One common way of studying the role of genes in cells is to remove a gene and investigate the effect of the loss. Genes are very similar in both yeast and people, which is one reason why the baker’s and brewer’s yeast Saccharomyces cerevisiae has become a firm favourite with geneticists – and in yeast it is easy to make this kind of genetic change.
However, this does not work for many genes as the loss causes the cells to die. These are known as essential genes and are therefore difficult to study. This is a major problem for researchers as essential genes are often involved in crucial life processes. These essential genes are also the most well-conserved over long evolutionary distances, like between humans and yeast.
Together with researchers from the University of Toronto, Anders Blomberg and Jonas Warringer from the University of Gothenburg’s Department of Cell- and Molecular Biology have produced a collection of nearly 800 strains of yeast cells where the function of these essential genes can be studied. This new genetic tool is now being made available to other researchers.
“The trick is to use temperature-sensitive mutants for the genes you want to study,” says professor Anders Blomberg. “These mutants have amino acid changes, which make the resultant protein sensitive to higher temperatures but able to function normally at normal temperatures. And at intermediary temperatures one can set the desired activity of the mutant protein.”
The Gothenburg researchers have worked for many years on characterising the changes in yeast mutants that result from genetic changes or environmental factors automatically and on a large scale. They will continue to develop and characterize the new collection of yeast cells to facilitate the systematic analysis of the function of all essential genes.
The applications of this genetic tool are exemplified in an article published in the scientific journal Nature Biotechnology.
Authors: Zhijian Li, Franco J Vizeacoumar, Sondra Bahr, Jingjing Li, Jonas Warringer, Frederick S Vizeacoumar, Renqiang Min, Benjamin VanderSluis, Jeremy Bellay, Michael DeVit, James A Fleming, Andrew Stephens, Julian Haase, Zhen-Yuan Lin, Anastasia Baryshnikova, Hong Lu, Zhun Yan, Ke Jin, Sarah Barker, Alessandro Datti, Guri Giaever, Corey Nislow, Chris Bulawa, Chad L Myers, Michael Costanzo, Anne-Claude Gingras, Zhaolei ZhangFor more information, please contact:
email@example.comJonas Warringer, Department of Cell- and Molecular Biology, University of Gothenburg, tel: +46 (0)31 786 3961
Helena Aaberg | idw
Enduring cold temperatures alters fat cell epigenetics
19.04.2018 | University of Tokyo
Full of hot air and proud of it
18.04.2018 | University of Pittsburgh
Study published in the journal ACS Applied Materials & Interfaces is the outcome of an international effort that included teams from Dresden and Berlin in Germany, and the US.
Scientists at the Helmholtz-Zentrum Dresden-Rossendorf (HZDR) together with colleagues from the Helmholtz-Zentrum Berlin (HZB) and the University of Virginia...
Novel highly efficient and brilliant gamma-ray source: Based on model calculations, physicists of the Max PIanck Institute for Nuclear Physics in Heidelberg propose a novel method for an efficient high-brilliance gamma-ray source. A giant collimated gamma-ray pulse is generated from the interaction of a dense ultra-relativistic electron beam with a thin solid conductor. Energetic gamma-rays are copiously produced as the electron beam splits into filaments while propagating across the conductor. The resulting gamma-ray energy and flux enable novel experiments in nuclear and fundamental physics.
The typical wavelength of light interacting with an object of the microcosm scales with the size of this object. For atoms, this ranges from visible light to...
Stable joint cartilage can be produced from adult stem cells originating from bone marrow. This is made possible by inducing specific molecular processes occurring during embryonic cartilage formation, as researchers from the University and University Hospital of Basel report in the scientific journal PNAS.
Certain mesenchymal stem/stromal cells from the bone marrow of adults are considered extremely promising for skeletal tissue regeneration. These adult stem...
In the fight against cancer, scientists are developing new drugs to hit tumor cells at so far unused weak points. Such a “sore spot” is the protein complex...
In an article that appears in the journal “Review of Modern Physics”, researchers at the Laboratory for Attosecond Physics (LAP) assess the current state of the field of ultrafast physics and consider its implications for future technologies.
Physicists can now control light in both time and space with hitherto unimagined precision. This is particularly true for the ability to generate ultrashort...
13.04.2018 | Event News
12.04.2018 | Event News
09.04.2018 | Event News
19.04.2018 | Materials Sciences
19.04.2018 | Physics and Astronomy
19.04.2018 | Physics and Astronomy