Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Protein sciences

12.03.2007
Expression and interaction of fluorescently labelled proteins makes living cells glow in different colours: A novel assay allows simultaneous detection of individual proteins and their interactions in living cells

Protein interactions direct cellular functions and their responses to pathogens and are important therapeutic targets. Scientists from the GSF Research Centre for Environment and Health have recently developed a method enabling simultaneous visualization of individual proteins and their interactions in living cells.

This is achieved by engineering the proteins to constantly emit red or blue fluorescent signals and to produce an additional yellow fluorescent signal upon interaction (see image below). Dr. Ruth Brack-Werner, Director of the GSF Institute of Molecular Virology (IMV) explains the decisive advantage of the new approach: “ In previous assays, signals were generated only by interacting proteins, whereas the individual partners remained undetected. However, the absence of signals could not be used to rule out protein interactions since the absence of one or both interaction partners would have the same effect. To overcome this problem Brack-Werner and her team developed the so-called extended bimolecular fluorescence complementation (exBiFC) which allows simultaneous monitoring of individual proteins and their interactions.

Dr. Ruth Brack-Werner, Institute of Molecular Virology of the GSF [300 dpi resolution for print] Photo: private.

... more about:
»Assay »Brack-Werner »HIV »REV »individual

Brack-Werner and her colleagues’ groundbreaking research work focusses on mechanisms that control replication of the human immunodeficiency virus (HIV), which causes AIDS. “HIV replication is based on the interaction of cellular proteins with viral proteins. Interactions involving viral regulatory factors have a direct impact on the amount of virus produced by the HIV host cell”, Brack-Werner explains. “Preventing HIV proteins from interacting with their crucial partners is a promising approach to developing novel therapies.” Therefore the GSF-scientists developed and validated exBiFC with the HIV Rev protein, which is an accelerator of HIV production. Various assays investigating Rev interactions in artificial settings indicate that the activity of Rev depends on the interaction of Rev molecules with each other and with cellular proteins. The latter include Exportin 1, which transports proteins from the nucleus to the cytoplasm and RISP, a modulator of HIV gene expression discovered by the Brack-Werner team in previous studies. Brack-Werner and her team demonstrated that exBIFC allows visualization of interactions of Rev with itself and with Exportin1 and RISP in living cells. In addition they were able to compare the strengths of the interactions of Rev with its partners by analysing the intensities of the signals in cell images.

ExBiFC has a wide range of potential appllications and represents an important tool for the elucidation of protein interaction networks and discovery of novel antiviral factors. Thus exBIFC has an enormous potential in the battle against leading global health problems such as infectious diseases and cancers.

GSF - Forschungszentrum für Umwelt und Gesundheit, Germany
Dept. of Public Affairs
Tel: 0049/89/3187-2460
Fax 0049/89/3187-3324
E-Mail: oea@gsf.de

Heinz Joerg Haury | EurekAlert!
Further information:
http://www.gsf.de

Further reports about: Assay Brack-Werner HIV REV individual

More articles from Life Sciences:

nachricht A Map of the Cell’s Power Station
18.08.2017 | Albert-Ludwigs-Universität Freiburg im Breisgau

nachricht On the way to developing a new active ingredient against chronic infections
18.08.2017 | Deutsches Zentrum für Infektionsforschung

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Fizzy soda water could be key to clean manufacture of flat wonder material: Graphene

Whether you call it effervescent, fizzy, or sparkling, carbonated water is making a comeback as a beverage. Aside from quenching thirst, researchers at the University of Illinois at Urbana-Champaign have discovered a new use for these "bubbly" concoctions that will have major impact on the manufacturer of the world's thinnest, flattest, and one most useful materials -- graphene.

As graphene's popularity grows as an advanced "wonder" material, the speed and quality at which it can be manufactured will be paramount. With that in mind,...

Im Focus: Exotic quantum states made from light: Physicists create optical “wells” for a super-photon

Physicists at the University of Bonn have managed to create optical hollows and more complex patterns into which the light of a Bose-Einstein condensate flows. The creation of such highly low-loss structures for light is a prerequisite for complex light circuits, such as for quantum information processing for a new generation of computers. The researchers are now presenting their results in the journal Nature Photonics.

Light particles (photons) occur as tiny, indivisible portions. Many thousands of these light portions can be merged to form a single super-photon if they are...

Im Focus: Circular RNA linked to brain function

For the first time, scientists have shown that circular RNA is linked to brain function. When a RNA molecule called Cdr1as was deleted from the genome of mice, the animals had problems filtering out unnecessary information – like patients suffering from neuropsychiatric disorders.

While hundreds of circular RNAs (circRNAs) are abundant in mammalian brains, one big question has remained unanswered: What are they actually good for? In the...

Im Focus: RAVAN CubeSat measures Earth's outgoing energy

An experimental small satellite has successfully collected and delivered data on a key measurement for predicting changes in Earth's climate.

The Radiometer Assessment using Vertically Aligned Nanotubes (RAVAN) CubeSat was launched into low-Earth orbit on Nov. 11, 2016, in order to test new...

Im Focus: Scientists shine new light on the “other high temperature superconductor”

A study led by scientists of the Max Planck Institute for the Structure and Dynamics of Matter (MPSD) at the Center for Free-Electron Laser Science in Hamburg presents evidence of the coexistence of superconductivity and “charge-density-waves” in compounds of the poorly-studied family of bismuthates. This observation opens up new perspectives for a deeper understanding of the phenomenon of high-temperature superconductivity, a topic which is at the core of condensed matter research since more than 30 years. The paper by Nicoletti et al has been published in the PNAS.

Since the beginning of the 20th century, superconductivity had been observed in some metals at temperatures only a few degrees above the absolute zero (minus...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Call for Papers – ICNFT 2018, 5th International Conference on New Forming Technology

16.08.2017 | Event News

Sustainability is the business model of tomorrow

04.08.2017 | Event News

Clash of Realities 2017: Registration now open. International Conference at TH Köln

26.07.2017 | Event News

 
Latest News

A Map of the Cell’s Power Station

18.08.2017 | Life Sciences

Engineering team images tiny quasicrystals as they form

18.08.2017 | Physics and Astronomy

Researchers printed graphene-like materials with inkjet

18.08.2017 | Materials Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>