The investigators created a large panel of novel mouse GAT1 transporters tagged with cyan or yellow fluorescent proteins (CFP and YFP) and optimized their expression in neuroblastoma cells. They determined the trafficking, subcellular localization, and oligomerization state of mGAT1 and correlated these features with transporter function.
One finding is that individual components of the FRET amplitude distribution reveal GAT1 dimers, high-order oligomers (likely tetramers), and oligomers associated via PDZ-mediated interactions with the actin cytoskeleton. Secondly, these details of the FRET amplitude distribution correlate with transporter function. Finally, the mGAT1 C-terminus PDZ-interacting domain is necessary for anchoring functional transporters to the actin cytoskeleton at the cell periphery; the corresponding FRET signal appears only in mGAT1 constructs with wild-type function. More generally, the results show the power of the FRET-based approach for distinguishing among distinct states of proteins.
About the Journal of General Physiology
Founded in 1918, the Journal of General Physiology (JGP) is published by the Rockefeller University Press. All editorial decisions on manuscripts submitted are made by active scientists. JGP content is posted to PubMed Central, where it is available to the public for free six months after publication. Authors retain copyright of their published works and third parties may reuse the content for non-commercial purposes under a creative commons license. For more information, please visit www.jgp.org.
Moss, F.J., et al. 2009. J. Gen. Physiol. doi:10.1085/jgp.200910314.
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