Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

The Key that Fits

05.03.2010
New Technique To Trace Disease-Related Agents

In the development of new drugs, photoaffinity labels (PALs) are a versatile tool to investigate the interaction between a receptor and a drug or a ligand. Researchers working with Stephanie Grond at the University of Tübingen and Paultheo von Zezschwitz at the University of Marburg have now developed a new class of PALs that can be attached to the ligand in a single step, thus causing less modification of its structure, as they report in the European Journal of Organic Chemistry. The group has tested the new labels on an enzyme that is linked to osteoporosis and cancer.

When PALs are used to study a specific ligand, the ligand is firstly decorated with a chemical group that can be activated when exposed to ultraviolet light, whereupon it irreversibly binds to the receptor to form a stable complex. This complex can then be thoroughly studied, e.g., by fragmentation of the receptor. Typically, formation and fragmentation of the ligand–receptor complex is traced by monitoring special tags that are incorporated into the complex. However, these tags are problematic due to high costs and size restrictions, and frequently, the tagged fragments cannot be detected among the vast excess of untagged fragments. Therefore, any potential to study the complexes is lost. Finding new ways to separate the tagged fragments from the untagged ones is one of the major challenges facing scientists today.

Grond and von Zezschwitz, together with the help of their biology colleagues Markus Huss and Helmut Wieczorek at the University of Osnabrück, have been studying V-ATPase, which is an enzyme that has been linked to osteoporosis and some cancers, to determine its mode of inhibition. To do so, they have developed a new fluorous photoaffinity label (F-PAL) that can be attached to the ligand in one step, as both the activator and the tag are contained within the same compound. The F-PAL contains a long carbon chain that is fully substituted with fluorine atoms instead of the usual hydrogen atoms. By using a special separation technique that is specific to fluorine-containing compounds, compounds with a high fluorine content can be easily “fished out” from untagged compounds. Once the tagged fragments of the ligand–receptor complexes are isolated, they can be analyzed to unravel the exact function of the drug, which could give scientists some valuable insight into how diseases such as osteoporosis and cancer can be fought.

Author: Stephanie Grond, Eberhard-Karls-Universität Tübingen (Germany), http://www.grond.chemie.uni-goettingen.de/

Title: New Fluorous Photoaffinity Labels (F-PAL) and Their Application in V-ATPase Inhibition Studies

European Journal of Organic Chemistry, Permalink: http://dx.doi.org/10.1002/ejoc.200901463

Stephanie Grond | European Journal of Organic Chem
Further information:
http://www.grond.chemie.uni-goettingen.de/
http://www.eurjoc.org

More articles from Life Sciences:

nachricht Transport of molecular motors into cilia
28.03.2017 | Aarhus University

nachricht Asian dust providing key nutrients for California's giant sequoias
28.03.2017 | University of California - Riverside

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: A Challenging European Research Project to Develop New Tiny Microscopes

The Institute of Semiconductor Technology and the Institute of Physical and Theoretical Chemistry, both members of the Laboratory for Emerging Nanometrology (LENA), at Technische Universität Braunschweig are partners in a new European research project entitled ChipScope, which aims to develop a completely new and extremely small optical microscope capable of observing the interior of living cells in real time. A consortium of 7 partners from 5 countries will tackle this issue with very ambitious objectives during a four-year research program.

To demonstrate the usefulness of this new scientific tool, at the end of the project the developed chip-sized microscope will be used to observe in real-time...

Im Focus: Giant Magnetic Fields in the Universe

Astronomers from Bonn and Tautenburg in Thuringia (Germany) used the 100-m radio telescope at Effelsberg to observe several galaxy clusters. At the edges of these large accumulations of dark matter, stellar systems (galaxies), hot gas, and charged particles, they found magnetic fields that are exceptionally ordered over distances of many million light years. This makes them the most extended magnetic fields in the universe known so far.

The results will be published on March 22 in the journal „Astronomy & Astrophysics“.

Galaxy clusters are the largest gravitationally bound structures in the universe. With a typical extent of about 10 million light years, i.e. 100 times the...

Im Focus: Tracing down linear ubiquitination

Researchers at the Goethe University Frankfurt, together with partners from the University of Tübingen in Germany and Queen Mary University as well as Francis Crick Institute from London (UK) have developed a novel technology to decipher the secret ubiquitin code.

Ubiquitin is a small protein that can be linked to other cellular proteins, thereby controlling and modulating their functions. The attachment occurs in many...

Im Focus: Perovskite edges can be tuned for optoelectronic performance

Layered 2D material improves efficiency for solar cells and LEDs

In the eternal search for next generation high-efficiency solar cells and LEDs, scientists at Los Alamos National Laboratory and their partners are creating...

Im Focus: Polymer-coated silicon nanosheets as alternative to graphene: A perfect team for nanoelectronics

Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are less stable. Now researchers at the Technical University of Munich (TUM) have, for the first time ever, produced a composite material combining silicon nanosheets and a polymer that is both UV-resistant and easy to process. This brings the scientists a significant step closer to industrial applications like flexible displays and photosensors.

Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

International Land Use Symposium ILUS 2017: Call for Abstracts and Registration open

20.03.2017 | Event News

CONNECT 2017: International congress on connective tissue

14.03.2017 | Event News

ICTM Conference: Turbine Construction between Big Data and Additive Manufacturing

07.03.2017 | Event News

 
Latest News

Transport of molecular motors into cilia

28.03.2017 | Life Sciences

A novel hybrid UAV that may change the way people operate drones

28.03.2017 | Information Technology

NASA spacecraft investigate clues in radiation belts

28.03.2017 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>