Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Application of adult stem cells to regenerate hearts having suffered attacks by means of catheter

11.10.2007
A team of cardiologists at the University Hospital of Navarre, in collaboration with the Area of Cell Therapy at the same centre, and with the Gregorio Marañón Hospital in Madrid, have carried out clinical trials (phase II) on 50 patients in order to test the efficacy of adult stem sell transplants (in this case, myoblasts), in the heart of persons who have suffered a myocardial attack.

The great novelty of this work compared to others of a similar nature is the implantation of stem cells using a catheter and not through open surgery, used to date. The current study is based on previous research carried out on experimental animals. This is a study approved by the Spanish Medication Agency, an official body certifying that the research complies with the legal, ethical and safety requisites of the patient.

The patients that are or will be involved in this trial are those who have suffered myocardial arrest and have ventricular dysfunction. Before subjecting them to the stem cell transplant, a series of tests are carried out with them such as ecocardiograms, magnetic resonance, analysis and the evaluation of spontaneous arrhythmia using Holter-ECG.

The technique begins with the extraction of myoblast cells, by means of a biopsy of muscular tissue from the leg of the patient, a procedure carried out under local anaesthetic. From the tissue fragment obtained, the researchers isolate the adult muscle stem cells. These cellular units must be cultured for a month in order to obtain sufficient numbers of cells to carry out the transplant.

... more about:
»Adult »Myoblast »Stem »catheter »means »suffered »trial

The culture of the cells is undertaken in the GMP (Good Manufacturing Practice) laboratory of the University Hospital of Navarra, a technique that is based on using the patient’s own serum, unlike the cultures undertaken in other studies that use animal-origin serum – and thus reduces the risk of adverse reactions. “In this way, possible infections, allergies or immunological reactions are avoided, given that foreign proteins are not introduced”, according to doctor Juan José Gavira, cardiologist at the University Hospital of Navarra. The serum is obtained by carrying out a plasmapheresis, a technique which obtains the plasma in which the myoblasts are cultured.

After the month required for the growth of the cell population, the cells obtained are injected into the heart of the patient by catheterism, using a special injection catheter. The cells are implanted in and around the damaged areas of cardiac muscle.

One of the requirements included in the clinical trials that patients have to meet is that no other invasive therapy should simultaneously accompany this trial. In this way, pointed out Dr Gavira, “the results obtained are much purer, given that no other treatment interferes with them”. Having obtained the adult stem cells, a sample thereof is analysed in the Microbiology Laboratory in order to discard the existence of infections and to certify its perfect state.

The implant of stem cells

In order to implant myoblasts it is necessary to generate an anatomical reproduction of the left ventricle, which is the zone to be treated. This virtual reproduction is carried out by means of a navigation system known as non-fluoroscopic electroanatomical mapping. This technique manages to reconstruct the left ventricle in a three-dimensional form, a system that enables the location and analyses of low-voltage areas. “It is these zones without electrical activity that anatomically correspond to the heart attack. With this generation of the ventricle, obtained thanks to the navigation system, we identify the zone affected by the myocardial attack”, explains Doctor Ignacio García Bolao, specialist in the Cardiology Department at the University Hospital.

It is in this zone that the stem cells have to be injected. The procedure is undertaken by introducing a catheter made up of a very fine needle retractable at its end. The catheterism is carried out, as is habitual, through the femoral artery until the exact area of the heart affected is reached. Once there, between 15 and 20 injections of myoblasts are effected. The procedure is undertaken using local anaesthetic, with the patient being conscious, and lasts three or four hours. To date 14 patients have undergone the trials, all with satisfactory results.

The technique is part of research trials the Phase II goal of which is to see if this new treatment is effective and to improve on the cardiac function of patients who have suffered a heart attack.

Irati Kortabitarte | alfa
Further information:
http://www.basqueresearch.com/berria_irakurri.asp?Berri_Kod=1471&hizk=I

Further reports about: Adult Myoblast Stem catheter means suffered trial

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Making lightweight construction suitable for series production

More and more automobile companies are focusing on body parts made of carbon fiber reinforced plastics (CFRP). However, manufacturing and repair costs must be further reduced in order to make CFRP more economical in use. Together with the Volkswagen AG and five other partners in the project HolQueSt 3D, the Laser Zentrum Hannover e.V. (LZH) has developed laser processes for the automatic trimming, drilling and repair of three-dimensional components.

Automated manufacturing processes are the basis for ultimately establishing the series production of CFRP components. In the project HolQueSt 3D, the LZH has...

Im Focus: Wonder material? Novel nanotube structure strengthens thin films for flexible electronics

Reflecting the structure of composites found in nature and the ancient world, researchers at the University of Illinois at Urbana-Champaign have synthesized thin carbon nanotube (CNT) textiles that exhibit both high electrical conductivity and a level of toughness that is about fifty times higher than copper films, currently used in electronics.

"The structural robustness of thin metal films has significant importance for the reliable operation of smart skin and flexible electronics including...

Im Focus: Deep inside Galaxy M87

The nearby, giant radio galaxy M87 hosts a supermassive black hole (BH) and is well-known for its bright jet dominating the spectrum over ten orders of magnitude in frequency. Due to its proximity, jet prominence, and the large black hole mass, M87 is the best laboratory for investigating the formation, acceleration, and collimation of relativistic jets. A research team led by Silke Britzen from the Max Planck Institute for Radio Astronomy in Bonn, Germany, has found strong indication for turbulent processes connecting the accretion disk and the jet of that galaxy providing insights into the longstanding problem of the origin of astrophysical jets.

Supermassive black holes form some of the most enigmatic phenomena in astrophysics. Their enormous energy output is supposed to be generated by the...

Im Focus: A Quantum Low Pass for Photons

Physicists in Garching observe novel quantum effect that limits the number of emitted photons.

The probability to find a certain number of photons inside a laser pulse usually corresponds to a classical distribution of independent events, the so-called...

Im Focus: Microprocessors based on a layer of just three atoms

Microprocessors based on atomically thin materials hold the promise of the evolution of traditional processors as well as new applications in the field of flexible electronics. Now, a TU Wien research team led by Thomas Müller has made a breakthrough in this field as part of an ongoing research project.

Two-dimensional materials, or 2D materials for short, are extremely versatile, although – or often more precisely because – they are made up of just one or a...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Expert meeting “Health Business Connect” will connect international medical technology companies

20.04.2017 | Event News

Wenn der Computer das Gehirn austrickst

18.04.2017 | Event News

7th International Conference on Crystalline Silicon Photovoltaics in Freiburg on April 3-5, 2017

03.04.2017 | Event News

 
Latest News

DGIST develops 20 times faster biosensor

24.04.2017 | Physics and Astronomy

Nanoimprinted hyperlens array: Paving the way for practical super-resolution imaging

24.04.2017 | Materials Sciences

Atomic-level motion may drive bacteria's ability to evade immune system defenses

24.04.2017 | Life Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>