Ribonucleic acid (RNA) is a biologically important molecule that is very similar to DNA, the blueprint of life. Naturally occurring RNAs, such as transfer RNA (tRNA), contain modified building blocks (“nucleosides”), which are involved in decoding genetic information.
Deazaguanosine nucleosides, in particular, are of significant interest for their antibacterial, antifungal, antiviral, and anticancer activity. In the European Journal of Organic Chemistry, Thomas Carell and his team at Munich's Ludwig Maximilians University (Germany) have now introduced a method to prepare tRNA nucleosides through a novel Turbo-Grignard-based approach with an unprecedented level of control from a common intermediate.
Because of the biological importance of deazaguanosines, a reliable method for their preparation is desirable. In this way, scientists can easily study their functions and the role they play in the treatment of diseases. One of the problems for synthetic chemists, however, is that these compounds often contain various reactive groups at several locations within the molecule. Precise control over the reactivity at a single position can therefore be difficult. Thus, the development of a site-specific reagent is required.
For their synthesis, the authors opted to use the versatile Turbo-Grignard reagent. The “normal” Grignard reagent is used by chemists to introduce a group into a molecule at a reactive site; it consists of the group to be added complexed to the metal magnesium. The Turbo-Grignard is also complexed to a lithium salt, which generally allows reactions to be performed under mild conditions – an important advantage when dealing with biologically relevant compounds.
In their article, the team shows that the Turbo-Grignard reagent has a specific point of attack and that it can be used in the presence of other reactive groups. Importantly, an adjacent group that proved problematic under different conditions was found to be completely unreactive to the turbo reagent, thereby allowing efficient synthesis of the desired nucleosides; the same reaction performed with the “normal” Grignard reagent resulted in decomposition of the desired products. The fact that other reactive groups in the molecule remain untouched facilitates the synthesis of deazaguanosine-derived tRNA nucleosides, which should enable detailed biochemical investigation of their functions in vivo and help in the treatment of genetic diseases.
Author: Thomas Carell, Ludwig-Maximilians-Universität München (Germany), http://www.cup.uni-muenchen.de/oc/carell/
Title: Efficient Synthesis of Deazaguanosine-Derived tRNA Nucleosides PreQ0, PreQ1, and Archaeosine Using the Turbo-Grignard Method
European Journal of Organic Chemistry, Permalink to the article: http://dx.doi.org/10.1002/ejoc.201000987
Scientists unlock ability to generate new sensory hair cells
22.02.2017 | Brigham and Women's Hospital
New insights into the information processing of motor neurons
22.02.2017 | Max Planck Florida Institute for Neuroscience
In the field of nanoscience, an international team of physicists with participants from Konstanz has achieved a breakthrough in understanding heat transport
Cells need to repair damaged DNA in our genes to prevent the development of cancer and other diseases. Our cells therefore activate and send “repair-proteins”...
The Fraunhofer IWS Dresden and Technische Universität Dresden inaugurated their jointly operated Center for Additive Manufacturing Dresden (AMCD) with a festive ceremony on February 7, 2017. Scientists from various disciplines perform research on materials, additive manufacturing processes and innovative technologies, which build up components in a layer by layer process. This technology opens up new horizons for component design and combinations of functions. For example during fabrication, electrical conductors and sensors are already able to be additively manufactured into components. They provide information about stress conditions of a product during operation.
The 3D-printing technology, or additive manufacturing as it is often called, has long made the step out of scientific research laboratories into industrial...
Nature does amazing things with limited design materials. Grass, for example, can support its own weight, resist strong wind loads, and recover after being...
Nanometer-scale magnetic perforated grids could create new possibilities for computing. Together with international colleagues, scientists from the Helmholtz Zentrum Dresden-Rossendorf (HZDR) have shown how a cobalt grid can be reliably programmed at room temperature. In addition they discovered that for every hole ("antidot") three magnetic states can be configured. The results have been published in the journal "Scientific Reports".
Physicist Dr. Rantej Bali from the HZDR, together with scientists from Singapore and Australia, designed a special grid structure in a thin layer of cobalt in...
13.02.2017 | Event News
10.02.2017 | Event News
09.02.2017 | Event News
22.02.2017 | Power and Electrical Engineering
22.02.2017 | Life Sciences
22.02.2017 | Physics and Astronomy