Their results are reported online in the October issue of the journal Stem Cells.
Acute lung injury (ALI) is responsible for an estimated 74,500 deaths in the U.S. each year. ALI can be caused by any major inflammation or injury to the lungs and is a major cause of death in patients in hospital ICUs. There is no effective drug treatment.
In ALI, the layer of cells that forms the lining of the blood vessels surrounding the lung's air sacs is damaged, allowing fluid to leak in and fill the sacs. Repair of these breaks in the endothelium, or lining, is complicated by the fact that endothelial cells are long-lived, says Kishore Wary, UIC assistant professor of pharmacology and lead author of the study. Turnover of new cells takes as long as two to five years, and few of the precursor cells needed for replacement circulate in the body at any given time.
"The stem cells that might be able repair the damage caused by ALI are simply not on hand," he said.
Wary and his colleagues were able to identify progenitor stem cells in the bone marrow of mice that could prevent and treat experimentally-induced ALI. These progenitor stem cells, named Flk-1 and CD34 for the proteins on their surfaces, constitute a very small percentage of the stem cell population in the bone marrow, but the researchers were able to develop a way of culturing the cells that increased their numbers and their "stickiness."
The stem cells stud their surface with molecules called integrins that allow the cells to stick to their targets and affect the repair. "Increasing this capacity for stickiness in our culture system was likely to make the stem cells more effective in repair," Wary said.
When mice that had been injected with a compound that causes ALI were injected with the purified and cultured Flk and CD34 stem cells, the progenitor cells were able to repair the lung injury, prevent fluid build-up, and led to improved survival.
The mouse disease model not only demonstrated that stem cell treatment is a promising therapy for ALI, Wary said, "but also provided us with the means to understand how these progenitor cells did their repair work. These therapeutic cells employed integrins to stick to the site of injury and turn on cellular and molecular repair machinery," he said.
The researchers hope to explore the possibility of using stem cell therapy in human acute lung injury.
The research was supported by grants from the National Institutes of Health. Stephen M. Vogel, Sean Garrean, Yidan D. Zhao and Asrar B. Malik, all of the department of pharmacology in the UIC College of Medicine, also contributed to the study.
Jeanne Galatzer-Levy | Newswise Science News
‘Farming’ bacteria to boost growth in the oceans
24.10.2016 | Max-Planck-Institut für marine Mikrobiologie
Calcium Induces Chronic Lung Infections
24.10.2016 | Universität Basel
Terahertz excitation of selected crystal vibrations leads to an effective magnetic field that drives coherent spin motion
Controlling functional properties by light is one of the grand goals in modern condensed matter physics and materials science. A new study now demonstrates how...
Researchers from the Institute for Quantum Computing (IQC) at the University of Waterloo led the development of a new extensible wiring technique capable of controlling superconducting quantum bits, representing a significant step towards to the realization of a scalable quantum computer.
"The quantum socket is a wiring method that uses three-dimensional wires based on spring-loaded pins to address individual qubits," said Jeremy Béjanin, a PhD...
In a paper in Scientific Reports, a research team at Worcester Polytechnic Institute describes a novel light-activated phenomenon that could become the basis for applications as diverse as microscopic robotic grippers and more efficient solar cells.
A research team at Worcester Polytechnic Institute (WPI) has developed a revolutionary, light-activated semiconductor nanocomposite material that can be used...
By forcefully embedding two silicon atoms in a diamond matrix, Sandia researchers have demonstrated for the first time on a single chip all the components needed to create a quantum bridge to link quantum computers together.
"People have already built small quantum computers," says Sandia researcher Ryan Camacho. "Maybe the first useful one won't be a single giant quantum computer...
COMPAMED has become the leading international marketplace for suppliers of medical manufacturing. The trade fair, which takes place every November and is co-located to MEDICA in Dusseldorf, has been steadily growing over the past years and shows that medical technology remains a rapidly growing market.
In 2016, the joint pavilion by the IVAM Microtechnology Network, the Product Market “High-tech for Medical Devices”, will be located in Hall 8a again and will...
14.10.2016 | Event News
14.10.2016 | Event News
12.10.2016 | Event News
24.10.2016 | Earth Sciences
24.10.2016 | Life Sciences
24.10.2016 | Physics and Astronomy