GFP / RFP Dual color localization microscopy SPDMphymod / super resolution microscopy in a nucleus of a bone cancer cell: counting of 70,000 RFP-H2A-histone molecules & 50,000 GPF-Snf2H chromatin remodeling proteins (field of view of 470 µm², optical depth of 600 nm, each ‚spot‘ represents a single molecule, total 120000)
Foto: Prof. Christoph Cremer
The methodological simplicity of SPDMphymod technology is based on the fact that a single laser wavelength of suitable intensity is sufficient for nanoimaging the distribution of a given type of molecules, in contrast to other localization microscopy technologies which typically need two laser wavelengths in combination with especially designed photo-switchable/photo-activatable molecules and/or special chemical environments.
It is crucial for SPDMphymod that a single molecule is first transferred into a very long-living reversible dark state (with half-life of several seconds to minutes, i.e. orders of magnitude longer than typical ground state – triplet transitions), from which it returns to a fluorescent state, emitting many thousands of photons within several tens of milliseconds before returning into a very long-living so-called irreversible dark state.
Kaufmann R, Piontek J, Grüll F, Kirchgessner M, Rossa J, Wolburg H, Blasig IE and Cremer C (2012). Visualization and quantitative analysis of reconstituted tight junctions using localization microscopy. PLoS One, 7, e31128.
Dr. Regina Kratt | idw
Water forms 'spine of hydration' around DNA, group finds
26.05.2017 | Cornell University
How herpesviruses win the footrace against the immune system
26.05.2017 | Helmholtz-Zentrum für Infektionsforschung
Staphylococcus aureus is a feared pathogen (MRSA, multi-resistant S. aureus) due to frequent resistances against many antibiotics, especially in hospital infections. Researchers at the Paul-Ehrlich-Institut have identified immunological processes that prevent a successful immune response directed against the pathogenic agent. The delivery of bacterial proteins with RNA adjuvant or messenger RNA (mRNA) into immune cells allows the re-direction of the immune response towards an active defense against S. aureus. This could be of significant importance for the development of an effective vaccine. PLOS Pathogens has published these research results online on 25 May 2017.
Staphylococcus aureus (S. aureus) is a bacterium that colonizes by far more than half of the skin and the mucosa of adults, usually without causing infections....
Physicists from the University of Würzburg are capable of generating identical looking single light particles at the push of a button. Two new studies now demonstrate the potential this method holds.
The quantum computer has fuelled the imagination of scientists for decades: It is based on fundamentally different phenomena than a conventional computer....
An international team of physicists has monitored the scattering behaviour of electrons in a non-conducting material in real-time. Their insights could be beneficial for radiotherapy.
We can refer to electrons in non-conducting materials as ‘sluggish’. Typically, they remain fixed in a location, deep inside an atomic composite. It is hence...
Two-dimensional magnetic structures are regarded as a promising material for new types of data storage, since the magnetic properties of individual molecular building blocks can be investigated and modified. For the first time, researchers have now produced a wafer-thin ferrimagnet, in which molecules with different magnetic centers arrange themselves on a gold surface to form a checkerboard pattern. Scientists at the Swiss Nanoscience Institute at the University of Basel and the Paul Scherrer Institute published their findings in the journal Nature Communications.
Ferrimagnets are composed of two centers which are magnetized at different strengths and point in opposing directions. Two-dimensional, quasi-flat ferrimagnets...
An Australian-Chinese research team has created the world's thinnest hologram, paving the way towards the integration of 3D holography into everyday...
24.05.2017 | Event News
23.05.2017 | Event News
22.05.2017 | Event News
26.05.2017 | Life Sciences
26.05.2017 | Life Sciences
26.05.2017 | Physics and Astronomy