Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Researchers devise way to mass-produce embryonic stem cells

16.03.2005


Ohio – Researchers at Ohio State University have developed a method for mass-producing embryonic stem cells.



That’s important because traditional laboratory methods used to grow these cells are costly and don’t produce cells fast enough to respond to increasing demands for human embryonic stem cells, said Shang-Tian Yang, a professor of chemical and biomolecular engineering at Ohio State University.

Federal rules forbid the federal funding of research on human embryonic stem cell lines that aren’t listed on the National Institutes of Health’s Human Embryonic Stem Cell Registry. There are currently 22 embryonic stem cell lines on the registry, and the demand for these cells is steadily growing. "We have to find a way to mass-produce them because traditional cell culturing methods can’t meet the projected high market demand for stem cells," Yang said.


He and Anli Ouyang, a doctoral student in chemical engineering, grew mouse embryonic stem cells in a bioreactor. Cell growth increased 193-fold in 15 days. At the end of that period, cell density – the number of cells that had grown in the bioreactor – was anywhere from 10- to 100-fold higher than the number of stem cells produced by conventional laboratory methods. That’s several hundreds of millions more stem cells.

Mass-producing cells like this could reduce stem cell production costs by at least 80 percent, Yang said, as it requires less equipment and monitoring. Embryonic stem cells are unspecialized, or undifferentiated, cells that can grow into any of the body’s 200 different types of cells. Yang and Ouyang presented their findings in San Diego on March 15 at the national meeting of the American Chemical Society.

They grew some mouse embryonic stem cells in a flask – a conventional way to grow stem cells – while other stem cells grew upon strands of polymer threads inside a bioreactor. The bioreactor used in this study is a tissue-growing device developed by Ohio State scientists. While this bioreactor could be used to produce adult stem cells, the researchers chose to look solely at embryonic stem cells for this experiment.

"There’s more of a demand for an unlimited supply of embryonic stem cells," Yang said. Also, embryonic stem cells are pluripotent – they can become any kind of cell in the body, while adult stem cells usually develop into a type of cell based on the kind of tissue that they originated from.

The bioreactor has a chamber that holds the polymer threads on which the stem cells grow and another chamber that holds fluid, or medium. This medium delivers chemical messengers, called cytokines, to the stem cells. The cytokines essentially tell the stem cells to stay in their undifferentiated state.

The difference between growing stem cells in a flask vs. the bioreactor is that the cells grown in the bioreactor could grow in three dimensions, while cells growing on a flat surface – the bottom of the flask – could not. “Cells grown on a flat surface don’t act like they would in the body," Yang said. "The growing surface affects how a cell forms, what it looks like and even how it expresses genes."

Cells grown in the bioreactor could grow for a much longer period of time than they could in the flask, as cells had more room to grow in the bioreactor. "In the same amount of time we could grow up to a billion stem cells per milliliter in the bioreactor, compared to tens of millions of cells per milliliter with conventional systems," Yang said.

Also, it seems that embryonic stem cells grown by conventional methods are more likely to spontaneously differentiate, or change. Researchers aren’t sure why or how some stem cells differentiate without prompting.

Yang and Ouyang tested both sets of cells – the ones grown in a flask and those grown in the bioreactor – for two key proteins. The presence of these proteins indicates that a stem cell has not differentiated. In the bioreactor experiment, 94 percent of the stem cells tested positive for these proteins, compared to about 85 percent of the cells grown in the flask.

The researchers are now working on ways to program embryonic stem cells so that they differentiate into specific types of cells. In preliminary work, Ouyang has created a network of neurons from undifferentiated embryonic stem cells.

The next step is to use human embryonic stem cells, and the researchers have yet to decide which line of stem cells to use.

The mouse embryonic stem cells used in this study were provided by ATCC, an organization that supplies lines of embryonic stem cell lines.

Shang-Tian Yang | EurekAlert!
Further information:
http://www.osu.edu

More articles from Life Sciences:

nachricht Repairing damaged hearts with self-healing heart cells
22.08.2017 | National University Health System

nachricht Biochemical 'fingerprints' reveal diabetes progression
22.08.2017 | Umea University

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Fizzy soda water could be key to clean manufacture of flat wonder material: Graphene

Whether you call it effervescent, fizzy, or sparkling, carbonated water is making a comeback as a beverage. Aside from quenching thirst, researchers at the University of Illinois at Urbana-Champaign have discovered a new use for these "bubbly" concoctions that will have major impact on the manufacturer of the world's thinnest, flattest, and one most useful materials -- graphene.

As graphene's popularity grows as an advanced "wonder" material, the speed and quality at which it can be manufactured will be paramount. With that in mind,...

Im Focus: Exotic quantum states made from light: Physicists create optical “wells” for a super-photon

Physicists at the University of Bonn have managed to create optical hollows and more complex patterns into which the light of a Bose-Einstein condensate flows. The creation of such highly low-loss structures for light is a prerequisite for complex light circuits, such as for quantum information processing for a new generation of computers. The researchers are now presenting their results in the journal Nature Photonics.

Light particles (photons) occur as tiny, indivisible portions. Many thousands of these light portions can be merged to form a single super-photon if they are...

Im Focus: Circular RNA linked to brain function

For the first time, scientists have shown that circular RNA is linked to brain function. When a RNA molecule called Cdr1as was deleted from the genome of mice, the animals had problems filtering out unnecessary information – like patients suffering from neuropsychiatric disorders.

While hundreds of circular RNAs (circRNAs) are abundant in mammalian brains, one big question has remained unanswered: What are they actually good for? In the...

Im Focus: RAVAN CubeSat measures Earth's outgoing energy

An experimental small satellite has successfully collected and delivered data on a key measurement for predicting changes in Earth's climate.

The Radiometer Assessment using Vertically Aligned Nanotubes (RAVAN) CubeSat was launched into low-Earth orbit on Nov. 11, 2016, in order to test new...

Im Focus: Scientists shine new light on the “other high temperature superconductor”

A study led by scientists of the Max Planck Institute for the Structure and Dynamics of Matter (MPSD) at the Center for Free-Electron Laser Science in Hamburg presents evidence of the coexistence of superconductivity and “charge-density-waves” in compounds of the poorly-studied family of bismuthates. This observation opens up new perspectives for a deeper understanding of the phenomenon of high-temperature superconductivity, a topic which is at the core of condensed matter research since more than 30 years. The paper by Nicoletti et al has been published in the PNAS.

Since the beginning of the 20th century, superconductivity had been observed in some metals at temperatures only a few degrees above the absolute zero (minus...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

Call for Papers – ICNFT 2018, 5th International Conference on New Forming Technology

16.08.2017 | Event News

Sustainability is the business model of tomorrow

04.08.2017 | Event News

Clash of Realities 2017: Registration now open. International Conference at TH Köln

26.07.2017 | Event News

 
Latest News

Cholesterol-lowering drugs may fight infectious disease

22.08.2017 | Health and Medicine

Meter-sized single-crystal graphene growth becomes possible

22.08.2017 | Materials Sciences

Repairing damaged hearts with self-healing heart cells

22.08.2017 | Life Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>