example.com
Scott LaFee | EurekAlert!
Penn study identifies viral product that promotes immune defense against RSV
04.09.2015 | University of Pennsylvania
Columbia Engineering team develops targeted drug delivery to lung
03.09.2015 | Columbia University School of Engineering and Applied Science
In a survey of NASA's Hubble Space Telescope images of 2,753 young, blue star clusters in the neighboring Andromeda galaxy (M31), astronomers have found that M31 and our own galaxy have a similar percentage of newborn stars based on mass.
By nailing down what percentage of stars have a particular mass within a cluster, or the Initial Mass Function (IMF), scientists can better interpret the light...
Researchers at the Fraunhofer Institute for Solar Energy Systems ISE have developed a highly compact and efficient inverter for use in uninterruptible power...
China's Loess Plateau was formed by wind alternately depositing dust or removing dust over the last 2.6 million years, according to a new report from University of Arizona geoscientists. The study is the first to explain how the steep-fronted plateau formed.
China's Loess Plateau was formed by wind alternately depositing dust or removing dust over the last 2.6 million years, according to a new report from...
The leaves of the lotus flower, and other natural surfaces that repel water and dirt, have been the model for many types of engineered liquid-repelling surfaces. As slippery as these surfaces are, however, tiny water droplets still stick to them. Now, Penn State researchers have developed nano/micro-textured, highly slippery surfaces able to outperform these naturally inspired coatings, particularly when the water is a vapor or tiny droplets.
Enhancing the mobility of liquid droplets on rough surfaces could improve condensation heat transfer for power-plant heat exchangers, create more efficient...
Longer, more severe, and hotter droughts and a myriad of other threats, including diseases and more extensive and severe wildfires, are threatening to transform some of the world's temperate forests, a new study published in Science has found. Without informed management, some forests could convert to shrublands or grasslands within the coming decades.
"While we have been trying to manage for resilience of 20th century conditions, we realize now that we must prepare for transformations and attempt to ease...
03.09.2015 | Event News
20.08.2015 | Event News
20.08.2015 | Event News
04.09.2015 | Power and Electrical Engineering
04.09.2015 | Machine Engineering
04.09.2015 | Materials Sciences