Young stars ejecting plasma could give us clues into the Sun’s past Kyoto, Japan — Down here on Earth we don’t usually notice, but the Sun is frequently ejecting huge masses of plasma into space. These are called coronal mass ejections (CMEs). They often occur together with sudden brightenings called flares, and sometimes extend far enough to disturb Earth’s magnetosphere, generating space weather phenomena including auroras or geomagnetic storms, and even damaging power grids on occasion. Scientists believe that when…
Metal nanostructures can act as tiny antennae to control light since they can focus and guide light on the smallest of scales. The optical properties of these…
What if the same technology could peer down to the level of atoms? Doctors could make visual diagnoses of a person’s molecules – examining damage on a strand…
The ECRG4 and ITIH5 (EI) gene promoter methylation has been identified as a novel objective biomarker panel for the diagnosis of bladder cancer (BLA) at an…
Part of the inflammatory response in Alzheimer´s disease (AD) is the upregulation of the inducible nitric oxide synthase NOS2 resulting in increased NO…
articularly in oncology determination of gene mutation statuses serve as a biomarker to guide therapeutic decisions.
Scientists of the University of…
Dirofilariasis is caused by Dirofilaria immitis and D. repens, parasitic nematodes, which are transmitted by blood sucking mosquitoes. The heartworms harbour…
Human filariasis (elephantiasis and river blindness) is caused by the nematodes W. bancrofti and O. volvulus, transmitted via blood sucking insects. The worms…
Diabetes mellitus is the most expensive chronic disorder, causing costs of about 40 billion Euros in 2010. More than 3/4 of these costs are associated with the…
The wide use of recombinant polypeptides is limited by still insufficient means of production, namely, their expression in host cells. The most often used…
The wide use of recombinant polypeptides is limited by still insufficient means of production, namely, their expression in host cells. The most often used bacterial host cells generally have the considerable drawback that polypeptides of eukaryotic origin are not folded correctly and that undesirable posttranslational modifications may occur. One of the most severe drawbacks is that the target polypeptide is typically located in the cytoplasm of the host cells and must be isolated from said cytoplasm in a laborious, time-consuming and expensive downstream-processing.
In view of the above, an expression system is needed that is usable in an eukaryotic cell extensively autologous with respect to sugars, amino acids and that is extensively independent of growth factors. It has been shown that plant cells, in particular microalgae cells, can be used for the production of recombinant proteins and overcome most of the above problems.
Microalga cultures are comparably easy to handle and, in principle, scalable to large production scales. Furthermore, microalgae merely require a slightly salty aqueous environment, CO2 and light, can be supplemented by simple carbon sources, or a combination of these strategies to grow rapidly and produce the recombinant protein of interest. Therefore, the cultivation of microalgae is relatively inexpensive. These cultures can be extensively free of sugars and amino acids. The use of solar energy to produce biomass and recombinant protein production is an attractive aspect of these cells in light of the goal of sustainability.
Unfortunately, the efficient production of recombinant proteins in microalgae is hampered because of the tightly regulated gene expression, preference for certain nucleic acid codon usage, and bias to its own promoters.
The present invention allows to overcome these problems with highly efficient production and direct secretion of target polypeptides into the medium and in addition enables simultaneous monitoring of the polypeptide production without disturbing the expression system.
This system has in principle a large range of applications and may be used due to the safe organism for researchers, pharmaceutical targets and food and feed producers.
This part of the constellation of Sagittarius (The Archer) is one of the richest star fields in the whole sky — the Large Sagittarius Star Cloud. The huge…
The wide use of recombinant polypeptides is limited by still insufficient means of production, namely, their expression in host cells. The most often used…
Entrapping sensitive material, e.g. immobilization of biomolecules or immobilization of other light-, pH- and/or temperature sensitive material is a demanding…
Tilapia has become the second most important fish in aquaculture after carp and before salmon. Consumers are starting to demand hormone-free fish grown under…
Researchers from the Institute of Photonic Sciences (ICFO), in collaboration with the CSIC and Macquarie University in Australia, have developed a new…
Developing tiny probes for research and brain-related medical applications – that is the goal of the project NeuroSeeker, launched in February 2013 with a…
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