Scientists at the Reference- and Translation Center for Cardiac Stem Cell Therapy at the University of Rostock (RTC) are now able to visualize molecules with a microscope system with a localization accuracy of up to 10 nm.
Legend: Evgenya Delyagina, researcher at the RTC Rostock, will examine genetically modified stem cells with the help of the new microscope ELYRA PS.1. Photo: © Zeiss/RTC
This is achieved with a new product from Carl Zeiss MicroImaging GmbH, the microscope ELYRA PS.1. Besides Osaka the RTC Rostock is going to be the first user worldwide of a series system based on this technology. The project is funded by the Federal Ministry of Education and Research (BMBF).
„Our research deals with the efficacy, safety, and the further development of stem cell therapies against heart diseases. ELYRA will support our research “ Prof. Gustav Steinhoff, head of the RTC Rostock, explains. This microscope offers new interesting possibilities, especially for gene-technical and nano-technological approaches with stem cells. To improve the therapeutical efficacy or the survivability after transplantation the cells are genetically modified by using gene transfer systems. The gene transfer is currently conducted with a novel method whose mechanism has not been fully understood yet. Neither, it is known whether the modified cells are exposed to unexpected side effects.
With ELYRA PS.1, cell processes in live cells can now be observed and documented on a molecular level for the first time. Before that, it had been possible to image cellular structures with electron microscopy with a spatial resolution of up to 10 nanometers; however, living cells were destroyed in the course of the imaging procedure.
Combining two innovative technologies, SR-SIM und PALM, the ELYRA PS.1 is able to provide super-resolution which has not been available in the fluorescence microscopy technology until today. A doubling of the resolution of a conventional fluorescence microscopy has been achieved with the SR-SIM (Super-resolution Structured Illumination Microscopy) technology.
The necessary labeling can be performed with all common fluorescent dyes. The PALM (Photoactivated Localization Microscopy) technology offers a localization accuracy of up to 10 nm using e.g. shiftable fluorescent proteins. The ELYRA PS.1 at the RTC Rostock combines the super-resolution technology with the LSM 780, which is the latest and most effective confocal Laser Scanning Microscope from Carl Zeiss.
Ingrid Rieck | idw
Nanoparticle Exposure Can Awaken Dormant Viruses in the Lungs
16.01.2017 | Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt
Cholera bacteria infect more effectively with a simple twist of shape
13.01.2017 | Princeton University
Researchers from the University of Hamburg in Germany, in collaboration with colleagues from the University of Aarhus in Denmark, have synthesized a new superconducting material by growing a few layers of an antiferromagnetic transition-metal chalcogenide on a bismuth-based topological insulator, both being non-superconducting materials.
While superconductivity and magnetism are generally believed to be mutually exclusive, surprisingly, in this new material, superconducting correlations...
Laser-driving of semimetals allows creating novel quasiparticle states within condensed matter systems and switching between different states on ultrafast time scales
Studying properties of fundamental particles in condensed matter systems is a promising approach to quantum field theory. Quasiparticles offer the opportunity...
Among the general public, solar thermal energy is currently associated with dark blue, rectangular collectors on building roofs. Technologies are needed for aesthetically high quality architecture which offer the architect more room for manoeuvre when it comes to low- and plus-energy buildings. With the “ArKol” project, researchers at Fraunhofer ISE together with partners are currently developing two façade collectors for solar thermal energy generation, which permit a high degree of design flexibility: a strip collector for opaque façade sections and a solar thermal blind for transparent sections. The current state of the two developments will be presented at the BAU 2017 trade fair.
As part of the “ArKol – development of architecturally highly integrated façade collectors with heat pipes” project, Fraunhofer ISE together with its partners...
At TU Wien, an alternative for resource intensive formwork for the construction of concrete domes was developed. It is now used in a test dome for the Austrian Federal Railways Infrastructure (ÖBB Infrastruktur).
Concrete shells are efficient structures, but not very resource efficient. The formwork for the construction of concrete domes alone requires a high amount of...
Many pathogens use certain sugar compounds from their host to help conceal themselves against the immune system. Scientists at the University of Bonn have now, in cooperation with researchers at the University of York in the United Kingdom, analyzed the dynamics of a bacterial molecule that is involved in this process. They demonstrate that the protein grabs onto the sugar molecule with a Pac Man-like chewing motion and holds it until it can be used. Their results could help design therapeutics that could make the protein poorer at grabbing and holding and hence compromise the pathogen in the host. The study has now been published in “Biophysical Journal”.
The cells of the mouth, nose and intestinal mucosa produce large quantities of a chemical called sialic acid. Many bacteria possess a special transport system...
10.01.2017 | Event News
09.01.2017 | Event News
05.01.2017 | Event News
17.01.2017 | Earth Sciences
17.01.2017 | Materials Sciences
17.01.2017 | Architecture and Construction