The researchers describe, for the first time, that Merlin, the protein encoded within the NF2 gene interacts with a protein called angiomotin. This connection between Merlin and angiomotin also brings together two important information networks in cells, both of which have been implicated in numerous forms of cancer. It is a connection, the researchers say, between the sensors that detect interactions between cells and the signaling networks that drive cell division.
"Angiomotin is required for movement of cells that form new blood vessels, so it is fascinating to see it so closely linked to merlin, the product of the NF2 gene, loss of which leads to tumor formation," said Joseph Kissil, Ph.D., senior author of the study and associate professor in the Molecular and Cellular Oncogenesis Program of The Wistar Institute Cancer Center. "The discovery opens up a potential new method to treat NF2 by attacking the tumor cells directly and by starvation, a strategy already employed in certain cancer therapies."
"Drugs like Avastin, for example, target the growing blood vessels," Kissil said, "but what makes angiomotin a tempting target is that it is used by both blood vessels and the growing tumor cells that need the nutrients these blood vessels provide."
NF2 is a genetic disorder caused by a mutation in both copies of a person's NF2 gene. It occurs in about one in every 30,000 people, and it is mostly hereditary. NF2 generally appears as benign tumors in the nervous system of young adults, often causing deafness as tumors affect the auditory nerves. While the tumors are mostly benign, more malignant tumors may eventually arise. Moreover, even the benign tumors often cause debilitating pain as they spread throughout the nervous system. There is currently no treatment for NF2 other than surgery to remove tumors as they appear.
Mutations in the NF2 gene disrupt the function of the gene's protein product, Merlin, which is part of an elaborate molecular signaling pathway that regulates how cells grow and divide. These pathways are akin to information channels, and disrupting one protein can alter the function of other proteins both upstream and downstream along the channel. Merlin is particularly interesting to cancer biologists, as the mutations have been found in about half of all cases of the deadly lung cancer mesothelioma, and in some instances of thyroid, bladder and other cancers.
According to Kissil, Merlin normally stops cells from growing once they come into contact with adjacent cells. That is, Merlin binds to the angiomotin at "junctions," areas where cells come into contact with each other. When bound together, the interacting proteins relate a signal to the cell that, essentially, orders it to cease further growth and movement. It is a way for cells to coordinate their growth within a tissue. Cancerous cells, for example, often lack that sense of inhibition, and they will continue growing unchecked.
The Kissil laboratory plans to continue their exploration of angiomotin as a potential therapeutic target for treating NF2, as well as look into the role of angiomotin in other cancers known to be affected by NF2 mutations.
Funding for this study was provided in part through a grant to Kissil from the National Cancer Institute of the National Institutes of Health. The study was also supported by a Young Investigator Award from the Children's Tumor Foundation to Chunling Yi, Ph.D., a postdoctoral fellow in the Kissil laboratory.
Wistar collaborators also include research assistants Scott Troutman and Neepa Christian; graduate students Daniela Fera and Jacqueline L. Avila; and Wistar professors David W. Speicher, Ph.D. and Ronen Marmorstein, Ph.D. Co-authors also include Akihiko Shimono, Ph.D., of the Cancer Science Institute of Singapore at the National University of Singapore; Lars Holmgren, Ph.D., and Nathalie L. Persson of the Karolinska Institute in Stockholm, Sweden; and Anat Stemmer-Rachamimov, M.D., of Massachusetts General Hospital in Boston.
The Wistar Institute is an international leader in biomedical research with special expertise in cancer research and vaccine development. Founded in 1892 as the first independent nonprofit biomedical research institute in the country, Wistar has long held the prestigious Cancer Center designation from the National Cancer Institute. The Institute works actively to ensure that research advances move from the laboratory to the clinic as quickly as possible. The Wistar Institute: Today's Discoveries – Tomorrow's Cures.
Greg Lester | EurekAlert!
One step closer to reality
20.04.2018 | Max-Planck-Institut für Entwicklungsbiologie
The dark side of cichlid fish: from cannibal to caregiver
20.04.2018 | Veterinärmedizinische Universität Wien
University of Connecticut researchers have created a biodegradable composite made of silk fibers that can be used to repair broken load-bearing bones without the complications sometimes presented by other materials.
Repairing major load-bearing bones such as those in the leg can be a long and uncomfortable process.
Study published in the journal ACS Applied Materials & Interfaces is the outcome of an international effort that included teams from Dresden and Berlin in Germany, and the US.
Scientists at the Helmholtz-Zentrum Dresden-Rossendorf (HZDR) together with colleagues from the Helmholtz-Zentrum Berlin (HZB) and the University of Virginia...
Novel highly efficient and brilliant gamma-ray source: Based on model calculations, physicists of the Max PIanck Institute for Nuclear Physics in Heidelberg propose a novel method for an efficient high-brilliance gamma-ray source. A giant collimated gamma-ray pulse is generated from the interaction of a dense ultra-relativistic electron beam with a thin solid conductor. Energetic gamma-rays are copiously produced as the electron beam splits into filaments while propagating across the conductor. The resulting gamma-ray energy and flux enable novel experiments in nuclear and fundamental physics.
The typical wavelength of light interacting with an object of the microcosm scales with the size of this object. For atoms, this ranges from visible light to...
Stable joint cartilage can be produced from adult stem cells originating from bone marrow. This is made possible by inducing specific molecular processes occurring during embryonic cartilage formation, as researchers from the University and University Hospital of Basel report in the scientific journal PNAS.
Certain mesenchymal stem/stromal cells from the bone marrow of adults are considered extremely promising for skeletal tissue regeneration. These adult stem...
In the fight against cancer, scientists are developing new drugs to hit tumor cells at so far unused weak points. Such a “sore spot” is the protein complex...
13.04.2018 | Event News
12.04.2018 | Event News
09.04.2018 | Event News
20.04.2018 | Physics and Astronomy
20.04.2018 | Interdisciplinary Research
20.04.2018 | Physics and Astronomy