Such remnants have long been thought to be the source of cosmic ray particles hitting Earth. The observations show, for the first time, the presence of “seed…
When the ice melts, as was the case during the ice minimum in 2012, these algae sink rapidly to the bottom of the sea at a depth of several thousands of…
The wide use of recombinant polypeptides is limited by still insufficient means of production, namely, their expression in host cells. The most often used bacterial host cells generally have the considerable drawback that polypeptides of eukaryotic origin are not folded correctly and that undesirable posttranslational modifications may occur. One of the most severe drawbacks is that the target polypeptide is typically located in the cytoplasm of the host cells and must be isolated from said cytoplasm in a laborious, time-consuming and expensive downstream-processing.
In view of the above, an expression system is needed that is usable in an eukaryotic cell extensively autologous with respect to sugars, amino acids and that is extensively independent of growth factors. It has been shown that plant cells, in particular microalgae cells, can be used for the production of recombinant proteins and overcome most of the above problems.
Microalga cultures are comparably easy to handle and, in principle, scalable to large production scales. Furthermore, microalgae merely require a slightly salty aqueous environment, CO2 and light, can be supplemented by simple carbon sources, or a combination of these strategies to grow rapidly and produce the recombinant protein of interest. Therefore, the cultivation of microalgae is relatively inexpensive. These cultures can be extensively free of sugars and amino acids. The use of solar energy to produce biomass and recombinant protein production is an attractive aspect of these cells in light of the goal of sustainability.
Unfortunately, the efficient production of recombinant proteins in microalgae is hampered because of the tightly regulated gene expression, preference for certain nucleic acid codon usage, and bias to its own promoters.
The present invention allows to overcome these problems with highly efficient production and direct secretion of target polypeptides into the medium and in addition enables simultaneous monitoring of the polypeptide production without disturbing the expression system.
This system has in principle a large range of applications and may be used due to the safe organism for researchers, pharmaceutical targets and food and feed producers.
The wide use of recombinant polypeptides is limited by still insufficient means of production, namely, their expression in host cells. The most often used…
Diabetes mellitus is the most expensive chronic disorder, causing costs of about 40 billion Euros in 2010. More than 3/4 of these costs are associated with the…
Human filariasis (elephantiasis and river blindness) is caused by the nematodes W. bancrofti and O. volvulus, transmitted via blood sucking insects. The worms…
Dirofilariasis is caused by Dirofilaria immitis and D. repens, parasitic nematodes, which are transmitted by blood sucking mosquitoes. The heartworms harbour…
articularly in oncology determination of gene mutation statuses serve as a biomarker to guide therapeutic decisions.
Scientists of the University of…
Part of the inflammatory response in Alzheimer´s disease (AD) is the upregulation of the inducible nitric oxide synthase NOS2 resulting in increased NO…
The ECRG4 and ITIH5 (EI) gene promoter methylation has been identified as a novel objective biomarker panel for the diagnosis of bladder cancer (BLA) at an…
They can already stand, walk, wriggle under obstacles, and change colors. Now researchers are adding a new skill to the soft robot arsenal: jumping.Using small…
The basis of sexual reproduction is that a fertilized egg gets half its chromosomes from each parent — sperm and eggs each contributing one partner in each…
Scientists have long believed that the distribution of plants within a marsh is a passive adaption in which species grow at different elevations because that's…
In 2008, nearly 300,000 Chinese children were hospitalised with kidney disease brought on by supplies of powdered milk deliberately contaminated with melamine…
Ponies have a maximum height of 148 centimetres. When the height at the withers is greater, they are deemed to be large horses. Scientists at the Institute for…
What if the same technology could peer down to the level of atoms? Doctors could make visual diagnoses of a person’s molecules – examining damage on a strand…
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