Over the past years in the field of forensic medicine creation of DNA profiles with biological traces has gained in importance enormously. Individual-related attribution of DNA results from so-called STR-loci (short tandem repeats), which contain specific genetic information on every individual. Multiplex-PCR-analyses are used for DNA-typing, though frequently only a fractional DNA-profile can be created, if the DNA is strongly degraded or if there is only low amount of DNA available.
The innovation comprises a method in which two PCR reactions (a sixplex and a fiveplex PCR) are combined into one large multiplex reaction using biotinylated and fluorescence-marked primers. Those primers enable the amplification and separation of overlapping PCR-fragments.
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IMG Innovations-Management GmbH
Phone: +49 (0)631/31668-50
Contact
Dr. Klaus Kobek
