Increasingly, consumer products, especially food and beverage products, are being scrutinized for better quality. At the University of Missouri-Columbia, a food science expert has developed a rapid, reliable and efficient technique to ensure fruit and vegetable juice products adhere to federal and international quality standards.
Collaborating with scientists in the United States and from around the world, Mengshi Lin, assistant professor of food science in the College of Agriculture, Food and Natural Resources, has successfully used a new approach combining DNA sequencing technique with mid-infrared spectroscopy to rapidly and accurately identify Alicyclobacillus, a common bacterium found in apple, carrot, tomato, orange and pear juices, tropical fruit juices and juice blends. The bacterium won't cause human sickness, but it affects flavor and results in spoilage.
Currently, a number of different testing methods are utilized, some of which yield false negative results. This has complicated international trade. Japan, along with other developed countries, has a zero tolerance for this bacterium in imported juices, Lin said.
He said identification is a challenge because spoilage can be difficult to distinguish visibly until test results are confirmed or after juice products have been opened and tasted by consumers. In addition to agitating taste buds, the latter can affect consumer confidence.
Lin's technique is significant because it identifies the organism quickly - in a matter of hours, unlike traditional culturing methods, which are time consuming and require five to seven days to process. Lin said that testing time is critical for juice processing companies, which monitor for the bacteria during the processing and final product stage. He said the DNA technique in combination with infrared spectroscopy technique won't cause long delays in production.
"This combination will be the best way to quickly and accurately detect and identify the bacteria," said Lin, who worked with researchers from Washington State University and Hashemite University in Jordan, to develop the technique. "If processors find the bacteria, they can go back quickly and find the affected products."
Lin and his research team have tested the technique and published the results in a study, "Phylogenetic and spectroscopic analysis of Alicyclobacillus isolates by 16S rDNA sequencing and mid-infrared spectroscopy," which has been published in Sensing and Instrumentation for Food Quality and Safety.
Bryan Daniels | EurekAlert!
Molecular evolution: How the building blocks of life may form in space
26.04.2018 | American Institute of Physics
Multifunctional bacterial microswimmer able to deliver cargo and destroy itself
26.04.2018 | Max-Planck-Institut für Intelligente Systeme
Magnetic resonance imaging, or MRI, is a widely used medical tool for taking pictures of the insides of our body. One way to make MRI scans easier to read is...
At the Hannover Messe 2018, the Bundesanstalt für Materialforschung und-prüfung (BAM) will show how, in the future, astronauts could produce their own tools or spare parts in zero gravity using 3D printing. This will reduce, weight and transport costs for space missions. Visitors can experience the innovative additive manufacturing process live at the fair.
Powder-based additive manufacturing in zero gravity is the name of the project in which a component is produced by applying metallic powder layers and then...
Physicists at the Laboratory for Attosecond Physics, which is jointly run by Ludwig-Maximilians-Universität and the Max Planck Institute of Quantum Optics, have developed a high-power laser system that generates ultrashort pulses of light covering a large share of the mid-infrared spectrum. The researchers envisage a wide range of applications for the technology – in the early diagnosis of cancer, for instance.
Molecules are the building blocks of life. Like all other organisms, we are made of them. They control our biorhythm, and they can also reflect our state of...
University of Connecticut researchers have created a biodegradable composite made of silk fibers that can be used to repair broken load-bearing bones without the complications sometimes presented by other materials.
Repairing major load-bearing bones such as those in the leg can be a long and uncomfortable process.
Study published in the journal ACS Applied Materials & Interfaces is the outcome of an international effort that included teams from Dresden and Berlin in Germany, and the US.
Scientists at the Helmholtz-Zentrum Dresden-Rossendorf (HZDR) together with colleagues from the Helmholtz-Zentrum Berlin (HZB) and the University of Virginia...
13.04.2018 | Event News
12.04.2018 | Event News
09.04.2018 | Event News
26.04.2018 | Power and Electrical Engineering
26.04.2018 | Life Sciences
26.04.2018 | Power and Electrical Engineering