Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Scientists Propose A 'Genome Library' Of 10,000 Vertebrate Species

05.11.2009
In the most comprehensive study of animal evolution ever attempted, an international consortium of scientists plans to assemble a genomic library – a collection of DNA sequences for 10,000 vertebrate species, approximately one for every vertebrate genus.

Known as the Genome 10K Project, it involves gathering specimens of thousands of animals from zoos, museums, and university collections throughout the world, and then sequencing the genome of each species to reveal its complete genetic heritage.

Launched in April 2009 at a meeting at the University of California, Santa Cruz, the project now involves more than 68 scientists. Calling themselves the Genome 10K Community of Scientists, the group outlined its proposal to create a collection of tissue and DNA specimens for the project in a paper to be published online Nov. 5 in the Journal of Heredity.

The project was conceived by the paper’s three lead authors: David Haussler, professor of biomolecular engineering at UC Santa Cruz and a Howard Hughes Medical Institute investigator; Stephen J. O’Brien, chief of the Laboratory of Genomic Diversity at the National Cancer Institute; and Oliver A. Ryder, the director of genetics at the San Diego Zoo’s Institute for Conservation Research and adjunct professor of biology at UC San Diego.

“For the first time, we have a chance to really see evolution in action, caught in the act of changing whole genomes,” Haussler said. “This is possible because the technology to sequence DNA is thousands of times more powerful now than it was just a decade ago, and is poised to get even more powerful very soon.”

According to O’Brien, the cost of genome sequencing has been dropping steadily over the past decade, making the sequencing of 10,000 genomes a realistic possibility. “The original cost of sequencing the human genome by a major international consortium was over a billion dollars,” he said. “With the latest sequencing technology, it now costs $50,000 to $100,000 per genome. The price only needs to drop down another log or so to make the sequencing of 10,000 genomes possible.”

Among the authors is pioneering geneticist Sydney Brenner, a Nobel laureate and senior distinguished fellow at the Salk Institute. “The most challenging intellectual problem in biology for this century will be the reconstruction of our biological past so we can understand how complex organisms such as ourselves evolved,” Brenner said. “Genomes contain information from the past – they are molecular fossils – and having sequences from vertebrates will be an essential source of rich information.”

At the UCSC meeting, 55 leading scientists representing major zoos, museums, research centers, and universities around the world hashed out the challenging logistics involved in carrying out this ambitious project.

“These are scientists who have devoted their lives to biology, evolution, and the preservation of animals, and now they see an opportunity for deeper study,” Haussler said. “We came away from that meeting with a plan for moving forward and an extraordinary online database of samples from more than 16,000 different species of vertebrate animals compiled from more than 50 institutions.”

After recruiting a few more key scientists to broaden the depth of the collection, the participants collaborated on the database (available at http://sampledb.genome10k.org/) and drafted the proposal now published in the Journal of Heredity.

As part of the project, the genomic database will be analyzed to reveal the evolutionary changes it records, and it will be annotated with experimental findings related to specific sites of change. “Analysis of these data will be a far greater challenge than anything yet attempted in comparative genomics,” Haussler said.

O’Brien explained that the team hopes this project will integrate genomic inference into nearly every aspect of vertebrate biological enquiry.

“Biological science is about understanding how species work, so having the genomes available for 10,000 species will give us a new sense to understand biology,” he said. “The genome is like a sixth sense, adding to what we can see, smell, taste, hear, and feel. If we have this information for species that are not generally studied, it will be a particularly strong arrow in the quiver of students of biology.”

The scientists have identified specimens that span a broad range of evolutionary diversity. The species include living mammals, birds, reptiles, amphibians, and fishes, many of which are threatened or endangered, as well as some recently extinct species, O’Brien said.

“We are capturing what evolution left us with before the human population started impacting species – a set of genomes inclusive of the biota that a magnificent evolutionary process has produced,” he said.

Harris Lewin, a member of the Analysis Group and Director of the Institute for Genomic Biology at the University of Illinois, added that “for the first time we will be able to reconstruct ancestral chromosome organization at each of the major branch points of vertebrate evolution. This may help us to understand for the first time how chromosome organization relates to the major differences we see within and among the major vertebrate lineages. This has been the dream of the comparative genomics community since its inception about 30 years ago.”

Lewin served on two committees: one that planned how the data will be collected, stored and analyzed, and another dealing with sample collection from the various groups of mammals. Lewin’s lab has a longstanding research interest in the Artiodactyl group of mammals (even-toed ungulates), of which there are about 220 distinct species, including domesticated cattle and pigs.

Participants expect the Genome 10K Project to lay a foundation for understanding the genetic basis of recent and rapid adaptive changes within vertebrate species and between closely related species. The results can help conservation efforts by enabling scientists to predict how species will respond to climate change, pollution, emerging diseases, and invasive competitors.

“The risk of extinction is lessened for species for which we have a genome sequence, because it enables studies that can provide important information relevant to conservation,” Ryder said.

Genome sequences will be particularly valuable in efforts to assess genetic diversity in endangered populations. “Any tool used so far for evaluating genetic diversity and genetic variation is overshadowed by the resolving power of genomic information,” Ryder said.

The consortium agreed to a set of guidelines for sample collection, including the types and volumes of tissues, recommendations for preservation and documentation, and adherence to national and international statutes regulating the collection, use, and transport of biological specimens. Where possible, specimens for each species include both males and females and reflect geographic diversity or diversity within localized populations.

The collection will include more than a thousand frozen samples of fibroblast cells derived from 602 different vertebrate species. These cell samples, maintained by the San Diego Zoo, the National Cancer Institute, and the world’s cell repositories, are a valuable resource for genetic studies, according to Ryder.

“When you sequence a whole genome, it may be 3 billion bases, of which only a few percent code for genes. If you want to quickly learn something about the genes, you can sequence the RNA transcripts of the genes. These cells are robust sources of high-quality RNA,” Ryder said.

Because the evolution of species living today involved ancient genetic changes still preserved in their DNA, the Genome 10K project can help uncover answers to longstanding questions about the history of evolution. Having full genomes at hand will enable detailed studies of base-by-base evolutionary changes throughout the genome.

“Differences in the DNA that makes up the genomes of the animals we find today hold the key to the great biological events of the past, such as the development of the four-chambered heart and the magnificent architecture of wings, fins and arms, each adapted to its special purpose,” Haussler said.

But now the most challenging parts of the project begin, O’Brien said. “The first challenge is to bring this whole promise into reality by actually getting samples, characterizing them, doing quality control on them, and delivering them to sequencing centers that can accomplish the goal,” he said. “The second is to very quickly raise the money to pay for sequencing and analysis and annotation of the sequences.”

In addition to corresponding authors Haussler, O’Brien, and Ryder, co-authors of the paper who also served as committee chairs include F. Keith Barker of the University of Minnesota; Michele Clamp of the Broad Institute of MIT and Harvard; Andrew J. Crawford of Universidad de los Andes, Bogotá, Colombia; Robert Hanner of the Biodiversity Institute of Ontario, University of Guelph; Olivier Hanotte of the University of Nottingham; Warren E. Johnson of the National Cancer Institute, Laboratory of Genomic Diversity; Jimmy A. McGuire of the Museum of Vertebrate Zoology, University of California, Berkeley; Webb Miller of Pennsylvania State University; Robert W. Murphy of the Royal Ontario Museum, Toronto; and William J. Murphy of Texas A&M University.

Other co-authors: Frederick H. Sheldon of the Museum of Natural Science, Louisiana State University; Barry Sinervo of UC Santa Cruz; Byrappa Venkatesh of the Institute of Molecular and Cell Biology, Singapore; and Edward O. Wiley of the Natural History Museum and Biodiversity Research Center, University of Kansas.

The April 2009 Genome 10K meeting was supported in part by the American Genetic Association, Gordon and Betty Moore Foundation, NHGRI Intramural Sequencing Center, and UCSC Alumni Association. Individual researchers received funding from Howard Hughes Medical Institute; Gordon and Betty Moore Foundation; Assembling the Euteleost Tree of Life; National Science Foundation; The Global Viral Forecasting Initiative; Biomedical Research Council of A*STAR, Singapore; Natural Sciences and Engineering Research Council Discovery Grant; National Basic Research Program of China, the National Natural Science Foundation of China, and Bureau of Science and Technology of Yunnan Province; MCB SB RAS Programs; Portuguese-American Foundation for Development; CIBIO; UP; University of Montana; and Portuguese Science Foundation.

Harris Lewin | University of Illinois
Further information:
http://www.illinois.edu

More articles from Life Sciences:

nachricht The world's tiniest first responders
21.06.2018 | University of Southern California

nachricht A new toxin in Cholera bacteria discovered by scientists in Umeå
21.06.2018 | Schwedischer Forschungsrat - The Swedish Research Council

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Temperature-controlled fiber-optic light source with liquid core

In a recent publication in the renowned journal Optica, scientists of Leibniz-Institute of Photonic Technology (Leibniz IPHT) in Jena showed that they can accurately control the optical properties of liquid-core fiber lasers and therefore their spectral band width by temperature and pressure tuning.

Already last year, the researchers provided experimental proof of a new dynamic of hybrid solitons– temporally and spectrally stationary light waves resulting...

Im Focus: Overdosing on Calcium

Nano crystals impact stem cell fate during bone formation

Scientists from the University of Freiburg and the University of Basel identified a master regulator for bone regeneration. Prasad Shastri, Professor of...

Im Focus: AchemAsia 2019 will take place in Shanghai

Moving into its fourth decade, AchemAsia is setting out for new horizons: The International Expo and Innovation Forum for Sustainable Chemical Production will take place from 21-23 May 2019 in Shanghai, China. With an updated event profile, the eleventh edition focusses on topics that are especially relevant for the Chinese process industry, putting a strong emphasis on sustainability and innovation.

Founded in 1989 as a spin-off of ACHEMA to cater to the needs of China’s then developing industry, AchemAsia has since grown into a platform where the latest...

Im Focus: First real-time test of Li-Fi utilization for the industrial Internet of Things

The BMBF-funded OWICELLS project was successfully completed with a final presentation at the BMW plant in Munich. The presentation demonstrated a Li-Fi communication with a mobile robot, while the robot carried out usual production processes (welding, moving and testing parts) in a 5x5m² production cell. The robust, optical wireless transmission is based on spatial diversity; in other words, data is sent and received simultaneously by several LEDs and several photodiodes. The system can transmit data at more than 100 Mbit/s and five milliseconds latency.

Modern production technologies in the automobile industry must become more flexible in order to fulfil individual customer requirements.

Im Focus: Sharp images with flexible fibers

An international team of scientists has discovered a new way to transfer image information through multimodal fibers with almost no distortion - even if the fiber is bent. The results of the study, to which scientist from the Leibniz-Institute of Photonic Technology Jena (Leibniz IPHT) contributed, were published on 6thJune in the highly-cited journal Physical Review Letters.

Endoscopes allow doctors to see into a patient’s body like through a keyhole. Typically, the images are transmitted via a bundle of several hundreds of optical...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

Munich conference on asteroid detection, tracking and defense

13.06.2018 | Event News

2nd International Baltic Earth Conference in Denmark: “The Baltic Sea region in Transition”

08.06.2018 | Event News

ISEKI_Food 2018: Conference with Holistic View of Food Production

05.06.2018 | Event News

 
Latest News

Better model of water under extreme conditions could aid understanding of Earth's mantle

21.06.2018 | Earth Sciences

What are the effects of coral reef marine protected areas?

21.06.2018 | Life Sciences

The Janus head of the South Asian monsoon

21.06.2018 | Earth Sciences

VideoLinks
Science & Research
Overview of more VideoLinks >>>