Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:


ATP Determination Kits


In energy consuming biological reactions the level of ATP is the essential indicator for enzyme activity or cell viability. The Biaffin ATP Determination Kits offer convenient bioluminescense assays for quantitative determination of small amounts of pre-existing ATP or ATP formed in enzymatic reactions. Catalysed by firefly luciferase the substrate D-luciferin is oxidized in an ATP-dependent process generating chemiluminescence at 560 nm (pH 7.8):

The timestable assay is optimized for high throughput screening with nearly constant luminescence signals over a period of up to four hours. The sensitive assay can detect nanomolar to micromolar concentrations of ATP.

The sensitive assay is optimized for fast determination of low ATP levels. After a 10 min incubation of the assay reagent, ATP concentrations down to 0.1 pmol can be exactly determined using the linear luminescent signal of the luciferase reaction. Loss of luminescent signal and sensitivity is observed after incubation times of more than 30 minutes (for applications where longer incubation times are indispensable use the timestabe assay instead).

Both assays are available in packing units of 10 ml for 200 – 1000 assays or 100 ml sufficient for 2000 – 10000 assays.

The Kits consist of four components, which can be shipped at room temperature:
  • Firefly Luciferase (Component A, ready to use glycerol stock solution)
  • D-Luciferin (Component B, solid, to dissolve in reaction buffer)
  • Dithiothreitol DTT (Component C, solid, to dissolve in reaction buffer)
  • Reaction Buffer (Component D)

The final reaction mix can easily be prepared and stored light protected in appropriate aliquots at –20oC. The standard reaction for both assays can be performed by adding an equal volume of the final assay mix to the ATP solution (usually 10 to 50 µl) in a 96 or 384 well plate optimised for luminescent reading. The luminescent signal can be measured preferably in a luminometer after 10 minutes at room temperature. As an alternative to a luminometer a scintillation counter can be used to measure luciferase activity. Unknown ATP concentrations can be exactly and reproducible determined using a standard ATP concentration curve generated under comparable experimental conditions (temperature, incubation times, assay volume, luminometer adjustments, etc.)

More detailed information about the assay conditions, prices and order placement at

Biomolecular Interaction Analyses
Heinrich-Plett-Str. 40
D-34109 Kassel
Tel.: +49 (0) 561 804 4661
Fax: +49 (0) 561 804 4662

Dr. Stephan Drewianka | BIAFFIN GmbH & Co KG
Further information:

More articles from Life Sciences:

nachricht First time-lapse footage of cell activity during limb regeneration
25.10.2016 | eLife

nachricht Phenotype at the push of a button
25.10.2016 | Institut für Pflanzenbiochemie

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Etching Microstructures with Lasers

Ultrafast lasers have introduced new possibilities in engraving ultrafine structures, and scientists are now also investigating how to use them to etch microstructures into thin glass. There are possible applications in analytics (lab on a chip) and especially in electronics and the consumer sector, where great interest has been shown.

This new method was born of a surprising phenomenon: irradiating glass in a particular way with an ultrafast laser has the effect of making the glass up to a...

Im Focus: Light-driven atomic rotations excite magnetic waves

Terahertz excitation of selected crystal vibrations leads to an effective magnetic field that drives coherent spin motion

Controlling functional properties by light is one of the grand goals in modern condensed matter physics and materials science. A new study now demonstrates how...

Im Focus: New 3-D wiring technique brings scalable quantum computers closer to reality

Researchers from the Institute for Quantum Computing (IQC) at the University of Waterloo led the development of a new extensible wiring technique capable of controlling superconducting quantum bits, representing a significant step towards to the realization of a scalable quantum computer.

"The quantum socket is a wiring method that uses three-dimensional wires based on spring-loaded pins to address individual qubits," said Jeremy Béjanin, a PhD...

Im Focus: Scientists develop a semiconductor nanocomposite material that moves in response to light

In a paper in Scientific Reports, a research team at Worcester Polytechnic Institute describes a novel light-activated phenomenon that could become the basis for applications as diverse as microscopic robotic grippers and more efficient solar cells.

A research team at Worcester Polytechnic Institute (WPI) has developed a revolutionary, light-activated semiconductor nanocomposite material that can be used...

Im Focus: Diamonds aren't forever: Sandia, Harvard team create first quantum computer bridge

By forcefully embedding two silicon atoms in a diamond matrix, Sandia researchers have demonstrated for the first time on a single chip all the components needed to create a quantum bridge to link quantum computers together.

"People have already built small quantum computers," says Sandia researcher Ryan Camacho. "Maybe the first useful one won't be a single giant quantum computer...

All Focus news of the innovation-report >>>



Event News

#IC2S2: When Social Science meets Computer Science - GESIS will host the IC2S2 conference 2017

14.10.2016 | Event News

Agricultural Trade Developments and Potentials in Central Asia and the South Caucasus

14.10.2016 | Event News

World Health Summit – Day Three: A Call to Action

12.10.2016 | Event News

Latest News

Greater Range and Longer Lifetime

26.10.2016 | Power and Electrical Engineering

VDI presents International Bionic Award of the Schauenburg Foundation

26.10.2016 | Awards Funding

3-D-printed magnets

26.10.2016 | Power and Electrical Engineering

More VideoLinks >>>