A new method of constructing artificial plant chromosomes from small rings of naturally occurring plant DNA can be used to transport multiple genes at once into embryonic plants where they are expressed, duplicated as plant cells divide, and passed on to the next generation -- a long-term goal for those interested in improving agricultural productivity.
In the October 19, 2007, issue of PLoS-Genetics, a team of academic and commercial researchers show that their "maize mini-chromosomes" (MMC) can introduce an entire "cassette" of novel genes into a plant in a way that is structurally stable and functional. Early results, the study authors say, "suggest that the MMC could be maintained indefinitely."
"This appears be the tool that agricultural scientists, and farmers, have long dreamed of," said Daphne Preuss, PhD, professor of molecular genetics and cell biology at the University of Chicago and chief scientific officer and president of Chromatin, Inc., the makers of the MMCs.
"This technology could be used to increase the hardiness, yield and nutritional content of crops," she said. "It could improve the production of ethanol or other biofuels. It could enable plants to make complex biochemicals, such as medicines, at very little expense."
It could also "cut one to two years out of any new transgenic project," said Preuss, who is taking a leave of absence from the University to bring this technology into the marketplace. "You get a better product faster, which saves time, reduces costs, and frees up resources."
The production of transgenic plants, including maize, has historically relied on techniques that integrate DNA fragments into a host chromosome. This can disrupt important native genes or lead to limited or unregulated expression of the added gene.
Currently, to add a single gene, plant scientists create hundreds of transgenic plants in which the new gene is randomly inserted into a plant chromosome. Then they screen the gene-altered plants to find the few that might be suitable for commercial use. If they want to add two genes, they create twice as many new plants, screen for single-gene successes, then cross breed them to get both new genes, a slow and laborious process.
Instead, Preuss and colleagues have constructed MMCs that contain DNA sequences found in maize centromeres, the chromosomal regions needed for inheritance. Rather than inserting the new genes randomly into a plant's natural chromosomes, these mini-chromosomes remain separate.
As a result, the new genes can be arranged in a defined sequence, with each gene surrounded by the desired regulatory mechanisms. This results in more consistent and controlled expression. The whole cassette of genes is passed on as a group during cell division as well as to the next generation.
In their PLoS paper, the researchers characterized the behavior of the maize mini-chromosome through four generations. Using a gene for red color as a marker, they showed that the added genes are expressed "in nearly every leaf cell, indicating stability through mitosis" -- the process in which a cell duplicates its chromosomes to generate two identical daughter cells.
They also show that the MMC is efficiently passed on through meiosis, the creation of gametes, to the next generation, at ratios "approaching Mendelian inheritance."
Taken together, the authors conclude, the maize mini-chromosome, once introduced, behaves much like an ordinary chromosome. It remains distinct from the other chromosomes. Its gene cassette is structurally stable from generation to generation. The genes it carries are expressed and it is transmitted through mitosis and meiosis.
This development has not gone unnoticed. Six years ago, Preuss and two of her post-doctoral students at the University, Gregory Copenhaver and Kevin Keith, started Chromatin to refine and apply this technology. On October 10, 2006, the United States Patent and Trademark Office issued Chromatin patent No. 7,119,250, which extends the exclusive right to use these mini-chromosomes to all plants. This includes "a crop plant," the patent states, "a commercial crop plant, a vegetable crop plant, a fruit and vine crop plant, a field crop plant."
On May 22, 2007, biotech giant Monsanto Company purchased non-exclusive rights to use Chromatin's mini-chromosome stacking technology in corn, cotton, soybeans, and canola. Chromatin is in discussions to license this technology to other companies, potentially capturing most of the US corn market.
The timing was ideal. The US, in order to limit oil imports and reduce greenhouse gasses, hopes to double its use of ethanol in fuels by 2012 and to double that twice over by 2022. Because of increased demand, corn prices rose this summer by about 50 percent over last year.
Preuss and colleagues hope to apply the technology to other plants, including sugar cane and switch grass, which could also serve as biofuel sources. They are also looking at other applications and expanding the gene carrying capacity of their mini-chromosomes. They have successfully delivered mini-chromosomes about six times the size of MMC1, suggesting that this platform can carry "a large number of genes."
John Easton | EurekAlert!
Molecular Force Sensors
20.09.2017 | Max-Planck-Institut für Biochemie
Foster tadpoles trigger parental instinct in poison frogs
20.09.2017 | Veterinärmedizinische Universität Wien
Whispering gallery mode (WGM) resonators are used to make tiny micro-lasers, sensors, switches, routers and other devices. These tiny structures rely on a...
Using ultrafast flashes of laser and x-ray radiation, scientists at the Max Planck Institute of Quantum Optics (Garching, Germany) took snapshots of the briefest electron motion inside a solid material to date. The electron motion lasted only 750 billionths of the billionth of a second before it fainted, setting a new record of human capability to capture ultrafast processes inside solids!
When x-rays shine onto solid materials or large molecules, an electron is pushed away from its original place near the nucleus of the atom, leaving a hole...
For the first time, physicists have successfully imaged spiral magnetic ordering in a multiferroic material. These materials are considered highly promising candidates for future data storage media. The researchers were able to prove their findings using unique quantum sensors that were developed at Basel University and that can analyze electromagnetic fields on the nanometer scale. The results – obtained by scientists from the University of Basel’s Department of Physics, the Swiss Nanoscience Institute, the University of Montpellier and several laboratories from University Paris-Saclay – were recently published in the journal Nature.
Multiferroics are materials that simultaneously react to electric and magnetic fields. These two properties are rarely found together, and their combined...
MBM ScienceBridge GmbH successfully negotiated a license agreement between University Medical Center Göttingen (UMG) and the biotech company Tissue Systems Holding GmbH about commercial use of a multi-well tissue plate for automated and reliable tissue engineering & drug testing.
MBM ScienceBridge GmbH successfully negotiated a license agreement between University Medical Center Göttingen (UMG) and the biotech company Tissue Systems...
Pathogenic bacteria are becoming resistant to common antibiotics to an ever increasing degree. One of the most difficult germs is Pseudomonas aeruginosa, a...
19.09.2017 | Event News
12.09.2017 | Event News
06.09.2017 | Event News
20.09.2017 | Life Sciences
20.09.2017 | Power and Electrical Engineering
20.09.2017 | Physics and Astronomy