Forum for Science, Industry and Business

Sponsored by:     3M 
Search our Site:

 

Doing More with Less: Stem Cells Regulate Their Fate by Altering Their Stiffness

14.01.2019

In adults, mesenchymal stems cells (MSCs) are primarily found in bone marrow and they play a vital role in repair of damaged organs. The transformation of a single MSC into complex tissue like cartilage and bone starts with its association with other MSCs in order to form microscopic clusters via a process termed as condensation.

While it is known that this condensation step is important for skeletal development the exact role it plays in formation of bone and cartilage is not understood. A team led by Prof. Dr. Prasad Shastri and Dr. Melika Sarem of the Institute for Macromolecular Chemistry at the University of Freiburg present evidence for autonomous control of chondrogenesis in MSCs. These findings are published in the journal “Stem Cell Research & Therapy“.


Immunofluorescence staining of matrix proteins in engineered cartilage

Source: Institut für Makromolekulare Chemie

They discovered that reducing the number of cells participating in condensation process leads to the activation of an intrinsic differentiation program. This prompts MSCs to become cartilage cells even in absence of chondro-inductive growth factors irrespective of donor age and sex. Sarem and Shastri further identified that two cell-membrane proteins, Caveolin-1 and N-Cadherin are differentially regulated during the condensation step and function as interactive forces like a Yin-Yang of chondrogenic differentiation.

"The fact that we need lesser cells to create better quality tissue is extremely exciting as it opens new avenues for stem cell therapies" summarizes Sarem.

In collaboration with Dr. Oliver Otto, at the University of Greifswald, the Freiburg researchers demonstrated that the chondrogenic potential of MSCs correlates with emergence of a stiffer phenotype and increase in cell size. Their findings allude to a hitherto unknown mechanobiology paradigm in MSC differentiation.

“Since MSCs harvested from adult bone marrow are a heterogeneous population of cells and their ability to undergo differentiation into cartilage or bone cells varies from donor to donor, our findings have significant implication for MSC-based strategies for engineering cartilage and bone tissue,” explains Shastri.

Prasad Shastri is the Professor of Biofunctional Macromolecular Chemistry at the Institute for Macromolecular Chemistry and the Professor of Cell Signalling Environments in the Cluster of Excellence BIOSS Centre for Biological Signalling Studies at the University of Freiburg.

Original publication:
Sarem, M., Otto, O., Tanaka, S., Shastri, V. P. (2019): Cell number in mesenchymal stem cell aggregates dictates cell stiffness an chondrogenesis. In: Stem Cell Research & Therapy. DOI: 10.1186/s13287-018-1103-y

Caption:
Immunofluorescence staining of matrix proteins in engineered cartilage

Contact:
Prof. Dr. Prasad Shastri
Institut für Makromolekulare Chemie & BIOSS Centre for Biological Signalling Studies
University of Freiburg
Tel.: 0761/203-6271
E-Mail: prasad.shastri@makro.uni-freiburg.de

Originalpublikation:

https://www.pr.uni-freiburg.de/pm-en/press-releases-2018/doing-more-with-less?se...

Rudolf-Werner Dreier | idw - Informationsdienst Wissenschaft

More articles from Life Sciences:

nachricht First SARS-CoV-2 genomes in Austria openly available
03.04.2020 | CeMM Forschungszentrum für Molekulare Medizin der Österreichischen Akademie der Wissenschaften

nachricht Do urban fish exhibit impaired sleep? Light pollution suppresses melatonin production in European perch
03.04.2020 | Leibniz-Institut für Gewässerökologie und Binnenfischerei (IGB)

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Harnessing the rain for hydrovoltaics

Drops of water falling on or sliding over surfaces may leave behind traces of electrical charge, causing the drops to charge themselves. Scientists at the Max Planck Institute for Polymer Research (MPI-P) in Mainz have now begun a detailed investigation into this phenomenon that accompanies us in every-day life. They developed a method to quantify the charge generation and additionally created a theoretical model to aid understanding. According to the scientists, the observed effect could be a source of generated power and an important building block for understanding frictional electricity.

Water drops sliding over non-conducting surfaces can be found everywhere in our lives: From the dripping of a coffee machine, to a rinse in the shower, to an...

Im Focus: A sensational discovery: Traces of rainforests in West Antarctica

90 million-year-old forest soil provides unexpected evidence for exceptionally warm climate near the South Pole in the Cretaceous

An international team of researchers led by geoscientists from the Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research (AWI) have now...

Im Focus: Blocking the Iron Transport Could Stop Tuberculosis

The bacteria that cause tuberculosis need iron to survive. Researchers at the University of Zurich have now solved the first detailed structure of the transport protein responsible for the iron supply. When the iron transport into the bacteria is inhibited, the pathogen can no longer grow. This opens novel ways to develop targeted tuberculosis drugs.

One of the most devastating pathogens that lives inside human cells is Mycobacterium tuberculosis, the bacillus that causes tuberculosis. According to the...

Im Focus: Physicist from Hannover Develops New Photon Source for Tap-proof Communication

An international team with the participation of Prof. Dr. Michael Kues from the Cluster of Excellence PhoenixD at Leibniz University Hannover has developed a new method for generating quantum-entangled photons in a spectral range of light that was previously inaccessible. The discovery can make the encryption of satellite-based communications much more secure in the future.

A 15-member research team from the UK, Germany and Japan has developed a new method for generating and detecting quantum-entangled photons at a wavelength of...

Im Focus: Junior scientists at the University of Rostock invent a funnel for light

Together with their colleagues from the University of Würzburg, physicists from the group of Professor Alexander Szameit at the University of Rostock have devised a “funnel” for photons. Their discovery was recently published in the renowned journal Science and holds great promise for novel ultra-sensitive detectors as well as innovative applications in telecommunications and information processing.

The quantum-optical properties of light and its interaction with matter has fascinated the Rostock professor Alexander Szameit since College.

All Focus news of the innovation-report >>>

Anzeige

Anzeige

VideoLinks
Industry & Economy
Event News

13th AKL – International Laser Technology Congress: May 4–6, 2022 in Aachen – Laser Technology Live already this year!

02.04.2020 | Event News

“4th Hybrid Materials and Structures 2020” takes place over the internet

26.03.2020 | Event News

Most significant international Learning Analytics conference will take place – fully online

23.03.2020 | Event News

 
Latest News

Capturing 3D microstructures in real time

03.04.2020 | Materials Sciences

First SARS-CoV-2 genomes in Austria openly available

03.04.2020 | Life Sciences

Do urban fish exhibit impaired sleep? Light pollution suppresses melatonin production in European perch

03.04.2020 | Life Sciences

VideoLinks
Science & Research
Overview of more VideoLinks >>>