After the mare died at the Cornell Hospital for Animals, veterinarian Sylvia Bedford-Guaus and her laboratory team " graduate student Lori McPartlin and technician Stephanie Twomey " made use of special tools and their expertise from years of research to scrape the wall of each follicle in both of the ovaries obtained from the mare, Rebaqua.
"The process is time-sensitive and very intricate," said Bedford-Guaus, explaining that the eggs (oocytes) can only be seen microscopically and must be collected from the ovary within a few hours.
"There were other approaches, such as shipping the entire ovary, but none that could offer the success rates we had with this procedure," she said, adding that when equine ovaries have been shipped before, few pregnancies have resulted in no live births because the oocytes do not stay viable within the ovaries.
Bedford-Guaus searched for oocytes under a dissecting microscope and washed them in a special medium. The process took more than three hours. "Racing against the clock," said Bedford-Guaus, the team packaged nine oocytes for overnight shipment to veterinarian Katrin Hinrichs Equine Embryo Laboratory at Texas A&M University, where the researchers had the expertise needed to fertilize the eggs.
In Texas, the oocytes were incubated in a medium that stimulated five of the oocytes to mature; they were then fertilized with frozen-thawed sperm from the third-ranked barrel-racing stallion in the nation, Frenchmans Guy. This procedure, performed by veterinarian Young-Ho Choi, used a micromanipulation technique termed intracytoplasmic sperm injection. The fertilized eggs were cultured for seven days. Two developed into embryos, which were then sent to veterinarian David Hartman at the Hartman Equine Reproduction Center in Whitesboro, Texas, who transferred them to a surrogate mare that was at the right stage of the cycle to carry a pregnancy. The mare was then purchased by Rebaqua's owner, Kristin Contro of Binghamton, who had the pregnant mare shipped to New York.
"Because there are so many horses in the world, prior to Rebaqua's never felt it was necessary to breed a horse," said Contro, who began riding when she was 10 and has owned more than 10 horses. "Rebaqua was as close to perfect as you could get. She was bred really well, so her genes were superb. Her confirmation was perfect. And, she was a champion barrel racer. I am hopeful that her foal will also have her heart. That's not something you can breed for. They either have it or they don't."
(Text by Stephanie Specchio of Cornell's College of Veterinary Medicine.)
Joe Schwartz | Newswise Science News
Energy crop production on conservation lands may not boost greenhouse gases
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How nature creates forest diversity
07.03.2017 | International Institute for Applied Systems Analysis (IIASA)
Astronomers from Bonn and Tautenburg in Thuringia (Germany) used the 100-m radio telescope at Effelsberg to observe several galaxy clusters. At the edges of these large accumulations of dark matter, stellar systems (galaxies), hot gas, and charged particles, they found magnetic fields that are exceptionally ordered over distances of many million light years. This makes them the most extended magnetic fields in the universe known so far.
The results will be published on March 22 in the journal „Astronomy & Astrophysics“.
Galaxy clusters are the largest gravitationally bound structures in the universe. With a typical extent of about 10 million light years, i.e. 100 times the...
Researchers at the Goethe University Frankfurt, together with partners from the University of Tübingen in Germany and Queen Mary University as well as Francis Crick Institute from London (UK) have developed a novel technology to decipher the secret ubiquitin code.
Ubiquitin is a small protein that can be linked to other cellular proteins, thereby controlling and modulating their functions. The attachment occurs in many...
In the eternal search for next generation high-efficiency solar cells and LEDs, scientists at Los Alamos National Laboratory and their partners are creating...
Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are less stable. Now researchers at the Technical University of Munich (TUM) have, for the first time ever, produced a composite material combining silicon nanosheets and a polymer that is both UV-resistant and easy to process. This brings the scientists a significant step closer to industrial applications like flexible displays and photosensors.
Silicon nanosheets are thin, two-dimensional layers with exceptional optoelectronic properties very similar to those of graphene. Albeit, the nanosheets are...
Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to simulate these confined natural conditions in artificial vesicles for the first time. As reported in the academic journal Small, the results are offering better insight into the development of nanoreactors and artificial organelles.
Enzymes behave differently in a test tube compared with the molecular scrum of a living cell. Chemists from the University of Basel have now been able to...
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