Fish-like animal emanates bright and dim versions of fluorescent light, a phenomenon that could help guide human biotechnological applications
Pushing closer to understanding the mechanisms behind the mysterious glow of light produced naturally by certain animals, scientists at Scripps Institution of Oceanography at UC San Diego have deciphered the structural components related to fluorescence brightness in a primitive sea creature.
In a study published in Scientific Reports, an open-access journal of the Nature Publishing Group, Dimitri Deheyn and his colleagues at Scripps Oceanography, the Air Force Research Laboratory, and the Salk Institute for Biological Studies have conducted the most detailed examination of green fluorescent proteins (GFPs) in lancelets, marine invertebrates also known as “amphioxus.”
The fish-shaped animals, which spend much of their time in shallow coastal regions burrowed in sand except for their heads, offer unique insights on natural fluorescence since individual specimens can emit both very bright and much dimmer versions of the light, a rare capability in the animal kingdom.
The study carries implications for a variety of industries looking to maximize brightness of natural fluorescence—the process of transformation of blue “excitation” light into green “emission” light—including applications in biotechnology such as adapting fluorescence for biomedical protein tracers and for tracking the expression of specific genes in the human body.
In investigating the structural differences between the proteins with the two levels of light output, known to be generated by the GFPs inside amphioxus, Deheyn and his colleagues found that only a few key structural differences at the nanoscale allows the sea creature to emit different brightness levels. The differences relate to changes in stiffness around the animal’s “chromophore pocket,” the area of proteins responsible for molecular transformation of light, and thus light output intensity.
“We discovered that some of the amphioxus GFPs are able to transform blue light into green light with 100 percent efficiency (current engineered GFPs—traditionally rooted in the Cnidarian phylum—only reach 60 to 80 percent efficiency), which combines with other properties of light absorbance to make the amphioxus GFPs about five times brighter than current commercially available GFPs, resulting in effect to a huge difference,” said Deheyn. “It is also interesting that the same animal will also express similar GFPs with an efficiency of about 1,000 times less.”
The exact mechanism that controls this ability of perfect efficiency during light transformation from blue to green remains unknown, Deheyn said, but this study opens doors towards its understanding.
“The most unique part of this discovery perhaps lays in the fact that for the first time, we show that different GFPs seem to have different functions within the same individual and unrelated to their ability to produce light, thus probably involving a biochemical role as well,” said Deheyn. “Nevertheless, having bright GFPs or the tool to increase brightness in current ones is critical for optimizing applications of fluorescence.”
Amphioxus are thought to use fluorescence for photo-protection (thus acting as sunscreen), as an antioxidant, and possibly for photo-sensing (using GFPs as receptors to the surrounding light) in their environment. Deheyn says learning more about bright-emitting GFPs in nature is useful for a variety of applications and fields of science.
“The U.S. Air Force, and the Department of Defense in general, uses a large variety of biosensors in biomedicine, bioengineering, and materials science, and providing proteins with the ability to be very bright can help technology advance because of better signal-to-noise ratio.”
Coauthors of the paper include Erin Bomati of Scripps Oceanography; Joy Haley of the Air Force Research Laboratory; and Joseph Noel of the Salk Institute for Biological Studies. The Air Force Office of Scientific Research supported the study.
Mario Aguilera | Eurek Alert!
A Map of the Cell’s Power Station
18.08.2017 | Albert-Ludwigs-Universität Freiburg im Breisgau
On the way to developing a new active ingredient against chronic infections
18.08.2017 | Deutsches Zentrum für Infektionsforschung
Whether you call it effervescent, fizzy, or sparkling, carbonated water is making a comeback as a beverage. Aside from quenching thirst, researchers at the University of Illinois at Urbana-Champaign have discovered a new use for these "bubbly" concoctions that will have major impact on the manufacturer of the world's thinnest, flattest, and one most useful materials -- graphene.
As graphene's popularity grows as an advanced "wonder" material, the speed and quality at which it can be manufactured will be paramount. With that in mind,...
Physicists at the University of Bonn have managed to create optical hollows and more complex patterns into which the light of a Bose-Einstein condensate flows. The creation of such highly low-loss structures for light is a prerequisite for complex light circuits, such as for quantum information processing for a new generation of computers. The researchers are now presenting their results in the journal Nature Photonics.
Light particles (photons) occur as tiny, indivisible portions. Many thousands of these light portions can be merged to form a single super-photon if they are...
For the first time, scientists have shown that circular RNA is linked to brain function. When a RNA molecule called Cdr1as was deleted from the genome of mice, the animals had problems filtering out unnecessary information – like patients suffering from neuropsychiatric disorders.
While hundreds of circular RNAs (circRNAs) are abundant in mammalian brains, one big question has remained unanswered: What are they actually good for? In the...
An experimental small satellite has successfully collected and delivered data on a key measurement for predicting changes in Earth's climate.
The Radiometer Assessment using Vertically Aligned Nanotubes (RAVAN) CubeSat was launched into low-Earth orbit on Nov. 11, 2016, in order to test new...
A study led by scientists of the Max Planck Institute for the Structure and Dynamics of Matter (MPSD) at the Center for Free-Electron Laser Science in Hamburg presents evidence of the coexistence of superconductivity and “charge-density-waves” in compounds of the poorly-studied family of bismuthates. This observation opens up new perspectives for a deeper understanding of the phenomenon of high-temperature superconductivity, a topic which is at the core of condensed matter research since more than 30 years. The paper by Nicoletti et al has been published in the PNAS.
Since the beginning of the 20th century, superconductivity had been observed in some metals at temperatures only a few degrees above the absolute zero (minus...
16.08.2017 | Event News
04.08.2017 | Event News
26.07.2017 | Event News
18.08.2017 | Life Sciences
18.08.2017 | Physics and Astronomy
18.08.2017 | Materials Sciences