These findings will be presented for the first time at the American Heart Association’s (AHA) Scientific Sessions in Chicago Nov. 17.
The study, led by WenFeng Cai, PhD, a postdoctoral fellow under the direction of Litsa Kranias, PhD, AHA distinguished scientist and Hanna Chair in Cardiology in the department of pharmacology and cell biophysics, shows that a micro-RNA, known as miR765, which regulates gene expressions, can down-regulate the expression of protein phosphatase 1 inhibitor-1 (I-1) and reduce the contractility of cells that make up cardiac muscle.
"Previous studies have shown that the reduction in I-1 expression may play a role in the pathogenesis of heart disease,” Cai says. "However, the underlying molecular mechanism contributing to this down-regulation is unknown.
"We wanted to see if miR765 would serve as a candidate to regulate this protein expression and affect the contractility of the cardiac muscle cells.”
Using a gene transfer agent—or virus—researchers moved either miR-765 or a control agent into ventricular cells of animal models.
Data showed that the expression of I-1 messenger RNA was decreased by 20 percent in the miR765 cells of these models when compared with the control models.
"Under resting conditions, the contractile parameters were decreased in miR765-treated animal models,” Cai says. "Although beta adrenergic agonist, used to speed up the pumping action of the heart, had a positive effect, the contractile function remained suppressed in the miR765 group.”
Cai adds that analysis showed both phosphorylations of phospholamban and ryanodine receptor, the proteins that regulate calcium uptake and calcium release, were significantly reduced in the miR765 group both in the presence and in the absence of beta adrenergic agonist.
"These findings show that miR765 can down-regulate the expression and reduce contractility of heart cells by decreasing or deactivating a number of proteins that help the heart function at full capacity,” Cai says. "This leads us to believe that miR765 may play a role in the development of heart failure.
"Hopefully, these findings will lead to future studies, helping researchers and clinicians develop a therapeutic target to stop heart disease where it first starts: in the genes.”
This study was funded by the National Institutes of Health.
Katie Pence | EurekAlert!
Transport of molecular motors into cilia
28.03.2017 | Aarhus University
Asian dust providing key nutrients for California's giant sequoias
28.03.2017 | University of California - Riverside
The Institute of Semiconductor Technology and the Institute of Physical and Theoretical Chemistry, both members of the Laboratory for Emerging Nanometrology (LENA), at Technische Universität Braunschweig are partners in a new European research project entitled ChipScope, which aims to develop a completely new and extremely small optical microscope capable of observing the interior of living cells in real time. A consortium of 7 partners from 5 countries will tackle this issue with very ambitious objectives during a four-year research program.
To demonstrate the usefulness of this new scientific tool, at the end of the project the developed chip-sized microscope will be used to observe in real-time...
Astronomers from Bonn and Tautenburg in Thuringia (Germany) used the 100-m radio telescope at Effelsberg to observe several galaxy clusters. At the edges of these large accumulations of dark matter, stellar systems (galaxies), hot gas, and charged particles, they found magnetic fields that are exceptionally ordered over distances of many million light years. This makes them the most extended magnetic fields in the universe known so far.
The results will be published on March 22 in the journal „Astronomy & Astrophysics“.
Galaxy clusters are the largest gravitationally bound structures in the universe. With a typical extent of about 10 million light years, i.e. 100 times the...
Researchers at the Goethe University Frankfurt, together with partners from the University of Tübingen in Germany and Queen Mary University as well as Francis Crick Institute from London (UK) have developed a novel technology to decipher the secret ubiquitin code.
Ubiquitin is a small protein that can be linked to other cellular proteins, thereby controlling and modulating their functions. The attachment occurs in many...
In the eternal search for next generation high-efficiency solar cells and LEDs, scientists at Los Alamos National Laboratory and their partners are creating...
Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are less stable. Now researchers at the Technical University of Munich (TUM) have, for the first time ever, produced a composite material combining silicon nanosheets and a polymer that is both UV-resistant and easy to process. This brings the scientists a significant step closer to industrial applications like flexible displays and photosensors.
Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are...
20.03.2017 | Event News
14.03.2017 | Event News
07.03.2017 | Event News
29.03.2017 | Materials Sciences
29.03.2017 | Physics and Astronomy
29.03.2017 | Earth Sciences