One common way of studying the role of genes in cells is to remove a gene and investigate the effect of the loss. Genes are very similar in both yeast and people, which is one reason why the baker’s and brewer’s yeast Saccharomyces cerevisiae has become a firm favourite with geneticists – and in yeast it is easy to make this kind of genetic change.
However, this does not work for many genes as the loss causes the cells to die. These are known as essential genes and are therefore difficult to study. This is a major problem for researchers as essential genes are often involved in crucial life processes. These essential genes are also the most well-conserved over long evolutionary distances, like between humans and yeast.
Together with researchers from the University of Toronto, Anders Blomberg and Jonas Warringer from the University of Gothenburg’s Department of Cell- and Molecular Biology have produced a collection of nearly 800 strains of yeast cells where the function of these essential genes can be studied. This new genetic tool is now being made available to other researchers.
“The trick is to use temperature-sensitive mutants for the genes you want to study,” says professor Anders Blomberg. “These mutants have amino acid changes, which make the resultant protein sensitive to higher temperatures but able to function normally at normal temperatures. And at intermediary temperatures one can set the desired activity of the mutant protein.”
The Gothenburg researchers have worked for many years on characterising the changes in yeast mutants that result from genetic changes or environmental factors automatically and on a large scale. They will continue to develop and characterize the new collection of yeast cells to facilitate the systematic analysis of the function of all essential genes.
The applications of this genetic tool are exemplified in an article published in the scientific journal Nature Biotechnology.
Authors: Zhijian Li, Franco J Vizeacoumar, Sondra Bahr, Jingjing Li, Jonas Warringer, Frederick S Vizeacoumar, Renqiang Min, Benjamin VanderSluis, Jeremy Bellay, Michael DeVit, James A Fleming, Andrew Stephens, Julian Haase, Zhen-Yuan Lin, Anastasia Baryshnikova, Hong Lu, Zhun Yan, Ke Jin, Sarah Barker, Alessandro Datti, Guri Giaever, Corey Nislow, Chris Bulawa, Chad L Myers, Michael Costanzo, Anne-Claude Gingras, Zhaolei ZhangFor more information, please contact:
firstname.lastname@example.orgJonas Warringer, Department of Cell- and Molecular Biology, University of Gothenburg, tel: +46 (0)31 786 3961
Helena Aaberg | idw
Fine organic particles in the atmosphere are more often solid glass beads than liquid oil droplets
21.04.2017 | Max-Planck-Institut für Chemie
Study overturns seminal research about the developing nervous system
21.04.2017 | University of California - Los Angeles Health Sciences
The nearby, giant radio galaxy M87 hosts a supermassive black hole (BH) and is well-known for its bright jet dominating the spectrum over ten orders of magnitude in frequency. Due to its proximity, jet prominence, and the large black hole mass, M87 is the best laboratory for investigating the formation, acceleration, and collimation of relativistic jets. A research team led by Silke Britzen from the Max Planck Institute for Radio Astronomy in Bonn, Germany, has found strong indication for turbulent processes connecting the accretion disk and the jet of that galaxy providing insights into the longstanding problem of the origin of astrophysical jets.
Supermassive black holes form some of the most enigmatic phenomena in astrophysics. Their enormous energy output is supposed to be generated by the...
The probability to find a certain number of photons inside a laser pulse usually corresponds to a classical distribution of independent events, the so-called...
Microprocessors based on atomically thin materials hold the promise of the evolution of traditional processors as well as new applications in the field of flexible electronics. Now, a TU Wien research team led by Thomas Müller has made a breakthrough in this field as part of an ongoing research project.
Two-dimensional materials, or 2D materials for short, are extremely versatile, although – or often more precisely because – they are made up of just one or a...
Two researchers at Heidelberg University have developed a model system that enables a better understanding of the processes in a quantum-physical experiment...
Glaciers might seem rather inhospitable environments. However, they are home to a diverse and vibrant microbial community. It’s becoming increasingly clear that they play a bigger role in the carbon cycle than previously thought.
A new study, now published in the journal Nature Geoscience, shows how microbial communities in melting glaciers contribute to the Earth’s carbon cycle, a...
20.04.2017 | Event News
18.04.2017 | Event News
03.04.2017 | Event News
21.04.2017 | Physics and Astronomy
21.04.2017 | Health and Medicine
21.04.2017 | Physics and Astronomy