Enzymes can be efficient biocatalysts for organic synthesis, but often only work on a small range of specific substrates.
Yoshihito Watanabe and colleagues at Nagoya University and RIKEN (Japan) have tricked enzymes into catalyzing reactions between molecules of choice, they report in Chemistry—An Asian Journal. By using organic acids, including the pain reliever ibuprofen, they mimicked the natural substrate.
The researchers used one member of the family of so-called cytochrome P450 enzymes, which owe their catalytic activity to the same heme group that binds oxygen in hemoglobin in the blood. They were able to expand its substrate scope by using organic acids to simulate substrate binding. The carboxylic acid group activates the H2O2 oxidant, which finally results in oxidation of the heme iron atom and generation of the active species.
This "decoy" approach allows epoxidation of styrene, which cannot normally be achieved with P450 enzymes. When an asymmetric decoy is used, such as the common pain reliever ibuprofen, the epoxidation proceeds stereoselectively: Only the desired molecule is produced, but not its mirror image, which can make the difference between a drug and a poison.
Further optimization of the system both in terms of decoy molecule and P450 mutant should yield higher catalytic activity and better stereoselectivity. This method should prove valuable in expanding the use of enzymes in organic synthesis.About the Author
Chemistry - An Asian Journal, Permalink to the article: http://dx.doi.org/10.1002/asia.201200250
The birth of a new protein
20.10.2017 | University of Arizona
Building New Moss Factories
20.10.2017 | Albert-Ludwigs-Universität Freiburg im Breisgau
University of Maryland researchers contribute to historic detection of gravitational waves and light created by event
On August 17, 2017, at 12:41:04 UTC, scientists made the first direct observation of a merger between two neutron stars--the dense, collapsed cores that remain...
Seven new papers describe the first-ever detection of light from a gravitational wave source. The event, caused by two neutron stars colliding and merging together, was dubbed GW170817 because it sent ripples through space-time that reached Earth on 2017 August 17. Around the world, hundreds of excited astronomers mobilized quickly and were able to observe the event using numerous telescopes, providing a wealth of new data.
Previous detections of gravitational waves have all involved the merger of two black holes, a feat that won the 2017 Nobel Prize in Physics earlier this month....
Material defects in end products can quickly result in failures in many areas of industry, and have a massive impact on the safe use of their products. This is why, in the field of quality assurance, intelligent, nondestructive sensor systems play a key role. They allow testing components and parts in a rapid and cost-efficient manner without destroying the actual product or changing its surface. Experts from the Fraunhofer IZFP in Saarbrücken will be presenting two exhibits at the Blechexpo in Stuttgart from 7–10 November 2017 that allow fast, reliable, and automated characterization of materials and detection of defects (Hall 5, Booth 5306).
When quality testing uses time-consuming destructive test methods, it can result in enormous costs due to damaging or destroying the products. And given that...
Using a new cooling technique MPQ scientists succeed at observing collisions in a dense beam of cold and slow dipolar molecules.
How do chemical reactions proceed at extremely low temperatures? The answer requires the investigation of molecular samples that are cold, dense, and slow at...
Scientists from the Max Planck Institute of Quantum Optics, using high precision laser spectroscopy of atomic hydrogen, confirm the surprisingly small value of the proton radius determined from muonic hydrogen.
It was one of the breakthroughs of the year 2010: Laser spectroscopy of muonic hydrogen resulted in a value for the proton charge radius that was significantly...
17.10.2017 | Event News
10.10.2017 | Event News
10.10.2017 | Event News
20.10.2017 | Information Technology
20.10.2017 | Materials Sciences
20.10.2017 | Interdisciplinary Research