Based on a unique technology developed by A*STAR Singapore, these inventive and easy-to-use kits are versatile, effective and quick in the screening for modulators of protein-DNA interactions, as well as quality control (QC) analysis of transcription factor production.
Conventional techniques for the analysis of protein-DNA interactions tend to pose numerous restrictions and drawbacks upon their users in terms of assay time, expense and throughput. Some methods can involve extensive protocol optimization and materials preparation, with tedious procedures lasting as long as 2 days. Others necessitate tagging of the DNA with radioactive isotopes prior to running the test, which can result in safety and environmental issues. Label-free assays for academia or industry have typically involved hardware and software costs, which limit their utility for primary drug screening.
In comparison, users will find ColoQuik™ assays much easier to use. These tests require only a half-hour to complete on average, and allow for instant visual detection of reagent colour changes based on the quality and affinity of binding between a protein and a specified DNA sequence. The test does not require radioactive or fluorescent labeling of DNA which leads to significant time and cost savings on preparatory work. ColoQuik™ tests require only standard equipment such as microplate absorbance readers for semi-quantitative readouts. As such, these kits are amenable to high-throughput drug discovery applications and batch functional analysis of transcription factor production.
The key technology in the kits is the custom-made, nanometer-sized metal particles created at A*STAR’s IMRE. The particles act as probes which give off different colours depending on the specific protein-DNA combinations that are formed in the nanoparticle solution. “These kits offer the easiest and fastest ways to date for scientists to analyse a particular DNA binding protein for its impact on the gene transcription mechanism of specific diseases,” said Dr Su Xiaodi, the IMRE Senior Scientist who led the nanoparticle work.The Biofactory has received beneficial technology assessments from leading scientists who have utilized the technology for their specific experiments. The Biofactory is in the process of manufacturing and marketing the kits to research institutions and pharmaceutical laboratories both in Singapore and around the world.
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An international team of researchers has discovered a new anti-cancer protein. The protein, called LHPP, prevents the uncontrolled proliferation of cancer cells in the liver. The researchers led by Prof. Michael N. Hall from the Biozentrum, University of Basel, report in “Nature” that LHPP can also serve as a biomarker for the diagnosis and prognosis of liver cancer.
The incidence of liver cancer, also known as hepatocellular carcinoma, is steadily increasing. In the last twenty years, the number of cases has almost doubled...
In just a few weeks from now, the Chinese space station Tiangong-1 will re-enter the Earth's atmosphere where it will to a large extent burn up. It is possible that some debris will reach the Earth's surface. Tiangong-1 is orbiting the Earth uncontrolled at a speed of approx. 29,000 km/h.Currently the prognosis relating to the time of impact currently lies within a window of several days. The scientists at Fraunhofer FHR have already been monitoring Tiangong-1 for a number of weeks with their TIRA system, one of the most powerful space observation radars in the world, with a view to supporting the German Space Situational Awareness Center and the ESA with their re-entry forecasts.
Following the loss of radio contact with Tiangong-1 in 2016 and due to the low orbital height, it is now inevitable that the Chinese space station will...
Fraunhofer Institute for Organic Electronics, Electron Beam and Plasma Technology FEP, provider of research and development services for OLED lighting solutions, announces the founding of the “OLED Licht Forum” and presents latest OLED design and lighting solutions during light+building, from March 18th – 23rd, 2018 in Frankfurt a.M./Germany, at booth no. F91 in Hall 4.0.
They are united in their passion for OLED (organic light emitting diodes) lighting with all of its unique facets and application possibilities. Thus experts in...
A new scenario seeking to explain how Mars' putative oceans came and went over the last 4 billion years implies that the oceans formed several hundred million...
For the first time, an interdisciplinary team from the University of Basel has succeeded in integrating artificial organelles into the cells of live zebrafish embryos. This innovative approach using artificial organelles as cellular implants offers new potential in treating a range of diseases, as the authors report in an article published in Nature Communications.
In the cells of higher organisms, organelles such as the nucleus or mitochondria perform a range of complex functions necessary for life. In the networks of...
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