Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Vanderbilt Team First to Blend High-End Imaging Techniques

17.03.2015

Vanderbilt University researchers have achieved the first “image fusion” of mass spectrometry and microscopy — a technical tour de force that could, among other things, dramatically improve the diagnosis and treatment of cancer.

Microscopy can yield high-resolution images of tissues, but “it really doesn’t give you molecular information,” said Richard Caprioli, Ph.D., senior author of the paper published last week in the journal Nature Methods.


Courtesy of Vanderbilt University Medical Center

Image of a section of the brain shows the fusion of microscopy (pink area) and mass spectrometry (pixelated colors at bottom) to produce a detailed “map” of the distribution of proteins, lipids and other molecules within sharply delineated brain structures (upper left).

Mass spectrometry provides a very precise accounting of the proteins, lipids and other molecules in a given tissue, but in a spatially coarse or pixelated manner.

Combining the best features of both imaging modalities allows scientists to see the molecular make-up of tissues in high resolution.

“That to me is just phenomenal,” said Caprioli, the Stanford Moore Professor of Biochemistry and director of the Mass Spectrometry Research Center.

Caprioli said the technique could redefine the surgical “margin,” the line between cancer cells and normal cells where the scalpel goes to remove the tumor.

Currently that line is determined by histology — the appearance of cells examined under the microscope. But many cancers recur after surgery. That could be because what appear to be normal cells, when analyzed for their protein content using mass spectrometry, are actually cancer cells in the making.

“The application of image fusion approaches to the analysis of tissue sections by microscopy and mass spectrometry is a significant innovation that should change the way that these techniques are used together,” said Douglas Sheeley, Sc.D., senior scientific officer in the National Institute of General Medical Sciences (NIGMS).

“It is an important step in the process of making mass spectrometry data accessible and truly useful for clinicians,” he said. The NIGMS, part of the National Institutes of Health (NIH), partially funded the research (grant numbers GM058008 and GM103391).

The image fusion project was led by Raf Van de Plas, Ph.D., a research assistant professor of Biochemistry who also has a faculty position at Delft University of Technology in the Netherlands. Other co-authors were postdoctoral fellow Junhai Yang, Ph.D., and Jeffrey Spraggins, Ph.D., research assistant professor of Biochemistry.

Using a mathematical approach called regression analysis, the researchers mapped each pixel of mass spectrometry data onto the corresponding spot on the microscopy image to produce a new, “predicted” image.

It’s similar in concept to the line drawn between experimentally determined points in a standard curve, Caprioli said. There are no “real” points between those that were actually measured, yet the line is predicted by the previous experiments.

In the same way, “we’re predicting what the data should look like,” he said.

Last year Caprioli was honored by the American Society for Mass Spectrometry for developing Imaging Mass Spectrometry (IMS) using matrix-assisted laser desorption/ionization (MALDI), a technique for visualizing proteins, lipids and other molecules in cells and tissues.

The introduction of this technology, essentially a “molecular microscope,” helps reveal the function of these molecules and how function is changed by diseases like cancer.

Contact Information
Craig Boerner
Media Director
craig.boerner@Vanderbilt.Edu
Phone: 615-322-4747
Mobile: 615-479-6374

Craig Boerner | newswise
Further information:
http://www.vanderbilt.edu

More articles from Medical Engineering:

nachricht PET identifies which prostate cancer patients can benefit from salvage radiation treatment
05.12.2017 | Society of Nuclear Medicine and Molecular Imaging

nachricht Designing a golden nanopill
01.12.2017 | University of Texas at Austin, Texas Advanced Computing Center

All articles from Medical Engineering >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: First-of-its-kind chemical oscillator offers new level of molecular control

DNA molecules that follow specific instructions could offer more precise molecular control of synthetic chemical systems, a discovery that opens the door for engineers to create molecular machines with new and complex behaviors.

Researchers have created chemical amplifiers and a chemical oscillator using a systematic method that has the potential to embed sophisticated circuit...

Im Focus: Long-lived storage of a photonic qubit for worldwide teleportation

MPQ scientists achieve long storage times for photonic quantum bits which break the lower bound for direct teleportation in a global quantum network.

Concerning the development of quantum memories for the realization of global quantum networks, scientists of the Quantum Dynamics Division led by Professor...

Im Focus: Electromagnetic water cloak eliminates drag and wake

Detailed calculations show water cloaks are feasible with today's technology

Researchers have developed a water cloaking concept based on electromagnetic forces that could eliminate an object's wake, greatly reducing its drag while...

Im Focus: Scientists channel graphene to understand filtration and ion transport into cells

Tiny pores at a cell's entryway act as miniature bouncers, letting in some electrically charged atoms--ions--but blocking others. Operating as exquisitely sensitive filters, these "ion channels" play a critical role in biological functions such as muscle contraction and the firing of brain cells.

To rapidly transport the right ions through the cell membrane, the tiny channels rely on a complex interplay between the ions and surrounding molecules,...

Im Focus: Towards data storage at the single molecule level

The miniaturization of the current technology of storage media is hindered by fundamental limits of quantum mechanics. A new approach consists in using so-called spin-crossover molecules as the smallest possible storage unit. Similar to normal hard drives, these special molecules can save information via their magnetic state. A research team from Kiel University has now managed to successfully place a new class of spin-crossover molecules onto a surface and to improve the molecule’s storage capacity. The storage density of conventional hard drives could therefore theoretically be increased by more than one hundred fold. The study has been published in the scientific journal Nano Letters.

Over the past few years, the building blocks of storage media have gotten ever smaller. But further miniaturization of the current technology is hindered by...

All Focus news of the innovation-report >>>

Anzeige

Anzeige

Event News

See, understand and experience the work of the future

11.12.2017 | Event News

Innovative strategies to tackle parasitic worms

08.12.2017 | Event News

AKL’18: The opportunities and challenges of digitalization in the laser industry

07.12.2017 | Event News

 
Latest News

Engineers program tiny robots to move, think like insects

15.12.2017 | Power and Electrical Engineering

One in 5 materials chemistry papers may be wrong, study suggests

15.12.2017 | Materials Sciences

New antbird species discovered in Peru by LSU ornithologists

15.12.2017 | Life Sciences

VideoLinks
B2B-VideoLinks
More VideoLinks >>>