Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Foxd3 gene allows stem cells to remain stem cells

15.10.2002


Foxd3 joins the small, but growing list of stem cell regulating genes



In the search to understand the nature of stem cells, researchers at the University of Pennsylvania School of Medicine have identified a regulatory gene that is crucial in maintaining a stem cell’s ability to self-renew. According to their findings, the Foxd3 gene is a required factor for pluripotency – the ability of stem cells to turn into different types of tissue – in the mammalian embryo. Their research is presented in the October 15th issue of the journal Genes and Development.

"Stem cells represent a unique tissue type with great potential for disease therapy, but if we are to use stem cells then we ought to know the basis of their abilities," said Patricia Labosky, PhD, an Assistant Professor in the Department of Cell and Developmental Biology. "Among the stem cell regulatory genes, it appears that Foxd3 gene expression keeps stem cells from quickly differentiating – that is, developing into different types of tissue – holding back the process so that an embryo will have enough stem cells to continue developing normally."


To study the function of the Foxd3 gene, Labosky and her colleagues generated mice with an inactivating mutation in the gene, and then analyzed those mice to determine the role of the Foxd3 protein.

Foxd3-deficient embryos do not survive very long. While part of the yolk sac forms, the inner cell mass that contains all the cells that make up the body of the developing embryos fails to expand enough to produce the embryo and some of the supportive tissues. Without Foxd3, the mouse embryos simply could not maintain enough stem cells to survive a crucial point in their development.

"Our findings implicate Foxd3 as one of the few genes serving as a ’master switch’ of the developing embryo," said Labosky. "These genes determine the fate of cells by turning on or off other genes in response to signals in the embryo."

Foxd3 joins previously identified genes, such as Oct4, Fgf4, and Sox2, which control the pluripotency of embryonic stem cells in the early stages of embryogenesis. In their experiments, Labosky and her colleagues found that these genes are still expressed despite the lack of Foxd3. This suggests Foxd3 functions either downstream of Oct4, Fgf4 and Sox2, or along a parallel pathway.

The researchers determined that normal embryonic development can be restored by adding non-mutant embryonic stem cells to the Foxd3-mutant embryos, indicating that Foxd3 acts in the inner cell mass and its derivatives. According to Labosky, Foxd3 is a key regulator of mammalian stem cells, with a clear counterpart in humans. Foxd3 gene expression is a diagnostic characteristic of human embryonic stem cells, suggesting that the gene may function in a similar fashion in mouse and human cells.

"If we are to take advantage of stem cells as a clinical therapeutic, then it is absolutely vital to identify the key regulatory genes such as Foxd3 that control the process of cell differentiation," said Labosky. "Once we understand how these genes function we are that much closer to being able to mold stem cells to meet our needs."

Greg Lester | EurekAlert!
Further information:
http://www.med.upenn.edu/

More articles from Life Sciences:

nachricht Individual Receptors Caught at Work
19.10.2017 | Julius-Maximilians-Universität Würzburg

nachricht Rapid environmental change makes species more vulnerable to extinction
19.10.2017 | Universität Zürich

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Neutron star merger directly observed for the first time

University of Maryland researchers contribute to historic detection of gravitational waves and light created by event

On August 17, 2017, at 12:41:04 UTC, scientists made the first direct observation of a merger between two neutron stars--the dense, collapsed cores that remain...

Im Focus: Breaking: the first light from two neutron stars merging

Seven new papers describe the first-ever detection of light from a gravitational wave source. The event, caused by two neutron stars colliding and merging together, was dubbed GW170817 because it sent ripples through space-time that reached Earth on 2017 August 17. Around the world, hundreds of excited astronomers mobilized quickly and were able to observe the event using numerous telescopes, providing a wealth of new data.

Previous detections of gravitational waves have all involved the merger of two black holes, a feat that won the 2017 Nobel Prize in Physics earlier this month....

Im Focus: Smart sensors for efficient processes

Material defects in end products can quickly result in failures in many areas of industry, and have a massive impact on the safe use of their products. This is why, in the field of quality assurance, intelligent, nondestructive sensor systems play a key role. They allow testing components and parts in a rapid and cost-efficient manner without destroying the actual product or changing its surface. Experts from the Fraunhofer IZFP in Saarbrücken will be presenting two exhibits at the Blechexpo in Stuttgart from 7–10 November 2017 that allow fast, reliable, and automated characterization of materials and detection of defects (Hall 5, Booth 5306).

When quality testing uses time-consuming destructive test methods, it can result in enormous costs due to damaging or destroying the products. And given that...

Im Focus: Cold molecules on collision course

Using a new cooling technique MPQ scientists succeed at observing collisions in a dense beam of cold and slow dipolar molecules.

How do chemical reactions proceed at extremely low temperatures? The answer requires the investigation of molecular samples that are cold, dense, and slow at...

Im Focus: Shrinking the proton again!

Scientists from the Max Planck Institute of Quantum Optics, using high precision laser spectroscopy of atomic hydrogen, confirm the surprisingly small value of the proton radius determined from muonic hydrogen.

It was one of the breakthroughs of the year 2010: Laser spectroscopy of muonic hydrogen resulted in a value for the proton charge radius that was significantly...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

ASEAN Member States discuss the future role of renewable energy

17.10.2017 | Event News

World Health Summit 2017: International experts set the course for the future of Global Health

10.10.2017 | Event News

Climate Engineering Conference 2017 Opens in Berlin

10.10.2017 | Event News

 
Latest News

Electrode materials from the microwave oven

19.10.2017 | Materials Sciences

New material for digital memories of the future

19.10.2017 | Materials Sciences

Physics boosts artificial intelligence methods

19.10.2017 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>