Microarray rapid test speeds up detection in case of Legionella pneumophila outbreak

Sensor image indicating Legionella pneumophila serogroup 1, strain Bellingham-1 Image: Catharina Kober / TUM

Legionella are rod-shaped bacteria that can cause life-threatening pneumonia in humans. They multiply in warm water and can be dispersed into the air via cooling towers, evaporative recooling systems and hot water systems.

The most dangerous among the almost 50 species of Legionella is a subtype of Legionella pneumophila. It is responsible for 90 percent of all infections. When an outbreak occurs, the source of the germs must be identified as soon as possible to prevent further infections.

Similar to a paternity test, the origin of the outbreak is confirmed when the germs in the process water of a technical system exactly match those identified in the patient. However, often numerous systems must be tested in the process, and the requisite cultivation for the test takes around ten days.

Faster detection with antibodies

Meanwhile there is a rapid test for detecting the Legionella pathogen in the clinic. It identifies the compounds formed by Legionella in the urine of patients. “Unfortunately, this quick test serves only as a first indication and is not suitable for screening the water of technical systems,” says PD Dr. Michael Seidel, head of the research group at the Chair of Analytical Chemistry and Water Chemistry of the Technical University of Munich.

The team of scientists thus developed a measuring chip in the context of the “LegioTyper” project funded by the German Federal Ministry of Education and Research. This chip not only detects the dangerous pathogen Legionella pneumophila but also identifies which of the approximately 20 subtypes is present.

Fast, inexpensive and versatile

The film-based measuring chip uses the microarray analysis platform MCR of the Munich company GWK GmbH. Using 20 different antibodies, the system provides a complete analysis within 34 minutes.

“Compared to previous measurements, the new method not only provides a huge speed advantage,” says Michael Seidel, “but is also so cheap that we can use the chip in one-time applications.”

The system can be deployed for environmental hygiene as well as clinical diagnostics. In combination with a second, DNA-based method, the system can even distinguish between dead and living Legionella pathogens. This allows the success of disinfection measures to be monitored. The project participants will present their system to the public for the first time at the Analytica 2018 trade fair in Munich (Hall 3, Booth 315).

Publication:

Wunderlich, A.; Torggler, C.; Elsaesser, D.; Lück, C.; Niessner, R.; Seidel, M.,
Rapid quantification method for Legionella pneumophila in surface water.
Analytical and Bioanalytical Chemistry 2016, 408(9), 2203-2213 – DOI: 10.1007/s00216-016-9362-x.

Kober, C.; Niessner, R.; Seidel, M.
Quantification of viable and non-viable Legionella spp. by heterogeneous asymmetric recombinase polymerase amplification (haRPA) on a flow-based chemiluminescence microarray. Biosensors and Bioelectronics, 2018, 100, 49-55. https://doi.org/10.1016/j.bios.2017.08.053

More information:

The LegioTyper project is funded by the German Federal Ministry of Education and Research in the context of the “Civil Security – Protection against biological threats and pandemics” program. The antibodies were provided by the German Reference Laboratory for Legionella at the TU Dresden.

The microarray analysis platform MCR 3 of the Munich company GWK GmbH is also used in a test for antibiotic residues in milk developed at the same chair:
https://www.tum.de/die-tum/aktuelles/pressemitteilungen/detail/article/31075/

Contact:

PD Dr. Michael Seidel
Technical University of Munich
Chair of Analytical Chemistry and Water Chemistry
Institute of Hydrochemistry
Marchioninistr. 17, 81377 München, Germany
Phone: +49 89 2180 78252 – E-mail: michael.seidel@ch.tum.de
Web: http://www.hydrochemistry.tum.de/home/

https://www.tum.de/nc/en/about-tum/news/press-releases/detail/article/34535/ Link to the press release

Media Contact

Dr. Ulrich Marsch Technische Universität München

All latest news from the category: Life Sciences and Chemistry

Articles and reports from the Life Sciences and chemistry area deal with applied and basic research into modern biology, chemistry and human medicine.

Valuable information can be found on a range of life sciences fields including bacteriology, biochemistry, bionics, bioinformatics, biophysics, biotechnology, genetics, geobotany, human biology, marine biology, microbiology, molecular biology, cellular biology, zoology, bioinorganic chemistry, microchemistry and environmental chemistry.

Back to home

Comments (0)

Write a comment

Newest articles

The Sound of the Perfect Coating

Fraunhofer IWS Transfers Laser-based Sound Analysis of Surfaces into Industrial Practice with “LAwave”. Sound waves can reveal surface properties. Parameters such as surface or coating quality of components can be…

Customized silicon chips

…from Saxony for material characterization of printed electronics. How efficient are new materials? Does changing the properties lead to better conductivity? The Fraunhofer Institute for Photonic Microsystems IPMS develops and…

Acetylation: a Time-Keeper of glucocorticoid Sensitivity

Understanding the regulatory mechanism paves the way to enhance the effectiveness of anti-inflammatory therapies and to develop strategies to counteract the negative effects of stress- and age-related cortisol excess. The…

Partners & Sponsors