Schizophrenia is among the most severe forms of mental illness, yet some people with the disease are as happy as those in good physical and mental health…
Researchers at the Beckman Institute at the University of Illinois at Urbana-Champaign have developed a new technique that can noninvasively image the pulse…
Mice missing two important proteins of the vascular system develop normally and appear healthy in adulthood, as long as they don’t become injured. If they do,…
Fish species native to a major Arizona watershed may lose access to important segments of their habitat by 2050 as surface water flow is reduced by the effects…
Half of the Earth's land mass is made up of rangelands, which include grasslands and savannas, yet they are being transformed at an alarming rate. Woody…
The internal body clocks, called circadian clocks, regulate the daily “rhythms” of many bodily functions, from waking and sleeping to body temperature and…
The work was published Aug. 12 in the International Journal of Climatology in an article titled “Creating a topoclimatic daily air temperature dataset for the…
It could be a fun party trick – put your cell phone down on a table and watch it fade into the woodwork – or part of a lifesaving technology used by industry…
A new NOAA nationwide analysis shows that between 1996 and 2011, 64,975 square miles in coastal regions–an area larger than the state of…
Thanks to a partnership between the Canadian Space Agency (CSA), MacDonald, Dettwiler and Associates Ltd. (MDA), the prime contractor for the RADARSAT-2…
Acute lymphoblastic leukemia (ALL) is the most common cancer of childhood. This disease hinders the development of healthy blood cells while cancer cells…
Scientists have long attempted to find out why these drastic temperature jumps of up to ten degrees took place in the far northern latitudes within just a few…
A research group from the Department of Biomedicine at the University and the University Children’s Hospital of Basel now discovered that innate lymphoid cells…
The trap was built by Latvian and German biologists and designed to capture bats en route when migrating along the shore of the Baltic sea to central and…
Nearly all black holes fall into one of two classes: big, and colossal. Astronomers know that black holes ranging from about 10 times to 100 times the…
Background
Using the electroporation process, electrical pulses create pores that allow genetic material to permeate the bacterial membrane of electrocompetent cells. The limiting factor for this standard laboratory method is the efficiency by which DNA can be introduced into E. coli. Commercially available electrocompetent cells are being delivered frozen at about -70 to -80°C. The conventional wisdom is that storage at a higher temperature, for example at -20°C or higher, will result in a significant decrease in viability and transformation efficiency.
Invention
Inventors of Saarland University have established a new method and created a new bacterial strain to generate electrocompetent bacterial cells with a transformation efficiency of up to 5,6 x 1010, which can be handled at room temperature. This electrocompetent cells can be stored at 4°C without losing transformation efficiency. Also the new established protocol is able to improve the transformation efficiencies of commercially available bacterial cells.
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