Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Biomedical breakthrough: Blood vessels for lab-grown tissues

12.01.2011
Rice, BCM discovery addresses key roadblock to growing replacement tissues, organs

Researchers from Rice University and Baylor College of Medicine (BCM) have broken one of the major roadblocks on the path to growing transplantable tissue in the lab: They've found a way to grow the blood vessels and capillaries needed to keep tissues alive.

The new research is available online and due to appear in the January issue of the journal Acta Biomaterialia.

"The inability to grow blood-vessel networks -- or vasculature -- in lab-grown tissues is the leading problem in regenerative medicine today," said lead co-author Jennifer West, department chair and the Isabel C. Cameron Professor of Bioengineering at Rice. "If you don't have blood supply, you cannot make a tissue structure that is thicker than a couple hundred microns."

As its base material, a team of researchers led by West and BCM molecular physiologist Mary Dickinson chose polyethylene glycol (PEG), a nontoxic plastic that's widely used in medical devices and food. Building on 10 years of research in West's lab, the scientists modified the PEG to mimic the body's extracellular matrix -- the network of proteins and polysaccharides that make up a substantial portion of most tissues.

West, Dickinson, Rice graduate student Jennifer Saik, Rice undergraduate Emily Watkins and Rice-BCM graduate student Daniel Gould combined the modified PEG with two kinds of cells -- both of which are needed for blood-vessel formation. Using light that locks the PEG polymer strands into a solid gel, they created soft hydrogels that contained living cells and growth factors. After that, they filmed the hydrogels for 72 hours. By tagging each type of cell with a different colored fluorescent marker, the team was able to watch as the cells gradually formed capillaries throughout the soft, plastic gel.

To test these new vascular networks, the team implanted the hydrogels into the corneas of mice, where no natural vasculature exists. After injecting a dye into the mice's bloodstream, the researchers confirmed normal blood flow in the newly grown capillaries.

Another key advance, published by West and graduate student Joseph Hoffmann in November, involved the creation of a new technique called "two-photon lithography," an ultrasensitive way of using light to create intricate three-dimensional patterns within the soft PEG hydrogels. West said the patterning technique allows the engineers to exert a fine level of control over where cells move and grow. In follow-up experiments, also in collaboration with the Dickinson lab at BCM, West and her team plan to use the technique to grow blood vessels in predetermined patterns.

The research was supported by the National Science Foundation and the National Institutes of Health. West's work was conducted in her lab at Rice's BioScience Research Collaborative (BRC). The BRC is an innovative space where scientists and educators from Rice University and other Texas Medical Center institutions work together to perform leading research that benefits human medicine and health.

Related materials:

To read the complete study, go to http://tinyurl.com/5s676qz.

A video is available here at http://www.youtube.com/watch?v=JtMifCkTHTo.

Caption: This time-lapse image shows how two types of cells -- which were tagged with fluorescent dye -- organize themselves into a functioning capillary networks within 72 hours.

A photo of Jennifer West is available at http://www.rice.edu/nationalmedia/images/jennifer-west.

Credit: Jeff Fitlow/Rice University

Caption: Rice University bioengineering professor Jennifer West (right) and graduate student Jennifer Saik.

Located in Houston, Rice University is consistently ranked one of America's best teaching and research universities. Known for its "unconventional wisdom," Rice is distinguished by its: size -- 3,485 undergraduates and 2,275 graduate students; selectivity -- 13 applicants for each place in the freshman class; resources -- an undergraduate student-to-faculty ratio of less than 6-to-1; sixth largest endowment per student among American private research universities; residential college system, which builds communities that are both close-knit and diverse; and collaborative culture, which crosses disciplines, integrates teaching and research, and intermingles undergraduate and graduate work.

David Ruth | EurekAlert!
Further information:
http://www.rice.edu

More articles from Life Sciences:

nachricht Nesting aids make agricultural fields attractive for bees
20.07.2017 | Julius-Maximilians-Universität Würzburg

nachricht The Kitchen Sponge – Breeding Ground for Germs
20.07.2017 | Hochschule Furtwangen

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Manipulating Electron Spins Without Loss of Information

Physicists have developed a new technique that uses electrical voltages to control the electron spin on a chip. The newly-developed method provides protection from spin decay, meaning that the contained information can be maintained and transmitted over comparatively large distances, as has been demonstrated by a team from the University of Basel’s Department of Physics and the Swiss Nanoscience Institute. The results have been published in Physical Review X.

For several years, researchers have been trying to use the spin of an electron to store and transmit information. The spin of each electron is always coupled...

Im Focus: The proton precisely weighted

What is the mass of a proton? Scientists from Germany and Japan successfully did an important step towards the most exact knowledge of this fundamental constant. By means of precision measurements on a single proton, they could improve the precision by a factor of three and also correct the existing value.

To determine the mass of a single proton still more accurate – a group of physicists led by Klaus Blaum and Sven Sturm of the Max Planck Institute for Nuclear...

Im Focus: On the way to a biological alternative

A bacterial enzyme enables reactions that open up alternatives to key industrial chemical processes

The research team of Prof. Dr. Oliver Einsle at the University of Freiburg's Institute of Biochemistry has long been exploring the functioning of nitrogenase....

Im Focus: The 1 trillion tonne iceberg

Larsen C Ice Shelf rift finally breaks through

A one trillion tonne iceberg - one of the biggest ever recorded -- has calved away from the Larsen C Ice Shelf in Antarctica, after a rift in the ice,...

Im Focus: Laser-cooled ions contribute to better understanding of friction

Physics supports biology: Researchers from PTB have developed a model system to investigate friction phenomena with atomic precision

Friction: what you want from car brakes, otherwise rather a nuisance. In any case, it is useful to know as precisely as possible how friction phenomena arise –...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

»We are bringing Additive Manufacturing to SMEs«

19.07.2017 | Event News

The technology with a feel for feelings

12.07.2017 | Event News

Leipzig HTP-Forum discusses "hydrothermal processes" as a key technology for a biobased economy

12.07.2017 | Event News

 
Latest News

Researchers create new technique for manipulating polarization of terahertz radiation

20.07.2017 | Information Technology

High-tech sensing illuminates concrete stress testing

20.07.2017 | Materials Sciences

First direct observation and measurement of ultra-fast moving vortices in superconductors

20.07.2017 | Physics and Astronomy

VideoLinks
B2B-VideoLinks
More VideoLinks >>>