Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

ATP Determination Kits

22.04.2004




In energy consuming biological reactions the level of ATP is the essential indicator for enzyme activity or cell viability. The Biaffin ATP Determination Kits offer convenient bioluminescense assays for quantitative determination of small amounts of pre-existing ATP or ATP formed in enzymatic reactions. Catalysed by firefly luciferase the substrate D-luciferin is oxidized in an ATP-dependent process generating chemiluminescence at 560 nm (pH 7.8):



The timestable assay is optimized for high throughput screening with nearly constant luminescence signals over a period of up to four hours. The sensitive assay can detect nanomolar to micromolar concentrations of ATP.



The sensitive assay is optimized for fast determination of low ATP levels. After a 10 min incubation of the assay reagent, ATP concentrations down to 0.1 pmol can be exactly determined using the linear luminescent signal of the luciferase reaction. Loss of luminescent signal and sensitivity is observed after incubation times of more than 30 minutes (for applications where longer incubation times are indispensable use the timestabe assay instead).

Both assays are available in packing units of 10 ml for 200 – 1000 assays or 100 ml sufficient for 2000 – 10000 assays.

The Kits consist of four components, which can be shipped at room temperature:
  • Firefly Luciferase (Component A, ready to use glycerol stock solution)
  • D-Luciferin (Component B, solid, to dissolve in reaction buffer)
  • Dithiothreitol DTT (Component C, solid, to dissolve in reaction buffer)
  • Reaction Buffer (Component D)

The final reaction mix can easily be prepared and stored light protected in appropriate aliquots at –20oC. The standard reaction for both assays can be performed by adding an equal volume of the final assay mix to the ATP solution (usually 10 to 50 µl) in a 96 or 384 well plate optimised for luminescent reading. The luminescent signal can be measured preferably in a luminometer after 10 minutes at room temperature. As an alternative to a luminometer a scintillation counter can be used to measure luciferase activity. Unknown ATP concentrations can be exactly and reproducible determined using a standard ATP concentration curve generated under comparable experimental conditions (temperature, incubation times, assay volume, luminometer adjustments, etc.)

More detailed information about the assay conditions, prices and order placement at www.proteinkinase.de/html/luciferin.html

BIAFFIN GmbH & Co KG
Biomolecular Interaction Analyses
Heinrich-Plett-Str. 40
D-34109 Kassel
Germany
Tel.: +49 (0) 561 804 4661
Fax: +49 (0) 561 804 4662
Mail: info@biaffin.com
www.biaffin.com
www.proteinkinase.de

Dr. Stephan Drewianka | BIAFFIN GmbH & Co KG
Further information:
http://www.proteinkinase.de/html/luciferin.html
http://www.proteinkinase.de
http://www.biaffin.com

More articles from Life Sciences:

nachricht New application for acoustics helps estimate marine life populations
16.01.2018 | University of California - San Diego

nachricht Unexpected environmental source of methane discovered
16.01.2018 | University of Washington Health Sciences/UW Medicine

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Scientists decipher key principle behind reaction of metalloenzymes

So-called pre-distorted states accelerate photochemical reactions too

What enables electrons to be transferred swiftly, for example during photosynthesis? An interdisciplinary team of researchers has worked out the details of how...

Im Focus: The first precise measurement of a single molecule's effective charge

For the first time, scientists have precisely measured the effective electrical charge of a single molecule in solution. This fundamental insight of an SNSF Professor could also pave the way for future medical diagnostics.

Electrical charge is one of the key properties that allows molecules to interact. Life itself depends on this phenomenon: many biological processes involve...

Im Focus: Paradigm shift in Paris: Encouraging an holistic view of laser machining

At the JEC World Composite Show in Paris in March 2018, the Fraunhofer Institute for Laser Technology ILT will be focusing on the latest trends and innovations in laser machining of composites. Among other things, researchers at the booth shared with the Aachen Center for Integrative Lightweight Production (AZL) will demonstrate how lasers can be used for joining, structuring, cutting and drilling composite materials.

No other industry has attracted as much public attention to composite materials as the automotive industry, which along with the aerospace industry is a driver...

Im Focus: Room-temperature multiferroic thin films and their properties

Scientists at Tokyo Institute of Technology (Tokyo Tech) and Tohoku University have developed high-quality GFO epitaxial films and systematically investigated their ferroelectric and ferromagnetic properties. They also demonstrated the room-temperature magnetocapacitance effects of these GFO thin films.

Multiferroic materials show magnetically driven ferroelectricity. They are attracting increasing attention because of their fascinating properties such as...

Im Focus: A thermometer for the oceans

Measurement of noble gases in Antarctic ice cores

The oceans are the largest global heat reservoir. As a result of man-made global warming, the temperature in the global climate system increases; around 90% of...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

10th International Symposium: “Advanced Battery Power – Kraftwerk Batterie” Münster, 10-11 April 2018

08.01.2018 | Event News

See, understand and experience the work of the future

11.12.2017 | Event News

Innovative strategies to tackle parasitic worms

08.12.2017 | Event News

 
Latest News

Gran Chaco: Biodiversity at High Risk

17.01.2018 | Ecology, The Environment and Conservation

Only an atom thick: Physicists succeed in measuring mechanical properties of 2D monolayer materials

17.01.2018 | Physics and Astronomy

Fraunhofer HHI receives AIS Technology Innovation Award 2018 for 3D Human Body Reconstruction

17.01.2018 | Awards Funding

VideoLinks
B2B-VideoLinks
More VideoLinks >>>