Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Wnt signalling protein Dishevelled acts in the nucleus, not just in the cytoplasm

15.02.2005


Researchers have identified that Dishevelled doesn’t only function in the cytoplasm and at the cell membrane – it must also pass into the nucleus. A study published today in Journal of Biology reveals that Dishevelled, a key player in the Wnt/beta-catenin signalling pathway, has to be localised in the nucleus to perform a key aspect of its function. This discovery should shed light on both normal embryonic development and the development of cancer.



In the paper, Sergei Sokol and colleagues, from Harvard Medical School, show that Dishevelled (Dsh) is constantly shuttling between the nucleus and the cytoplasm, owing to its nuclear export and import sequences, but that it has to be in the nucleus to respond to certain Wnt signals transmitted through the ‘canonical’ Wnt signalling pathway.

Dsh has always been considered to be a cytoplasmic protein, exerting its function as a stabiliser of beta-catenin in the cell cytoplasm after stimulation by Wnt secreted ligands binding to Frizzled receptors on the cell surface.


Sokol and colleagues show using Xenopus embryos and mammalian cultured cells that a mutated version of Dsh, which accumulates in the nucleus, is functional in the Wnt signalling pathway. Preventing Dsh from getting into the nucleus, however, either by mutating the nuclear localisation signal of the protein or by using a drug that disables the nuclear export machinery, impairs function. In mammalian cells, endogenous Dsh responds to Wnt ligands by mobilising to the nucleus.

“Our findings are consistent with a scenario in which Wnt signaling may cause nuclear translocation of Dsh followed by formation of a stable beta-catenin/Tcf3 complex and transcriptional activation of target genes”, explain the authors. Dsh’s exact role in the nucleus, however, is still unclear.

Dsh is also important in non-canonical Wnt signaling pathways, which involve proteins such as Rho GTPase and JNK. Sokol and colleagues show that nuclear localisation of Dsh is not required for its function in non-canonical signaling.

Juliette Savin | alfa
Further information:
http://www.biomedcentral.com

More articles from Life Sciences:

nachricht The birth of a new protein
20.10.2017 | University of Arizona

nachricht Building New Moss Factories
20.10.2017 | Albert-Ludwigs-Universität Freiburg im Breisgau

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Neutron star merger directly observed for the first time

University of Maryland researchers contribute to historic detection of gravitational waves and light created by event

On August 17, 2017, at 12:41:04 UTC, scientists made the first direct observation of a merger between two neutron stars--the dense, collapsed cores that remain...

Im Focus: Breaking: the first light from two neutron stars merging

Seven new papers describe the first-ever detection of light from a gravitational wave source. The event, caused by two neutron stars colliding and merging together, was dubbed GW170817 because it sent ripples through space-time that reached Earth on 2017 August 17. Around the world, hundreds of excited astronomers mobilized quickly and were able to observe the event using numerous telescopes, providing a wealth of new data.

Previous detections of gravitational waves have all involved the merger of two black holes, a feat that won the 2017 Nobel Prize in Physics earlier this month....

Im Focus: Smart sensors for efficient processes

Material defects in end products can quickly result in failures in many areas of industry, and have a massive impact on the safe use of their products. This is why, in the field of quality assurance, intelligent, nondestructive sensor systems play a key role. They allow testing components and parts in a rapid and cost-efficient manner without destroying the actual product or changing its surface. Experts from the Fraunhofer IZFP in Saarbrücken will be presenting two exhibits at the Blechexpo in Stuttgart from 7–10 November 2017 that allow fast, reliable, and automated characterization of materials and detection of defects (Hall 5, Booth 5306).

When quality testing uses time-consuming destructive test methods, it can result in enormous costs due to damaging or destroying the products. And given that...

Im Focus: Cold molecules on collision course

Using a new cooling technique MPQ scientists succeed at observing collisions in a dense beam of cold and slow dipolar molecules.

How do chemical reactions proceed at extremely low temperatures? The answer requires the investigation of molecular samples that are cold, dense, and slow at...

Im Focus: Shrinking the proton again!

Scientists from the Max Planck Institute of Quantum Optics, using high precision laser spectroscopy of atomic hydrogen, confirm the surprisingly small value of the proton radius determined from muonic hydrogen.

It was one of the breakthroughs of the year 2010: Laser spectroscopy of muonic hydrogen resulted in a value for the proton charge radius that was significantly...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

ASEAN Member States discuss the future role of renewable energy

17.10.2017 | Event News

World Health Summit 2017: International experts set the course for the future of Global Health

10.10.2017 | Event News

Climate Engineering Conference 2017 Opens in Berlin

10.10.2017 | Event News

 
Latest News

Terahertz spectroscopy goes nano

20.10.2017 | Information Technology

Strange but true: Turning a material upside down can sometimes make it softer

20.10.2017 | Materials Sciences

NRL clarifies valley polarization for electronic and optoelectronic technologies

20.10.2017 | Interdisciplinary Research

VideoLinks
B2B-VideoLinks
More VideoLinks >>>