Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Zebrafish Show Advantages in Assessing Human Cancer Cell-Induced Angiogenesis in vivo

10.08.2006
Researchers from Phylonix Pharmaceuticals, Inc., today announced results that demonstrates zebrafish is an efficient and effective animal model for assessing human cancer cells at various stages of tumorigenesis.

Results were published online today in the advanced online issue of the journal, Angiogenesis. Melanoma, colorectal and pancreatic cell lines proliferated, migrated, formed masses and stimulated angiogenesis in zebrafish. Cells were injected into various sites in zebrafish, including the yolk sac, the brain, and the circulation.

Significant findings of this research include: human cancer cells were not rejected by zebrafish embryos, a major problem with other animal models; new zebrafish angiogenic vessels formed in and around human cancer cell masses, similar to the process of cancer progression in humans; and zebrafish cells incorporated into human cell masses, indicating that cell signaling mechanisms are highly conserved.

The research demonstrates numerous advantages of using zebrafish as an alternative model for cancer research, such as: zebrafish are small, inexpensive to maintain, easily bred in large numbers, require as few as 50 cells for transplant, permit in vivo visualization of cell migration, mass formation and interaction between human cancer and zebrafish cells in the transparent animal, and require small amount of drug per experiment, µM vs mM.

Since chemicals can be delivered directly in the fish water and proteins can be injected, assessment of cytotoxic, apoptotic or anti-angiogenic effects of potential drug candidates, singly or in combination, is straightforward.

“Our research demonstrates that zebrafish provide important advantages as a research model, which will be beneficial for advancing cancer research and drug screening,” said Patricia McGrath, Phylonix’ President and Chief Executive Officer. “Although the mouse has been the model of choice for human cancer cell research and drug screening for more than 25 years, the time required to perform xenotransplant studies in mice ranges from several weeks to months, and it has been difficult to generate mouse models that exhibit tumor metastasis,” she continued.

A striking result of this research was formation of new angiogenic vessels at sites surrounding tumor mass formation which was confirmed by whole mount immunostaining and histology; activated zebrafish endothelial cells were embedded in the cancer cell masses.

The research, conducted by Maryann Haldi, Christopher Ton, Wen Lin Seng and Patricia McGrath, was supported by a grant from the National Institute for Diabetes and Digestive and Kidney Disease (DK074179).

For more information about this research or to request copies of the article and related images, please contact Phylonix at 617-444-6700, or visit www.phylonix.com.

About Phylonix

Phylonix, based in Cambridge, Massachusetts, is a privately owned biopharmaceutical research and services company that develops and markets zebrafish-based assays, eZ-Screens, used to assess safety and efficacy of drug candidates. The company owns several issued United States patents on critical research tools, including methods for drug screening in zebrafish using microtiter plates (6,299,858), transplantation of human cells in zebrafish (6,761,876), and methods for assessing drug candidates for angiogenesis and vasculargenesis in zebrafish (7,041,276). Phylonix’ drug candidate screening technologies and services are used by dozens of biotechnology and pharmaceutical customers world-wide.

James G. McManus | EurekAlert!
Further information:
http://www.phylonix.com

More articles from Life Sciences:

nachricht Immune Defense Without Collateral Damage
23.01.2017 | Universität Basel

nachricht The interactome of infected neural cells reveals new therapeutic targets for Zika
23.01.2017 | D'Or Institute for Research and Education

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Quantum optical sensor for the first time tested in space – with a laser system from Berlin

For the first time ever, a cloud of ultra-cold atoms has been successfully created in space on board of a sounding rocket. The MAIUS mission demonstrates that quantum optical sensors can be operated even in harsh environments like space – a prerequi-site for finding answers to the most challenging questions of fundamental physics and an important innovation driver for everyday applications.

According to Albert Einstein's Equivalence Principle, all bodies are accelerated at the same rate by the Earth's gravity, regardless of their properties. This...

Im Focus: Traffic jam in empty space

New success for Konstanz physicists in studying the quantum vacuum

An important step towards a completely new experimental access to quantum physics has been made at University of Konstanz. The team of scientists headed by...

Im Focus: How gut bacteria can make us ill

HZI researchers decipher infection mechanisms of Yersinia and immune responses of the host

Yersiniae cause severe intestinal infections. Studies using Yersinia pseudotuberculosis as a model organism aim to elucidate the infection mechanisms of these...

Im Focus: Interfacial Superconductivity: Magnetic and superconducting order revealed simultaneously

Researchers from the University of Hamburg in Germany, in collaboration with colleagues from the University of Aarhus in Denmark, have synthesized a new superconducting material by growing a few layers of an antiferromagnetic transition-metal chalcogenide on a bismuth-based topological insulator, both being non-superconducting materials.

While superconductivity and magnetism are generally believed to be mutually exclusive, surprisingly, in this new material, superconducting correlations...

Im Focus: Studying fundamental particles in materials

Laser-driving of semimetals allows creating novel quasiparticle states within condensed matter systems and switching between different states on ultrafast time scales

Studying properties of fundamental particles in condensed matter systems is a promising approach to quantum field theory. Quasiparticles offer the opportunity...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Sustainable Water use in Agriculture in Eastern Europe and Central Asia

19.01.2017 | Event News

12V, 48V, high-voltage – trends in E/E automotive architecture

10.01.2017 | Event News

2nd Conference on Non-Textual Information on 10 and 11 May 2017 in Hannover

09.01.2017 | Event News

 
Latest News

Tracking movement of immune cells identifies key first steps in inflammatory arthritis

23.01.2017 | Health and Medicine

Electrocatalysis can advance green transition

23.01.2017 | Physics and Astronomy

New technology for mass-production of complex molded composite components

23.01.2017 | Process Engineering

VideoLinks
B2B-VideoLinks
More VideoLinks >>>