Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

University of the Basque Country researchers decode transcriptome for grey mullet

29.01.2010
The Cell Biology in Environmental Toxicology research team at the Department of Zoology and Animal Cell Biology at the University of the Basque Country (UPV/EHU) has decoded the transcriptome for the grey mullet. The director of the research project was Mr Ibon Cancio.

On more than one occasion we will have heard that the genome is the library where all the information of each organism is stored. This information is organised in various genes, where the information to synthesise the proteins that carry out most cell functions is stored.

The genome also has another type of genetic material that is not in the genes. This is the transcriptome - the part of the genome that is transcribed or is read. In most pluricellular beings it is usually more or less 1.5% of the genome.

This UPV/EHU research team has just decoded the transcriptome of the grey mullet. For a number of years now the researchers have been measuring the quality of river and sea water. For this it was necessary to have an animal capable of living in contaminated areas, one of which is the grey mullet. The aim was to measure the response that this animal has to contamination, in order to better know the quality of surrounding water. Besides, the grey mullet is very abundant in the rivers and sea of the Basque Country. Thus, according to Mr Cancio, it is the appropriate animal model, being very abundant and capable of surviving in contaminated areas.

More than half of the genes

The research was initiated in the Basque fishing port of Ondarroa, gathering a number of grey mullets: males, females, young fish, etc. Organs such as the liver, gills, gonads and brain were extirpated from each and the messenger RNA extracted. These samples of messenger RNA were suitably mixed to ensure that most of the transcriptome of the species would be found in the overall sample. Subsequently, the messenger RNA was converted to complementary DNA.

The samples of complementary DNA were sent to the sequencing department at the University of Newcastle in Britain. This university has a new sequencing system whereby, with just one analysis lasting seven and a half hours, 400,000 cDNA, can be sequenced, each with a length of 250 nucleotides. This was how the UPV/EHU research team obtained all the information about the transcriptome of the grey mullet; 126 million nucleotides, in concrete.

The most laborious task came later - making sense of all the information obtained, i.e. identifying the genes for each sequence, given that the function of the sequence can be found out from the gene. To this end, help from the General Research Services (SGIker) of the UPV/EHU was required.

Following this procedure, 18,332 genes were obtained. The aim was not to identify all the genes of the grey mullet, but more than half of them. With all this information a DNA microchip was developed in order to investigate the response of the mentioned genes to contamination.

For the upcoming year the challenge for the UPV/EHU research team to decode the transcriptome of the slug in order to generate a health profile of the soil.

Amaia Portugal | EurekAlert!
Further information:
http://www.elhuyar.com

Further reports about: Basque DNA RNA UPV/EHU cell death messenger RNA synthetic biology

More articles from Life Sciences:

nachricht A novel socio-ecological approach helps identifying suitable wolf habitats
17.02.2017 | Universität Zürich

nachricht New, ultra-flexible probes form reliable, scar-free integration with the brain
16.02.2017 | University of Texas at Austin

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Breakthrough with a chain of gold atoms

In the field of nanoscience, an international team of physicists with participants from Konstanz has achieved a breakthrough in understanding heat transport

In the field of nanoscience, an international team of physicists with participants from Konstanz has achieved a breakthrough in understanding heat transport

Im Focus: DNA repair: a new letter in the cell alphabet

Results reveal how discoveries may be hidden in scientific “blind spots”

Cells need to repair damaged DNA in our genes to prevent the development of cancer and other diseases. Our cells therefore activate and send “repair-proteins”...

Im Focus: Dresdner scientists print tomorrow’s world

The Fraunhofer IWS Dresden and Technische Universität Dresden inaugurated their jointly operated Center for Additive Manufacturing Dresden (AMCD) with a festive ceremony on February 7, 2017. Scientists from various disciplines perform research on materials, additive manufacturing processes and innovative technologies, which build up components in a layer by layer process. This technology opens up new horizons for component design and combinations of functions. For example during fabrication, electrical conductors and sensors are already able to be additively manufactured into components. They provide information about stress conditions of a product during operation.

The 3D-printing technology, or additive manufacturing as it is often called, has long made the step out of scientific research laboratories into industrial...

Im Focus: Mimicking nature's cellular architectures via 3-D printing

Research offers new level of control over the structure of 3-D printed materials

Nature does amazing things with limited design materials. Grass, for example, can support its own weight, resist strong wind loads, and recover after being...

Im Focus: Three Magnetic States for Each Hole

Nanometer-scale magnetic perforated grids could create new possibilities for computing. Together with international colleagues, scientists from the Helmholtz Zentrum Dresden-Rossendorf (HZDR) have shown how a cobalt grid can be reliably programmed at room temperature. In addition they discovered that for every hole ("antidot") three magnetic states can be configured. The results have been published in the journal "Scientific Reports".

Physicist Dr. Rantej Bali from the HZDR, together with scientists from Singapore and Australia, designed a special grid structure in a thin layer of cobalt in...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Booth and panel discussion – The Lindau Nobel Laureate Meetings at the AAAS 2017 Annual Meeting

13.02.2017 | Event News

Complex Loading versus Hidden Reserves

10.02.2017 | Event News

International Conference on Crystal Growth in Freiburg

09.02.2017 | Event News

 
Latest News

Biocompatible 3-D tracking system has potential to improve robot-assisted surgery

17.02.2017 | Medical Engineering

Real-time MRI analysis powered by supercomputers

17.02.2017 | Medical Engineering

Antibiotic effective against drug-resistant bacteria in pediatric skin infections

17.02.2017 | Health and Medicine

VideoLinks
B2B-VideoLinks
More VideoLinks >>>